2024-03-28T16:35:51Zhttp://diposit.ub.edu/dspace-oai/requestoai:diposit.ub.edu:2445/1083512024-03-12T17:02:13Zcom_2445_7198com_2445_7181com_2445_96580col_2445_7199col_2445_108487
00925njm 22002777a 4500
dc
Martín-Peláez, Sandra
author
Castañer, Olga
author
Solà, Rosa
author
Motilva, María José
author
Castell, Margarida
author
Pérez-Cano, Francisco J.
author
Fitó Colomer, Montserrat
author
2016-04-01
Olive oil (OO) phenolic compounds (PC) are able to influence gut microbial populations and metabolic output. Our aim was to investigate whether these compounds and changes affect the mucosal immune system. In a randomized, controlled, double blind cross-over human trial, for three weeks, preceded by two-week washout periods, 10 hypercholesterolemic participants ingested 25 mL/day of three raw virgin OO differing in their PC concentration and origin: (1) an OO containing 80 mg PC/kg (VOO); (2) a PC-enriched OO containing 500 mg PC/kg from OO (FVOO); and (3) a PC-enriched OO containing a mixture of 500 mg PC/kg from OO and thyme (1: 1, FVOOT). Intestinal immunity (fecal immunoglobulin A (IgA) and IgA-coated bacteria) and inflammation markers (C-reactive protein (CRP) and fecal interleukin 6 (IL-6), tumor necrosis factor alpha (TNF alpha) and calprotectin) was analyzed. The ingestion of high amounts of OO PC, as contained in FVOO, tended to increase the proportions of IgA-coated bacteria and increased plasma levels of CRP. However, lower amounts of OO PC (VOO) and the combination of two PC sources (FVOOT) did not show significant effects on the variables investigated. Results indicate a potential stimulation of the immune system with very high doses of OO PC, which should be further investigated.
2072-6643
http://hdl.handle.net/2445/108351
667786
27077879
Influence of Phenol-Enriched Olive Oils on Human Intestinal Immune Function
oai:diposit.ub.edu:2445/1083622024-03-12T17:02:13Zcom_2445_7198com_2445_7181com_2445_96580col_2445_7199col_2445_108487
00925njm 22002777a 4500
dc
Rigo Adrover, Maria del Mar
author
Franch i Masferrer, Àngels
author
Castell, Margarida
author
Pérez-Cano, Francisco J.
author
2016-11-07
This study aimed to investigate the effect of supplementation with the probiotic Bifidobacterium breve M-16V on the maturation of the intestinal and circulating immune system during suckling. In order to achieve this purpose, neonatal Lewis rats were supplemented with the probiotic strain from the 6th to the 18th day of life. The animals were weighed during the study, and faecal samples were obtained and evaluated daily. On day 19, rats were euthanized and intestinal wash samples, mesenteric lymph node (MLN) cells, splenocytes and intraepithelial lymphocytes (IEL) were obtained. The probiotic supplementation in early life did not modify the growth curve and did not enhance the systemic immune maturation. However, it increased the proportion of cells bearing TLR4 in the MLN and IEL, and enhanced the percentage of the integrin αEβ7+ and CD62L+ cells in the MLN and that of the integrin αEβ7+ cells in the IEL, suggesting an enhancement of the homing process of naïve T lymphocytes to the MLN, and the retention of activated lymphocytes in the intraepithelial compartment. Interestingly, B. breve M-16V enhanced the intestinal IgA synthesis. In conclusion, supplementation with the probiotic strain B. breve M-16V during suckling improves the development of mucosal immunity in early life
1932-6203
http://hdl.handle.net/2445/108362
665371
27820846
Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life
oai:diposit.ub.edu:2445/1185092024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Garcia Aloy, Mar
author
Rabassa Bonet, Montserrat
author
Casas-Agustench, Patricia
author
Hidalgo-Liberona,Nicole
author
Llorach, Rafael
author
Andrés Lacueva, Ma. Cristina
author
2017-04-29
BACKGROUND: Accurate measurement of food intake is the cornerstone of understanding the links between diet and optimal health status or risk of disease. The utilization of metabolomics approaches is revolutionizing the field of dietary assessment by associating metabolic profiles with intake of specific foods or dietary patterns and/or investigating human health status in nutritional trials. Combining dietary biomarkers with conventional dietary assessment methods is considered a potential strategy for tackling the complexity of dietary exposure fingerprinting. SCOPE AND APPROACH: We discuss existing approaches among dietary assessment methods and dietary biomarkers. A combined approach taking into consideration data from dietary questionnaires with measurements of dietary biomarkers is emphasized. KEY FINDINGS AND CONCLUSIONS: Trends in novel strategies for improving dietary exposure assessment will be influenced by the discovery and validation of dietary exposure biomarkers. Among different strategies, multi-metabolite biomarker panels enable more reliable estimation of dietary exposure than does the traditional single-biomarker approach. Therefore, a combined approach using data from dietary questionnaires along with measurements of dietary biomarkers is considered an excellent strategy for improving dietary exposure assessment.
0924-2244
http://hdl.handle.net/2445/118509
672609
Novel strategies for improving dietary exposure assessment: Multiple-data fusion is a more accurate measure than the traditional single-biomarker approach
oai:diposit.ub.edu:2445/1213152024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7198col_2445_108487col_2445_7199
00925njm 22002777a 4500
dc
Camps i Bossacoma, Mariona
author
Franch i Masferrer, Àngels
author
Pérez-Cano, Francisco J.
author
Castell, Margarida
author
2017-06-06
Polyphenols, widely found in edible plants, influence the immune system. Nevertheless, the immunomodulatory properties of hesperidin, the predominant flavanone in oranges, have not been deeply studied. To establish the effect of hesperidin on in vivo immune response, two different conditions of immune system stimulations in Lewis rats were applied. In the first experimental design, rats were intraperitoneally immunized with ovalbumin (OVA) plus Bordetella pertussis toxin and alum as the adjuvants, and orally given 100 or 200 mg/kg hesperidin. In the second experimental design, rats were orally sensitized with OVA together with cholera toxin and fed a diet containing 0.5% hesperidin. In the first approach, hesperidin administration changed mesenteric lymph node lymphocyte (MLNL) composition, increasing the TCRαβ+ cell percentage and decreasing that of B lymphocytes. Furthermore, hesperidin enhanced the interferon (IFN)-γ production in stimulated MLNL. In the second approach, hesperidin intake modified the lymphocyte composition in the intestinal epithelium (TCRγδ+ cells) and the lamina propria (TCRγδ+, CD45RA+, natural killer, natural killer T, TCRαβ+CD4+, and TCRαβ+CD8+ cells). Nevertheless, hesperidin did not modify the level of serum anti-OVA antibodies in either study. In conclusion, hesperidin does possess immunoregulatory properties in the intestinal immune response, but this effect is not able to influence the synthesis of specific antibodies.
2072-6643
http://hdl.handle.net/2445/121315
674372
28587283
Influence of hesperidin on the systemic and intestinal rat immune response
oai:diposit.ub.edu:2445/1245292024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7198col_2445_108487col_2445_7199
00925njm 22002777a 4500
dc
Camps i Bossacoma, Mariona
author
Massot Cladera, Malen
author
Abril Gil, Maria del Mar
author
Franch i Masferrer, Àngels
author
Pérez-Cano, Francisco J.
author
Castell, Margarida
author
2017-06-27
The ability of cocoa to interact with the immune system in vitro and in vivo has been described. In the latter context, a cocoa-enriched diet in healthy rats was able to modify the immune system's functionality. This fact could be observed in the composition and functionality of lymphoid tissues, such as the thymus, spleen, and lymph nodes. Consequently, immune effector mechanisms, such as antibody synthesis, were modified. A cocoa-enriched diet in young rats was able to attenuate the serum levels of immunoglobulin (Ig) G, IgM, and IgA and also the intestinal IgM and IgA secretion. Moreover, in immunized rats, the intake of cocoa decreased specific IgG1, IgG2a, IgG2c, and IgM concentrations in serum. This immune-regulator potential was then tested in disease models in which antibodies play a pathogenic role. A cocoa-enriched diet was able to partially prevent the synthesis of autoantibodies in a model of autoimmune arthritis in rats and was also able to protect against IgE and T helper 2-related antibody synthesis in two rat models of allergy. Likewise, a cocoa-enriched diet prevented an oral sensitization process in young rats. In this review, we will focus on the influence of cocoa on the acquired branch of the immune function. Therefore, we will focus on how a cocoa diet influences lymphocyte function both in the systemic and intestinal immune system. Likewise, its potential role in preventing some antibody-induced immune diseases is also included. Although further studies must characterize the particular cocoa components responsible for such effects and nutritional studies in humans need to be carried out, cocoa has potential as a nutraceutical agent in some hypersensitivity status.
