2024-03-29T11:56:56Zhttp://diposit.ub.edu/dspace-oai/requestoai:diposit.ub.edu:2445/1030432024-03-12T17:02:13Zcom_2445_59248com_2445_7181com_2445_21591col_2445_104466col_2445_21592
00925njm 22002777a 4500
dc
Borrell Hernández, Jordi
author
Montero Barrientos, Ma. Teresa
author
Vázquez González, Martha Leticia
author
Domènech Cabrera, Òscar
author
2016
In this work lactose permease (LacY) of Escherichia coli has been taken as model efflux pump to investigate the interactions between the protein and the main lipid components (POPE and POPG) of the inner membrane. Two main approaches have been followed: (i) measuring the fluorescence energy transfer between a single tryptophan mutant of the protein (W151/C154G LacY) and pyrene labeled phospholipids (Pyr-PE and Pyr-PG); and (ii) pulling the protein from the supported lipid bilayers where it is embedded by using the tip of the atomic force microscope (AFM). On one hand, fluorescence measurements at different pHs indicate that LacY present selectivity for PE. On the other hand the observations of the reconstituted protein in lipid bilayers by AFM show a preference of LacY for the fluid phase (Lα) rather than for the gel phase (Lβ). To get an estimation of the proportion of each lipid in each phase we have constructed a phase diagram for the system POPE:POPG. The diagram shows that at the temperature of the experiments (24 ºC) there is an almost equimolar proportion of each lipid. The results suggest the existence of a boundary region around LacY formed mainly by POPE laterally segregated from a bulk with a random distribution of POPE and POPG. Force spectroscopy allows to establish the force required and the mechanism to unspecifically unfold the protein.
http://hdl.handle.net/2445/103043
The lipid-protein interplay in efflux plump
oai:diposit.ub.edu:2445/1208772024-03-12T17:02:13Zcom_2445_21591com_2445_7181com_2445_59248com_2445_20402com_2445_7188col_2445_21592col_2445_104466col_2445_21593col_2445_32187
00925njm 22002777a 4500
dc
Folch, Jaume
author
Ettcheto Arriola, Miren
author
Busquets Figueras, Oriol
author
Sánchez-López, E. (Elena)
author
Castro-Torres, Rubén Darío
author
Beas Zárate, Carlos
author
Pallàs i Llibería, Mercè, 1964-
author
Olloquequi, Jordi
author
Jara, Daniela
author
García López, María Luisa
author
Auladell i Costa, M. Carme
author
Camins Espuny, Antoni
author
2017
http://hdl.handle.net/2445/120877
Strategies against β-amyloid protein as therapeutics in Alzheimer's disease
oai:diposit.ub.edu:2445/1743762024-03-12T17:02:13Zcom_2445_59248com_2445_7181com_2445_9244col_2445_104466col_2445_21585
00925njm 22002777a 4500
dc
Garcia Amorós, Jaume
author
Velasco Castrillo, Dolores
author
2012-09-12
Liquid crystals (LCs) are unique materials with amazing properties and uses. Since their
discovery in 1889 by F. Reinitzer1, they have experienced an explosive growth because of
their successful application in a wide variety of areas such as information displays2,
cosmetics and health care3, thermography4-5, artificial muscle-like actuators6-7 and
enantioselective synthesis8, among others. Thus, liquid crystals play an important role in
modern technology and they are present in the most common devices used in our daily live.
Research into this field is growing day by day and new promising applications for such
materials are discovered and developed continuously...
http://hdl.handle.net/2445/174376
264728
Polysiloxane Side-Chain Azobenzene-Containing Liquid Single Crystal Elastomers for Photo-Active Artificial