2296-861X
http://hdl.handle.net/2445/124529
674376
28702458
Cocoa diet and antibody immune response in preclinical studies
oai:diposit.ub.edu:2445/1245302024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7198col_2445_108487col_2445_7199
00925njm 22002777a 4500
dc
Camps i Bossacoma, Mariona
author
Pérez-Cano, Francisco J.
author
Franch i Masferrer, Àngels
author
Untersmayr, E.
author
Castell, Margarida
author
2017-04
Previous studies have attributed to the cocoa powder the capacity to attenuate the immune response in a rat oral sensitization model. To gain a better understanding of cocoa-induced mechanisms at small intestinal level, 3-week-old female Lewis rats were fed either a standard diet or a diet containing 10% cocoa for 4 weeks with or without concomitant oral sensitization with ovalbumin (OVA). Thereafter, we evaluated the lymphocyte composition of the Peyer's patches (PPL), small intestine epithelium (IEL) and lamina propria (LPL). Likewise, gene expression of several immune molecules was quantified in the small intestine. Moreover, histological samples were used to evaluate the proportion of goblet cells, IgA+ cells and granzyme+ cells as well. In cocoa-fed animals, we identified a five time reduction in the percentage of IgA+ cells in intestinal tissue together with a decreased proportion of TLR4+ IEL. Analyzing the lymphocyte composition, almost a double proportion of TCRalpha-beta+ cells and an increase of NK cell percentage in PPL and IEL were found. In addition, a rise in CD25+, CD103+ and CD62L- cell proportions was observed in CD4+ PPL from cocoa-fed animals, along with a decrease in gene expression of CD11b, CD11c and IL-10. These results suggest that changes in PPL and IEL composition and in the gene expression induced by the cocoa diet could be involved, among other mechanisms, on its tolerogenic effect.
0955-2863
http://hdl.handle.net/2445/124530
668173
28189917
Effect of a cocoa diet on the small intestine and gut-associated lymphoid tissue composition in an oral sensitization model in rats
oai:diposit.ub.edu:2445/1482572024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580com_2445_7198com_2445_107205col_2445_7210col_2445_108487col_2445_7199col_2445_107206
00925njm 22002777a 4500
dc
Zerón-Rugerio, María Fernanda
author
Hernáez Camba, Álvaro
author
Porras Loaiza, Armida Patricia
author
Cambras Riu, Trinitat
author
Izquierdo Pulido, Maria
author
2019-12-06
The timing of food intake has been associated with obesity and adverse metabolic outcomes, independently of the amount or content of food intake and activity level. However, the impact of the variability in the timing of food intake between weekends and weekdays on BMI (body mass index) remains unexplored. To address that, we propose to study a marker of the variability of meal timing on weekends versus weekdays (denominated as 'eating jet lag') that could be associated with increments in BMI. This cross-sectional study included 1106 subjects (aged 18-25 years). Linear regression models were used to examine the associations of eating jet lag with BMI and circadian related variables (including chronotype, eating duration, sleep duration, and social jet lag). Subsequently, a hierarchical multivariate regression analysis was conducted to determine whether the association of eating jet lag with BMI was independent of potentially confounding variables (e.g., chronotype and social jet lag). Moreover, restricted cubic splines were calculated to study the shape of the association between eating jet lag and BMI. Our results revealed a positive association between eating jet lag and BMI (p = 0.008), which was independent of the chronotype and social jet lag. Further analysis revealed the threshold of eating jet lag was of 3.5 h or more, from which the BMI could significantly increase. These results provided evidence of the suitability of the eating jet lag, as a marker of the variability in meal timing between weekends and weekdays, for the study of the influence of meal timing on obesity. In a long run, the reduction of the variability between meal timing on weekends versus weekdays could be included as part of food timing guidelines for the prevention of obesity among general population.
2072-6643
http://hdl.handle.net/2445/148257
693574
31817568
Eating jet lag: A marker of the variability in meal timing and its association with body mass index
oai:diposit.ub.edu:2445/1501542024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
García Conesa, María Teresa
author
Chambers, Karen
author
Combet, Emilie
author
Pinto, Paula
author
Garcia Aloy, Mar
author
Andrés Lacueva, Ma. Cristina
author
Pascual Teresa, Sonia de
author
Mena, Pedro
author
Konic Ristic, Aleksandra
author
Hollands, Wendy J.
author
Kroon, Paul A.
author
Rodriguez-Mateos, Ana
author
Istas, Geoffrey
author
Kontogiorgis, Christos A.
author
Rai, Dilip K.
author
Gibney, Eileen R.
author
Morand, Christine
author
Espín, Juan Carlos
author
González-Sarrías, Antonio
author
2018-02-28
Understanding interindividual variability in response to dietary polyphenols remains essential to elucidate their effects on cardiometabolic disease development. A meta-analysis of 128 randomized clinical trials was conducted to investigate the effects of berries and red grapes/wine as sources of anthocyanins and of nuts and pomegranate as sources of ellagitannins on a range of cardiometabolic risk biomarkers. The potential influence of various demographic and lifestyle factors on the variability in the response to these products were explored. Both anthocyanin- and ellagitannin-containing products reduced total-cholesterol with nuts and berries yielding more significant effects than pomegranate and grapes. Blood pressure was significantly reduced by the two main sources of anthocyanins, berries and red grapes/wine, whereas waist circumference, LDL-cholesterol, triglycerides, and glucose were most significantly lowered by the ellagitannin-products, particularly nuts. Additionally, we found an indication of a small increase in HDL-cholesterol most significant with nuts and, in flow-mediated dilation by nuts and berries. Most of these effects were detected in obese/overweight people but we found limited or non-evidence in normoweight individuals or of the influence of sex or smoking status. The effects of other factors, i.e., habitual diet, health status or country where the study was conducted, were inconsistent and require further investigation.
1661-6596
http://hdl.handle.net/2445/150154
683377
29495642
Meta-Analysis of the Effects of Foods and Derived Products Containing Ellagitannins and Anthocyanins on Cardiometabolic Biomarkers: Analysis of Factors Influencing Variability of the Individual Responses
oai:diposit.ub.edu:2445/1510102024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580com_2445_7198col_2445_7210col_2445_108487col_2445_7199
00925njm 22002777a 4500
dc
Azagra Boronat, Ignasi
author
Tres Oliver, Alba
author
Massot Cladera, Malen
author
Franch i Masferrer, Àngels
author
Castell, Margarida
author
Guardiola Ibarz, Francesc
author
Pérez-Cano, Francisco J.
author
Rodríguez Lagunas, María José
author
2020-01-25
The present study aimed to analyze the rat breast milk profile of fatty acids (FA), immunoglobulins (Ig), microbiota, and their relationship, and to further assess their associations in the mother-offspring pair. Dams were monitored during the three weeks of gestation, allowed to deliver at term, and followed during two weeks of lactation. At the end of the study, milk was obtained from the dams for the analysis of fatty acids, microbiota composition, immunoglobulins, and cytokines. Moreover, the cecal content and plasma were obtained from both the dams and pups to study the cecal microbiota composition and the plasmatic levels of fatty acids, immunoglobulins, and cytokines. Rat breast milk lipid composition was ~65% saturated FA, ~15% monounsaturated FA, and ~20% polyunsaturated FA. Moreover, the proportions of IgM, IgG, and IgA were ~2%, ~88%, and ~10%, respectively. Breast milk was dominated by members of Proteobacteria, Firmicutes, and Bacteroidetes phyla. In addition, forty genera were shared between the milk and cecal content of dams and pups. The correlations performed between variables showed, for example, that all IgGs subtypes correlated between the three compartments, evidencing their association in the mothermilk-pup line. We established the profile of FA, Ig, and the microbiota composition of rat breast milk. Several correlations in these variables evidenced their association through the mother-milkpup line. Therefore, it would be interesting to perform dietary interventions during pregnancy and/or lactation that influence the quality of breast milk and have an impact on the offspring.
2072-6643
http://hdl.handle.net/2445/151010
695258
31991792
Associations of breast milk microbiota, immune factors, and fatty acids in the rat mother-offspring pair
oai:diposit.ub.edu:2445/1510382024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_107205com_2445_7209col_2445_108487col_2445_107206col_2445_7210
00925njm 22002777a 4500
dc
Bodega, Patricia
author
Fernández Alvira, Juan Miguel
author
Santos-Beneit, Gloria
author
Cos Gandoy, Amaya de
author
Fernández Jiménez, Rodrigo
author
Moreno, Luis Alberto
author
Miguel, Mercedes de
author
Carral, Vanesa
author
Orrit, Xavier
author
Carvajal, Isabel
author
Storniolo, Carolina Emilia
author
Tresserra i Rimbau, Anna
author
Doménech, Mónica
author
Estruch Riba, Ramon
author
Lamuela Raventós, Rosa Ma.
author
Fuster, Valentín
author
2019-09-26
Previous studies on the association between dietary habits and cardiovascular risk factors (CVRF) in adolescents have generated conflicting results. The aim of this study was to describe dietary patterns (DP) in a large sample of Spanish adolescents and to assess their cross-sectional relationship with CVRF. In total, 1324 adolescents aged 12.5 ± 0.4 years (51.6% boys) from 24 secondary schools completed a self-reported food frequency questionnaire. DPs were derived by cluster analysis and principal component analysis (PCA). Anthropometric measurements, blood pressure, lipid profile, and glucose levels were assessed. Linear mixed models were applied to estimate the association between DPs and CVRF. Three DP-related clusters were obtained: Processed (29.2%); Traditional (39.1%); and Healthy (31.7%). Analogous patterns were obtained in the PCA. No overall differences in CVRF were observed between clusters except for z-BMI values, total cholesterol, and non-HDL cholesterol, with the Processed cluster showing the lowest mean values. However, differences were small. In conclusion, the overall association between DPs, as assessed by two different methods, and most analyzed CVRF was weak and not clinically relevant in a large sample of adolescents. Prospective analysis may help to disentangle the direction of these associations. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
2072-6643
http://hdl.handle.net/2445/151038
693606
31561533
Dietary patterns and Cardiovascular risk factors in spanish adolescents: A cross-sectional analysis of the SI! program for health promotion in secondary schools
oai:diposit.ub.edu:2445/1516822024-03-12T17:02:13Zcom_2445_7181com_2445_96580com_2445_7201col_2445_15202col_2445_108487col_2445_7202
00925njm 22002777a 4500
dc
Vu Cantero, Diem-Lan
author
Bosch, Albert
author
Pintó Solé, Rosa María
author
Guix Arnau, Susana
author
2017-02-18
Since they were identified in 1975, human astroviruses have been considered one of the most important agents of viral acute gastroenteritis in children. However, highly divergent astroviruses infecting humans have been recently discovered and associated with extra-intestinal infections. The report of cases of fatal meningitis and encephalitis, especially in immunocompromised individuals, has broadened their disease spectrum. Although zoonotic transmission among animal and human astroviruses has not been clearly recognized, the genetic similarity between some human and animal viruses makes it likely to occur. This review provides an update on the epidemiology of both classic and novel human astroviruses, and a comprehensive view on confirmed or potential association between astrovirus and human disease.
1999-4915
http://hdl.handle.net/2445/151682
668342
28218712
Epidemiology of classic and novel human astrovirus: gastroenteritis and beyond
oai:diposit.ub.edu:2445/1516862024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7188com_2445_7198col_2445_108487col_2445_7189col_2445_7199
00925njm 22002777a 4500
dc
Camps i Bossacoma, Mariona
author
Abril Gil, Maria del Mar
author
Saldaña-Ruíz, Sandra
author
Franch i Masferrer, Àngels
author
Pérez-Cano, Francisco J.
author
Castell, Margarida
author
2016-04-23
Cocoa powder, a rich source of polyphenols, has shown immunomodulatory properties in both the intestinal and systemic immune compartments of rats. The aim of the current study was to establish the effect of a cocoa diet in a rat oral sensitization model and also to gain insight into the mesenteric lymph nodes (MLN) activities induced by this diet. To achieve this, three-week-old Lewis rats were fed either a standard diet or a diet with 10% cocoa and were orally sensitized with ovalbumin (OVA) and with cholera toxin as a mucosal adjuvant. Specific antibodies were quantified, and lymphocyte composition, gene expression, and cytokine release were established in MLN. The development of anti-OVA antibodies was almost totally prevented in cocoa-fed rats. In addition, this diet increased the proportion of TCR gamma delta+ and CD103+CD8+ cells and decreased the proportion of CD62L+CD4+ and CD62L+CD8+ cells in MLN, whereas it upregulated the gene expression of OX40L, CD11c, and IL-1 beta and downregulated the gene expression of IL-17 alpha. In conclusion, the cocoa diet induced tolerance in an oral sensitization model accompanied by changes in MLN that could contribute to this effect, suggesting its potential implication in the prevention of food allergies.
2072-6643
http://hdl.handle.net/2445/151686
667785
27120615
Cocoa Diet Prevents Antibody Synthesis and Modifies Lymph Node Composition and Functionality in a Rat Oral Sensitization Model
oai:diposit.ub.edu:2445/1525822024-03-12T17:02:13Zcom_2445_7198com_2445_7181com_2445_96580col_2445_7199col_2445_108487
00925njm 22002777a 4500
dc
Lozano Mena, Glòria
author
Sánchez González, Marta
author
Juan i Olivé, M. Emília
author
Planas i Rosselló, Joana M.
author
2014-08-04
Maslinic acid is a pentacyclic triterpene found in a variety of natural sources, ranging from herbal remedies used in traditional Asian medicine to edible vegetables and fruits present in the Mediterranean diet. In recent years, several studies have proved that maslinic acid exerts a wide range of biological activities, i.e. antitumor, antidiabetic, antioxidant, cardioprotective, neuroprotective, antiparasitic and growth-stimulating. Experimental models used for the assessment of maslinic acid effects include established cell lines, which have been often used to elucidate the underlying mechanisms of action, and also animal models of different disorders, which have confirmed the effects of the triterpene in vivo. Overall, and supported by the lack of adverse effects in mice, the results provide evidence of the potential of maslinic acid as a nutraceutical, not only for health promotion, but also as a therapeutic adjuvant in the treatment of several disorders.
1420-3049
http://hdl.handle.net/2445/152582
643608
25093990
Maslinic acid, a natural phytoalexin-type triterpene from olives - a promising nutraceutical?
oai:diposit.ub.edu:2445/1576182024-03-12T17:02:13Zcom_2445_7203com_2445_7181com_2445_96580com_2445_7201col_2445_7204col_2445_108487col_2445_7202
00925njm 22002777a 4500
dc
Parrón, Ignacio
author
Álvarez, J.
author
Jané, Mireia
author
Cornejo Sánchez, Thais
author
Razquin, E.
author
Guix Arnau, Susana
author
Camps, G.
author
Pérez, C.
author
Domínguez García, Àngela
author
Working Group for the Study of Outbreaks of Acute Gastroenteritis in Catalonia
author
2019
On 16 March 2018, a nursing home notified a possible acute gastroenteritis outbreak that affected 11 people. Descriptive and case-control studies and analysis of clinical and environmental samples were carried out to determine the characteristics of the outbreak, its aetiology, the transmission mechanism and the causal food. The extent of the outbreak in and outside the nursing home was determined and the staff factors influencing propagation were studied by multivariate analysis. A turkey dinner on March 14 was associated with the outbreak (OR 4.22, 95% CI 1.11-16.01). Norovirus genogroups I and II were identified in stool samples. The attack rates in residents, staff and household contacts of staff were 23.49%, 46.22% and 22.87%, respectively. Care assistants and cleaning staff were the staff most frequently affected. Cohabitation with an affected care assistant was the most important factor in the occurrence of cases in the home (adjusted OR 6.37, 95% CI 1.13-36.02). Our results show that staff in close contact with residents and their household contacts had a higher risk of infection during the norovirus outbreak.
0950-2688
http://hdl.handle.net/2445/157618
690810
31364566
A foodborne norovirus outbreak in a nursing home and spread to staff and their household contacts
oai:diposit.ub.edu:2445/1579802024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
González-Domínguez, Raúl
author
Urpí Sardà, Mireia
author
Jáuregui Pallarés, Olga
author
Needs, Paul W.
author
Kroon, Paul A.
author
Andrés Lacueva, Ma. Cristina
author
2019-03-01
Accurate dietary assessment is a challenge in nutritional research, needing powerful and robust tools for reliable measurement of food intake biomarkers. In this work, we have developed a novel quantitative dietary fingerprinting (QDF) approach, which enables for the first time the simultaneous quantitation of about 350 urinary food-derived metabolites, including (poly)phenolic aglycones, phase II metabolites, and microbial-transformed compounds, as well as other compounds (e.g., glucosinolates, amino acid derivatives, methylxanthines, alkaloids, and markers of alcohol and tobacco consumption). This method was fully validated for 220 metabolites, yielding good linearity, high sensitivity and precision, accurate recovery rates, and negligible matrix effects. Furthermore, 127 additional phase II metabolites were also included in this method after identification in urines collected from acute dietary interventions with various foods. Thus, this metabolomic approach represents one-step further toward precision nutrition and the objective of improving the accurateness and comprehensiveness in the assessment of dietary patterns and lifestyles.
0021-8561
http://hdl.handle.net/2445/157980
690088
30799616
Quantitative Dietary Fingerprinting (QDF)-A Novel Tool for Comprehensive Dietary Assessment Based on Urinary Nutrimetabolomics
oai:diposit.ub.edu:2445/1587372024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209com_2445_20402com_2445_13750col_2445_108487col_2445_7210col_2445_20403col_2445_13751
00925njm 22002777a 4500
dc
Are, Celeste
author
Pérez Bosch, Maria
author
Ballette, Roberto
author
Proto, Stefano
author
Caso, Federica
author
Yayik, Nihan
author
Bosch Cartes, Joan
author
Amat Tusón, Mercedes
author
2019
The synthesis of enantiopure ABCE and ABCD tetracyclic advanced intermediates en route to madangamine alkaloids and studies for the construction of the triunsaturated 15-membered D ring of madangamine B and the saturated 13-membered D ring of madangamine E are reported.
0947-6539
http://hdl.handle.net/2445/158737
693283
Access to Enantiopure Advanced Intermediates en Route to Madangamines
oai:diposit.ub.edu:2445/1592032024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Quintanilla-Casas, Beatriz
author
Bustamante Alonso, Julen
author
Guardiola Ibarz, Francesc
author
García González, Diego Luís
author
Barbieri, Sara
author
Bendini, Alessandra
author
Gallina Toschi, Tullia
author
Vichi, S. (Stefania)
author
Tres Oliver, Alba
author
2019-12-07
Sensory quality, assessed following a standardized method, is one of the parameters defining the commercial category of virgin olive oil. Considering the difficulties linked to the organoleptic evaluation, especially the high number of samples to be assessed, setting up instrumental methods to support sensory panels becomes a need for the olive oil sector. Volatile fingerprint by Headspace Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry can be an excellent fit-for-purpose tool as the volatile fraction is responsible for virgin olive oil sensory attributes. A fingerprinting approach was applied to the volatile profile of 176 virgin olive oils pre- viously graded by six official sensory panels. The classification strategy consisted in two sequential Partial Least Square-Discriminant Analysis models built with the aligned chromatograms: the first discriminated extra virgin and non-extra virgin samples; the second classified the latter into virgin or lampante categories. Results were satisfactory in the cross-validation by leave 10%-out (97% of correct classification). For external validation, an uncertainty range was set for the prediction models to detect boundary samples, which would be further assessed by the sensory panels. By doing this, a considerable decrease of the panel workload (around 80%) was achieved, while maintaining a highly reliable classification of samples (error rate < 10%).
0023-6438
http://hdl.handle.net/2445/159203
695054
32204346
Virgin olive oil volatile fingerprint and chemometrics: Towards an instrumental screening tool to grade the sensory quality
oai:diposit.ub.edu:2445/1592202024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580com_2445_13750col_2445_7210col_2445_108487col_2445_13751
00925njm 22002777a 4500
dc
Sandoval, Viviana
author
Femenias, Antoni
author
Martínez Garza, Úrsula
author
Sanz Lamora, Héctor
author
Castagnini, Juan Manuel
author
Quifer Rada, Paola
author
Lamuela Raventós, Rosa Ma.
author
Marrero González, Pedro F.
author
Haro Bautista, Diego
author
Relat Pardo, Joana
author
2019-09
Maqui (Aristotelia Chilensis) berry features a unique profile of anthocyanidins that includes high amounts of delphinidin-3-O-sambubioside-5-O-glucoside and delphinidin-3-O-sambubioside and has shown positive effects on fasting glucose and insulin levels in humans and murine models of type 2 diabetes and obesity. The molecular mechanisms underlying the impact of maqui on the onset and development of the obese phenotype and insulin resistance was investigated in high fat diet-induced obese mice supplemented with a lyophilized maqui berry. Maqui-dietary supplemented animals showed better insulin response and decreased weight gain but also a differential expression of genes involved in de novo lipogenesis, fatty acid oxidation, multilocular lipid droplet formation and thermogenesis in subcutaneous white adipose tissue (scWAT). These changes correlated with an increased expression of the carbohydrate response element binding protein b (Chrebpb), the sterol regulatory binding protein 1c (Srebp1c) and Cellular repressor of adenovirus early region 1A-stimulated genes 1 (Creg1) and an improvement in the fibroblast growth factor 21 (FGF21) signaling. Our evidence suggests that maqui dietary supplementation activates the induction of fuel storage and thermogenesis characteristic of a brown-like phenotype in scWAT and counteracts the unhealthy metabolic impact of an HFD. This induction constitutes a putative strategy to prevent/treat diet-induced obesity and its associated comorbidities.
2076-3921
http://hdl.handle.net/2445/159220
693605
31480627
Lyophilized Maqui (Aristotelia chilensis) Berry Induces Browning in the Subcutaneous White Adipose Tissue and Ameliorates the Insulin Resistance in High Fat Diet-Induced Obese Mice
oai:diposit.ub.edu:2445/1594192024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Hurtado Barroso, Sara
author
Quifer Rada, Paola
author
Marhuenda-Muñoz, María
author
Rinaldi de Alvarenga, José Fernando
author
Tresserra i Rimbau, Anna
author
Lamuela Raventós, Rosa Ma.
author
2019-09
Consumption of organic products is increasing yearly due to perceived health-promoting qualities. Several studies have shown higher amounts of phytochemicals such as polyphenols and carotenoids in foods produced by this type of agriculture than in conventional foods, but whether this increase has an impact on humans still needs to be assessed. A randomized, controlled and crossover study was carried out in nineteen healthy subjects aged 18-40 years, who all followed an organic and conventional healthy diet, both for a 4-week period. Analysis of biological samples revealed a significant increase on the excretion of 4-hydroxybenzoic acid (4-HBA), a phenolic metabolite with biological activity, after the organic intervention. However, no changes were observed in the other variables analyzed. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
2076-3921
http://hdl.handle.net/2445/159419
693604
31450569
Increase of 4-Hydroxybenzoic, a Bioactive Phenolic Compound, after an Organic Intervention Diet
oai:diposit.ub.edu:2445/1597312024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Bernardi, Stefano
author
Bo', Cristian Del
author
Guglielmetti, Simone
author
Cherubini, Antonio
author
Kroon, Paul A.
author
Kirkup, Benjamin
author
Hidalgo Liberona, Nicole
author
Peron, Gregorio
author
González-Domínguez, Raúl
author
Andrés Lacueva, Ma. Cristina
author
Riso, Patrizia
author
2019-08-16
The inevitable rise of the proportion of people aged >65 years worldwide is paralleled by an increased burden of chronic diseases often associated with low-grade systemic inflammation. Recent findings suggest a link between inflammation and intestinal permeability (IP), a condition characterized by an impairment of intestinal barrier function which enables the translocation of dietary and bacterial factors into the blood activating the host immune system [1,2]. Dietary components can be significant modulators of inflammation and IP, and can also affect the intestinal microbial ecosystem. In the context of a diet-microbiota-IP axis in older subjects, dietary bioactives such as polyphenols may play a significant protective role due to their widely reported antioxidant and immunomodulatory properties and potential to regulate IP [3-6].
2504-3900
http://hdl.handle.net/2445/159731
699069
Role of a Polyphenol-Rich Dietary Pattern in the Modulation of Intestinal Permeability in Older Subjects: The MaPLE Study
oai:diposit.ub.edu:2445/1597342024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Ulaszewska, Marynka M.
author
Weinert, Christoph H.
author
Trimigno, Alessia
author
Portmann, Reto
author
Andrés Lacueva, Ma. Cristina
author
Badertscher, René
author
Brennan, Lorraine
author
Brunius, Carl
author
Bub, Achim
author
Capozzi, Francesco
author
Cialiè Rosso, Marta
author
Cordero, Chiara E.
author
Daniel, Hannelore
author
Durand, Stephanie
author
Egert, Bjoern
author
Ferrario, Paola G.
author
Feskens, Edith J. M.
author
Franceschi, Pietro
author
Garcia Aloy, Mar
author
Giacomoni, Franck
author
Giesbertz, Pieter
author
González-Domínguez, Raúl
author
Hanhineva, Kati
author
Hemeryck, Lieselot Y.
author
Kopka, Joachim
author
Kulling, Sabine
author
Llorach, Rafael
author
Manach, Claudine
author
Mattivi, Fulvio
author
Migné, Carole
author
Münger, Linda H.
author
Ott, Beate
author
Picone, Gianfranco
author
Pimentel, Grégory
author
Pujos Guillot, Estelle
author
Riccadonna, Samantha
author
Rist, Manuela
author
Rombout, Caroline
author
Rubert, Josep
author
Skurk, Thomas
author
Sri Harsha, Pedapati S. C.
author
Van Meulebroek, Lieven
author
Vanhaecke, Lynn
author
Vázquez Fresno, Rosa
author
Wishard, David
author
Vergères, Guy
author
2019
The life sciences are currently being transformed by an unprecedented wave of developments in molecular analysis, which include important advances in instrumental analysis as well as biocomputing. In light of the central role played by metabolism in nutrition, metabolomics is rapidly being established as a key analytical tool in human nutritional studies. Consequently, an increasing number of nutritionists integrate metabolomics into their study designs. Within this dynamic landscape, the potential of nutritional metabolomics (nutrimetabolomics) to be translated into a science, which can impact on health policies, still needs to be realized. A key element to reach this goal is the ability of the research community to join, to collectively make the best use of the potential offered by nutritional metabolomics. This article, therefore, provides a methodological description of nutritional metabolomics that reflects on the state‐of‐the‐art techniques used in the laboratories of the Food Biomarker Alliance (funded by the European Joint Programming Initiative "A Healthy Diet for a Healthy Life" (JPI HDHL)) as well as points of reflections to harmonize this field. It is not intended to be exhaustive but rather to present a pragmatic guidance on metabolomic methodologies, providing readers with useful "tips and tricks" along the analytical workflow.
1613-4125
http://hdl.handle.net/2445/159734
699068
30176196
Nutrimetabolomics: An Integrative Action for Metabolomic Analyses in Human Nutritional Studies
oai:diposit.ub.edu:2445/1601772024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580com_2445_7198col_2445_7210col_2445_108487col_2445_7199
00925njm 22002777a 4500
dc
Azagra Boronat, Ignasi
author
Tres Oliver, Alba
author
Massot Cladera, Malen
author
Franch i Masferrer, Àngels
author
Castell, Margarida
author
Guardiola Ibarz, Francesc
author
Pérez-Cano, Francisco J.
author
Rodríguez Lagunas, María José
author
2020-02-28
Probiotics have shown potential for their use in early life. This study aimed to investigate whether the administration of Lactobacillus fermentum CECT5716 during pregnancy and lactation periods impacts maternal and offspring plasma lipid profile, immune system and microbiota. Rats were supplemented with the probiotic during gestation and two weeks of lactation. After supplementation, although the microbiota composition was not affected, the probiotic strain was detected in all cecal contents of dams and in some of their pups. Dams showed reduced proportion of T cytotoxic cells in the mesenteric lymph nodes, modulation of intestinal cytokines (IL-10 and IL-12) and changes in plasma fatty acids (20:0, 22:0, 20:5 n-3, and 18:3 n-6). Pups showed changes in immunoglobulins (intestinal IgA and plasmatic IgG2a and IgG2c) and fatty acid profile (17:0, 22:0, and 18:2 n-6). Overall, Lactobacillus fermentum CECT5716 supplementation contributed to beneficially modulating the immune system of the mother and its offspring.
2073-4409
http://hdl.handle.net/2445/160177
699558
32121244
Lactobacillus fermentum CECT5716 Supplementation in Rats During Pregnancy and Lactation Impacts Maternal and Offspring Lipid Profile, Immune System and Microbiota
oai:diposit.ub.edu:2445/1619772024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7198com_2445_7209col_2445_108487col_2445_7199col_2445_7210
00925njm 22002777a 4500
dc
Zerón-Rugerio, María Fernanda
author
Longo-Silva, Giovana
author
Hernáez Camba, Álvaro
author
Ortega Regules, Ana Eugenia
author
Cambras Riu, Trinitat
author
Izquierdo Pulido, Maria
author
2020-02-05
Meal timing relative to sleep/wake schedules is relevant in the search for obesity risk factors. However, clock time does not accurately characterize the timing of food intake in the context of internal circadian timing. Therefore, we studied elapsed between dinner and the midpoint of sleep (TDM) as a practical approach to evaluate meal timing relative to internal timing, and its implications on obesity. To do so, adiposity, sleep, diet, physical activity, and TDM were measured in 133 women. The participants were grouped into four categories according to their sleep timing behavior (early-bed/early-rise; early-bed/late-rise; late-bed/early-rise; late-bed/late-rise). Differences among the categories were tested using ANOVA, while restricted cubic splines were calculated to study the association between TDM and adiposity. Our results show that, although participants had dinner at about the same time, those that had the shortest TDM (early-bed/early-rise group) were found to have significantly higher BMI and waist circumference values (2.3 kg/m2 and 5.2 cm) than the other groups. In addition, a TDM of 6 h was associated with the lowest values of adiposity. The TDM could be a practical approach to personalizing meal timing based on individual sleep/wake schedules. Thus, according to our findings, dining 6 h before the midpoint of sleep is an important finding and could be vital for future nutritional recommendations and for obesity prevention and treatment.
2072-6643
http://hdl.handle.net/2445/161977
696218
32033292
The elapsed time between dinner and the midpoint of sleep is associated with adiposity in young women.
oai:diposit.ub.edu:2445/1619902024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7198col_2445_108487col_2445_7199
00925njm 22002777a 4500
dc
Azagra Boronat, Ignasi
author
Massot Cladera, Malen
author
Knipping, K.
author
van't Land, B.
author
Tims, S.
author
Stahl, B.
author
Knol, J.
author
Garssen, J.
author
Franch i Masferrer, Àngels
author
Castell, Margarida
author
Pérez-Cano, Francisco J.
author
Rodríguez Lagunas, María José
author
2019-08-11
Colonization of the gut in early life can be altered through multiple environmental factors. The present study aimed to investigate the effects of 2'-fucosyllactose (2'-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2'-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system. The dietary interventions were administered daily by oral gavage at days 2-8 of life in neonatal Lewis rats. On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied. All dietary interventions showed reduction in clinical variables of RV-induced diarrhea. RV infection increased TLR2 expression, whereas 2'-FL boosted TLR5 and TLR7 expressions and scGOS/lcFOS increased that of TLR9. RV-infected rats displayed an intestinal dysbiosis that was effectively prevented by the dietary interventions, and consequently, their microbiota was more similar to microbiota of the noninfected groups. The preventive effect of 2'-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
2073-4409
http://hdl.handle.net/2445/161990
691285
31405262
Oligosaccharides Modulate Rotavirus-Associated Dysbiosis and TLR Gene Expression in Neonatal Rats
oai:diposit.ub.edu:2445/1621972024-03-12T17:02:13Zcom_2445_7198com_2445_7181com_2445_96580col_2445_7199col_2445_108487
00925njm 22002777a 4500
dc
Azagra Boronat, Ignasi
author
Massot Cladera, Malen
author
Knipping, K.
author
Garssen, J.
author
Ben Amor, Kaouther
author
Knol, J.
author
Franch i Masferrer, Àngels
author
Castell, Margarida
author
Rodríguez Lagunas, María José
author
Pérez-Cano, Francisco J.
author
2020-02-15
Probiotic supplementationwithdifferent lactobacilli andbifidobacterial strainshasdemonstrated beneficial effects in infectious diarrhea caused by rotavirus (RV) in young children. Preclinical models of RV infection might be a good strategy to screen for the e cacy of new probiotic strains or to test their comparative e cacy. Neonatal Lewis rats were supplemented with Bifidobacterium breve M-16V, Lactobacillus acidophilus NCFM, Lactobacillus helveticus R0052, or Lactobacillus salivarius PS2 from days 2-14 of life. On day five, animals received RV SA-11 orally. Fecal samples were collected daily, weighed, and scored for the calculation of severity and incidence of diarrhea. In addition, fecal pH and fecal viral shedding were measured. Animals were sacrificed at the end of the study and their blood was obtained for the quantification of RV-specific immunoglobulins. RV infection was induced in ~90% of the animals. All probiotics caused a reduction of several clinical variables of severity and incidence of diarrhea, except L. salivarius PS2. L. acidophilus NCFM, B. breve M-16V, and L. helveticus R0052 seemed to be very e ective probiotic strains. In addition, all Lactobacillus strains reduced the viral elimination one day post-inoculation. No di erences were detected in the specific anti-RV humoral response. The present study highlights the strain-specific e ects of probiotics and identifies promising probiotics for use in ameliorating and preventing RV-induced diarrhea in children, for example by including them in infant formulas
2072-6643
http://hdl.handle.net/2445/162197
699233
32075234
Strain-Specific Probiotic Properties of Bifidobacteria and Lactobacilli for the Prevention of Diarrhea Caused by Rotavirus in a Preclinical Model
oai:diposit.ub.edu:2445/1623772024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Palau Rodríguez, Magalí
author
Tulipani, Sara
author
Marco Ramell, Anna
author
Miñarro Alonso, Antonio
author
González-Domínguez, Raúl
author
Sànchez, Àlex (Sànchez Pla)
author
Ramos Molina, Bruno
author
Tinahones, Francisco J.
author
Andrés Lacueva, Ma. Cristina
author
2018-06-12
The exact impact of bariatric surgery in metabolically 'healthy' (MH) or 'unhealthy' (MU) phenotypes for the study of the metabolic improvement is still unknown. We applied an untargeted LC-ESI-TripleTOF-MS-driven metabolomics approach in serum samples from 39 patients with morbid obesity (MH and MU) 1, 3, and 6 months after bariatric surgery. Multiple factor analysis, along with correlation and enrichment analyses, was carried out to distinguish those metabolites associated with metabolic improvement. Hydroxypropionic acids, medium-/long-chain hydroxy fatty acids, and bile acid glucuronides were the most discriminative biomarkers of response between MH and MU phenotypes. Hydroxypropionic (hydroxyphenyllactic-related) acids, amino acids, and glycerolipids were the most significant clusters of metabolites altered after bariatric surgery in MU ( p < 0.001). After surgery, MU and MH changed toward a common metabolic state 3 months after surgery. We observed a negative correlation with changes in waist circumference and cholesterol levels with metabolites of lipid metabolism. Glycemic variables were correlated with hexoses, which, in turn, correlated with gluconic acid and amino acid metabolism. Finally, we noted that hydroxyphenyllactic acid was associated with amino acid and lipid metabolism. Microbial metabolism of amino acid and BA glucuronidation pathways may be the key points of metabolic rearrangement after surgery.
1535-3893
http://hdl.handle.net/2445/162377
683368
Characterization of metabolomic profile associated with metabolic improvement after bariatric surgery in subjects with morbid obesity
oai:diposit.ub.edu:2445/1623972024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7198com_2445_7209col_2445_108487col_2445_7199col_2445_7210
00925njm 22002777a 4500
dc
Camps i Bossacoma, Mariona
author
Garcia Aloy, Mar
author
Saldaña-Ruíz, Sandra
author
Cambras Riu, Trinitat
author
González-Domínguez, Raúl
author
Franch i Masferrer, Àngels
author
Pérez-Cano, Francisco J.
author
Andrés Lacueva, Ma. Cristina
author
Castell, Margarida
author
2019-03-11
Cocoa is rich in polyphenols and methylxanthines and it has been reported that its consumption, among other properties, has beneficial effects on metabolism. This study aimed to investigate the role of theobromine in cocoa's metabolic properties in healthy rats. In addition to morphometric measurements, biochemical markers of lipids and glucose metabolism and gene expression of molecules related to immune cells in adipose and hepatic tissues were assessed after 7 or 18 days of diet. Additionally, a metabolomic analysis was carried out at day 7. This study revealed the presence of six discriminant metabolites in plasma due to the diets. Moreover, the results showed that theobromine is the main responsible factor for cocoa's effects on body weight gain as well as on lipid and glucose metabolism. The effects on body weight and lipids appeared as early as after 7 days of diet, whereas those affecting glucose metabolism required a longer intervention.
0021-8561
http://hdl.handle.net/2445/162397
689222
Role of theobromine in cocoa's metabolic properties in healthy rats.
oai:diposit.ub.edu:2445/1623982024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Peron, Gregorio
author
Hidalgo-Liberona,Nicole
author
González-Domínguez, Raúl
author
Garcia Aloy, Mar
author
Guglielmetti, Simone
author
Bernardi, Stefano
author
Kirkup, Benjamin
author
Kroon, Paul A.
author
Cherubini, Antonio
author
Riso, Patrizia
author
Andrés Lacueva, Ma. Cristina
author
2019-05-14
The gut microbiota is involved in the regulation of the intestinal permeability (IP), whose disruption is a frequent condition in older people and is associated with the development of several diseases. The diet can affect the gut microbiota and IP, although the molecular mechanisms involved are unclear. Metabolomics is one of the suitable approaches to study the effects of diet on gut microbiota and IP, although, up to now, the research has focused only on a few dietary components. The aim here was to review the most recent literature concerning the application of metabolomics to the study of the diet-induced alterations of gut microbiota and the effects on IP, with a particular focus on the molecular pathways involved. An additional aim was to give a perspective on the future research involving dietary polyphenols, because despite their potential use in the management of increased IP, few studies have been reported to date.
0021-8561
http://hdl.handle.net/2445/162398
691807
Exploring the Molecular Pathways Behind the Effects of Nutrients and Dietary Polyphenols on Gut Microbiota and Intestinal Permeability: A Perspective on the Potential of Metabolomics and Future Clinical Applications.
oai:diposit.ub.edu:2445/1625582024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Hurtado Barroso, Sara
author
Martínez Huélamo, Miriam
author
Rinaldi de Alvarenga, José Fernando
author
Quifer Rada, Paola
author
Vallverdú i Queralt, Anna
author
Pérez-Fernández, Silvia
author
Lamuela Raventós, Rosa Ma.
author
2019-04-15
Sofrito is a Mediterranean tomato-based sauce that typically also contains olive oil, onion, and garlic. The preparation of sofrito modifies the bioactive compounds (carotenoids and polyphenols) in the ingredients to more bioavailable forms, promoting cis-lycopene formation and polyphenol bioaccessibility. To evaluate the health benefits of this cooking technique, the effect of consuming an acute dose of sofrito on the inflammatory status was studied. In a clinical trial, 22 healthy male subjects consumed a single dose of sofrito (240 g/70 kg) after three days without ingesting any tomato products and following a low-antioxidant diet the day before the intervention. Plasma carotenoids and total polyphenol excretion (TPE) were evaluated, as well as the inflammatory biomarkers C-reactive protein (CRP), interleukin-6 (IL-6), interleukin 1β (IL-1β) and tumor necrosis factor-α (TNF-α). After the sofrito intake, a significant decrease in CRP (p = 0.010) and TNF-α (p = 0.011) was observed, but only TNF-α was inversely correlated with an increase in TPE and plasma β-carotene (not the major carotenoid, lycopene). The positive health effects of this tomato-based product may be attributed not only to lycopene, but to the bioactive compounds of all the ingredients.
2072-6643
http://hdl.handle.net/2445/162558
689769
30991720
Acute Effect of a Single Dose of Tomato Sofrito on Plasmatic Inflammatory Biomarkers in Healthy Men
oai:diposit.ub.edu:2445/1625772024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Martínez Garza, Úrsula
author
Torres-Oteros, Daniel
author
Yarritu-Gallego, Alex
author
Marrero González, Pedro F.
author
Haro Bautista, Diego
author
Relat Pardo, Joana
author
2019-09-21
The Fibroblast Growth Factor 21 (FGF21) is considered an attractive therapeutic target for obesity and obesity-related disorders due to its beneficial effects in lipid and carbohydrate metabolism. FGF21 response is essential under stressful conditions and its metabolic effects depend on the inducer factor or stress condition. FGF21 seems to be the key signal which communicates and coordinates the metabolic response to reverse different nutritional stresses and restores the metabolic homeostasis. This review is focused on describing individually the FGF21-dependent metabolic response activated by some of the most common nutritional challenges, the signal pathways triggering this response, and the impact of this response on global homeostasis. We consider that this is essential knowledge to identify the potential role of FGF21 in the onset and progression of some of the most prevalent metabolic pathologies and to understand the potential of FGF21 as a target for these diseases. After this review, we conclude that more research is needed to understand the mechanisms underlying the role of FGF21 in macronutrient preference and food intake behavior, but also in β-klotho regulation and the activity of the fibroblast activation protein (FAP) to uncover its therapeutic potential as a way to increase the FGF21 signaling.
1661-6596
http://hdl.handle.net/2445/162577
697147
31546675
Fibroblast Growth Factor 21 and the Adaptive Response to Nutritional Challenges.
oai:diposit.ub.edu:2445/1627592024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Olmo-Cunillera, Alexandra
author
López-Yerena, Anallely
author
Lozano-Castellón, Julián
author
Tresserra i Rimbau, Anna
author
Vallverdú i Queralt, Anna
author
Pérez Bosch, Maria
author
2020-03-16
Extra virgin olive oil (EVOO), a key component of the Mediterranean Diet, has aroused interest in recent years due to its health properties. Nuclear magnetic resonance (NMR) spectroscopy is an appropriate tool for the accurate quantification of minor compounds in complex food matrices, such as polyphenols in olive oil. Flavonoids, lignans, secoiridoids and phenolic acids and alcohols in EVOO have been identified and quantified by NMR. This review provides an overview of the major developments in the structural elucidation of polyphenol compounds in EVOO.
0022-5142
http://hdl.handle.net/2445/162759
693742
NMR Spectroscopy: A powerful tool for the analysis of polyphenols in extra virgin olive oil
oai:diposit.ub.edu:2445/1629912024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Sánchez Pérez, Sònia
author
Comas Basté, Oriol
author
Rabell-González, Judit
author
Veciana Nogués, María Teresa
author
Latorre Moratalla, Mariluz
author
Vidal Carou, Ma. Carmen
author
2018-12-14
Abstract: Low-histamine diets are currently used to reduce symptoms of histamine intolerance, a disorder in histamine homeostasis that increases plasma levels, mainly due to reduced diamine-oxidase (DAO) activity. These diets exclude foods, many of them of plant origin, which patients associate with the onset of the symptomatology. This study aimed to review the existing data on histamine and other biogenic amine contents in nonfermented plant-origin foods, as well as on their origin and evolution during the storage or culinary process. The only plant-origin products with significant levels of histamine were eggplant, spinach, tomato, and avocado, each showing a great variability in content. Putrescine has been found in practically all plant-origin foods, probably due to its physiological origin. The high contents of putrescine in certain products could also be related to the triggering of the symptomatology by enzymatic competition with histamine. Additionally, high spermidine contents found in some foods should also be taken into account in these diets, because it can also be metabolized by DAO, albeit with a lower affinity. It is recommended to consume plant-origin foods that are boiled or are of maximum freshness to reduce biogenic amine intake.
2304-8158
http://hdl.handle.net/2445/162991
687060
30558197
Biogenic Amines in Plant-Origin Foods: Are they Frequently Underestimated in Low-Histamine Diets?
oai:diposit.ub.edu:2445/1630262024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Comas Basté, Oriol
author
Latorre Moratalla, Mariluz
author
Bernacchia, Roberta
author
Veciana Nogués, María Teresa
author
Vidal Carou, Ma. Carmen
author
2017-10-25
Histamine intolerance is a disorder in the homeostasis of histamine due to a reduced intestinal degradation of this amine, mainly caused by diamine oxidase (DAO) enzyme deficiency, which provokes its accumulation in plasma and the appearance of adverse health affects. A new approach for the diagnosis of this intolerance could be the determination of histamine and its metabolites in urine. The aim of this work was to develop and validate a rapid method to determine histamine and methylhistamine in human urine by Ultra High Performance Liquid Chromatography and Fluorimetric detection (UHPLC-FL). The proposed method is a consistent procedure to determine histamine and methylhistamine in less than 11 minutes with adequate linearity and sensitivity. Relative standard deviation was always lower than 5.5%, ensuring method precision; and mean recovery was greater than 99% for both analytes. The structure of histamine and methylhistamine conjugated with OPA were confirmed by UHPLC-ITD-FTMS which enabled to unequivocally identify both analytes in standards and also in urine samples. The analysis of histamine and methylhistamine in urine samples could be a potential new approach for the routine diagnosis of histamine intolerance, more patient-friendly and with clear advantages in terms of equipment and personnel demand for sample collection in comparison with current plasmatic DAO activity determination.
0731-7085
http://hdl.handle.net/2445/163026
674563
New approach for the diagnosis of histamine intolerance based on the determination of histamine and methylhistamine in urine.
oai:diposit.ub.edu:2445/1630382024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Comas Basté, Oriol
author
Latorre Moratalla, Mariluz
author
Sánchez Pérez, Sònia
author
Veciana Nogués, María Teresa
author
Vidal Carou, Ma. Carmen
author
2019-11
Intestinal diamine oxidase (DAO) acts as a protective barrier against exogenous histamine. A deficit of DAO activity can lead to the appearance of histamine intolerance, a clinical condition that may be treated by a low-histamine diet and oral DAO supplementation to enhance intestinal histamine degradation. As sources of DAO, porcine kidneys and certain legume seedlings are suitable components for the formulation of a DAO supplement. The aim of this work was to develop a rapid and reliable methodology for the in vitro determination of DAO activity in food matrices based on an enzymatic assay coupled to UHPLC-FL. The proposed method showed a satisfactory linearity and sensitivity and provided a relative standard deviation lower than 3%, guaranteeing method precision, and a mean recovery greater than 99% both for lyophilized pea sprouts and porcine kidney protein extracts. A high specificity is a key attribute of this method due to the use of histamine as the reaction substrate and the direct quantification of its degradation. Moreover, the lack of interference of catalase and hydrogen peroxide is another advantage in comparison with previously published methods. Lyophilized pea sprouts showed the greatest histamine-degrading activity (0.40 ± 0.01 mU/mg), followed by porcine kidney protein extracts (0.23 ± 0.01 mU/mg) and commercial DAO supplements (0.09 ± 0.06 mU/mg). This technique could be used as a tool to validate the DAO activity of food matrices of potential interest for the treatment of histamine intolerance.
1618-2642
http://hdl.handle.net/2445/163038
696790
In vitro determination of diamine oxidase activity in food matrices by an enzymatic assay coupled to UHPLC-FL.
oai:diposit.ub.edu:2445/1630412024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Muñoz-Esparza, Nelly
author
Latorre Moratalla, Mariluz
author
Comas Basté, Oriol
author
Toro Funes, Natalia
author
Veciana Nogués, María Teresa
author
Vidal Carou, Ma. Carmen
author
2019-07-11
The polyamines spermine, spermidine, and putrescine are involved in various biological processes, notably in cell proliferation and differentiation, and also have antioxidant properties. Dietary polyamines have important implications in human health, mainly in the intestinal maturation and in the differentiation and development of immune system. The antioxidant and anti-inflammatory effect of polyamine can also play an important role in the prevention of chronic diseases such as cardiovascular diseases. In addition to endogenous synthesis, food is an important source of polyamines. Although there are no recommendations for polyamine daily intake, it is known that in stages of rapid cell growth (i.e., in the neonatal period), polyamine requirements are high. Additionally, de novo synthesis of polyamines tends to decrease with age, which is why their dietary sources acquire a greater importance in an aging population. Polyamine daily intake differs among to the available estimations, probably due to different dietary patterns and methodologies of data collection. Polyamines can be found in all types of foods in a wide range of concentrations. Spermidine and spermine are naturally present in food whereas putrescine could also have a microbial origin. The main polyamine in plant-based products is spermidine, whereas spermine content is generally higher in animal-derived foods. This article reviews the main implications of polyamines for human health, as well as their content in food and breast milk and infant formula. In addition, the estimated levels of polyamines intake in different populations are provided.
2296-861X
http://hdl.handle.net/2445/163041
691959
31355206
Polyamines in Food
oai:diposit.ub.edu:2445/1630632024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Comas Basté, Oriol
author
Latorre Moratalla, Mariluz
author
Rabell-González, Judit
author
Veciana Nogués, María Teresa
author
Vidal Carou, Ma. Carmen
author
2020-02-22
Diamine oxidase (DAO) is one of the key enzymes involved in the degradation of dietary histamine. An imbalance of histamine scavenging systems leads to histamine intolerance, a diet-related disorder that may be tackled by following a low-histamine diet. Recently, the supplementation with exogenous DAO enzyme of animal origin has received the green light as a novel food to enhance intestinal degradation of histamine. This work performed a screening for histamine-degrading capacity of Leguminosae species in order to explore its potential suitability as plant-derived active ingredient of enzymatic supplements. In vitro DAO activity was determined both in raw pulses and lyophilised sprouts by an enzymatic assay coupled to UHPLC-FLD and several germination and storage conditions were assessed. The sprouts of edible legumes showed an in vitro histamine-degrading capacity ranging from 36.0 to 408.3 mU g-1, much higher than that found for the non-germinated seeds (0.14 - 1.95 mU g-1). The germination of legume seeds for 6 days in darkness provided the maximum DAO activity. Only the freezing storage of the lyophilized sprouts kept the enzymatic activity intact for at least 12 months. These results demonstrate that certain edible legumes could be suitable for the formulation of DAO supplements for the treatment of histamine intolerance.
0023-6438
http://hdl.handle.net/2445/163063
699701
Lyophilised legume sprouts as a functional ingredient for diamine oxidase enzyme supplementation in histamine intolerance
oai:diposit.ub.edu:2445/1635972024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Llorach, Rafael
author
Favari, Claudia
author
Alonso, David
author
Garcia Aloy, Mar
author
Andrés Lacueva, Ma. Cristina
author
Urpí Sardà, Mireia
author
2019-09-11
Legumes are a well-known source of phytochemicals and are commonly believed to have similar composition between different genera. To date, there are no studies evaluating changes in legumes to discover those compounds that help to discriminate for food quality and authenticity. The aim of this work was to characterize and make a comparative analysis of the composition of bioactive compounds between Cicer arietinum L. (chickpea), Lens culinaris L. (lentil) and Phaseolus vulgaris L. (white bean) through an LC-MS-Orbitrap metabolomic approach to establish which compounds discriminate between the three studied legumes. Untargeted metabolomic analysis was carried out by LC-MS-Orbitrap from extracts of freeze-dried legumes prepared from pre-cooked canned legumes. The metabolomic data treatment and statistical analysis were realized by using MAIT R's package, and final identification and characterization was done using MSn experiments. Fold-change evaluation was made through Metaboanalyst 4.0. Results showed 43 identified and characterized compounds displaying differences between the three legumes. Polyphenols, mainly flavonol and flavanol compounds, were the main group with 30 identified compounds, followed by α-galactosides (n = 5). Fatty acyls, prenol lipids, a nucleoside and organic compounds were also characterized. The fold-change analysis showed flavanols as the wider class of discriminative compounds of lentils compared to the other legumes; prenol lipids and eucomic acids were the most discriminative compounds of beans versus other legumes and several phenolic acids (such as primeveroside salycilic), kaempferol derivatives, coumesterol and α-galactosides were the most discriminative compounds of chickpeas. This study highlights the applicability of metabolomics for evaluating which are the characteristic compounds of the different legumes. In addition, it describes the future application of metabolomics as tool for the quality control of foods and authentication of different kinds of legumes.
0963-9969
http://hdl.handle.net/2445/163597
694889
Comparative metabolitefingerprinting of legumes using LC-MS-baseduntargeted metabolomics
oai:diposit.ub.edu:2445/1636702024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Selma, María Victoria
author
González-Sarrías, Antonio
author
Salas Salvadó, Jordi
author
Andrés Lacueva, Ma. Cristina
author
Alasalvar, Cesarettin
author
Örem, Asim
author
Tomas-Barberan, F. A. (Francisco A.)
author
Espín, Juan Carlos
author
2017-03-16
Background & aims: Urolithins are microbial metabolites produced after consumption of ellagitannincontaining foods such as pomegranates and walnuts. Parallel to isoflavone-metabolizing phenotypes, ellagitannin-metabolizing phenotypes (urolithin metabotypes A, B and 0; UM-A, UM-B and UM-0, respectively) can vary among individuals depending on their body mass index (BMI), but correlations between urolithin metabotypes (UMs) and cardiometabolic risk (CMR) factors are unexplored. We investigated the association between UMs and CMR factors in individuals with different BMI and health status. Methods: UM was identified using UPLC-ESI-qToF-MS in individuals consuming pomegranate or nuts. The associations between basal CMR factors and the urine urolithin metabolomic signature were explored in 20 healthy normoweight individuals consuming walnuts (30 g/d), 49 healthy overweightobese individuals ingesting pomegranate extract (450 mg/d) and 25 metabolic syndrome (MetS) patients consuming nuts (15 g-walnuts, 7.5 g-hazelnuts and 7.5 g-almonds/d). Results: Correlations between CMR factors and urolithins were found in overweight-obese individuals. Urolithin-A (mostly present in UM-A) was positively correlated with apolipoprotein A-I (P 0.05) and intermediate-HDL-cholesterol (P 0.05) while urolithin-B and isourolithin-A (characteristic from UM-B) were positively correlated with total-cholesterol, LDL-cholesterol (P 0.001), apolipoprotein B (P 0.01), VLDL-cholesterol, IDL-cholesterol, oxidized-LDL and apolipoprotein B:apolipoprotein A-I ratio (P 0.05). In MetS patients, urolithin-A only correlated inversely with glucose (P 0.05). Statin-treated MetS patients with UM-A showed a lipid profile similar to that of healthy normoweight individuals while a poor response to lipid-lowering therapy was observed in MB patients. Conclusions: UMs are potential CMR biomarkers. Overweight-obese individuals with UM-B are at increased risk of cardiometabolic disease, whereas urolithin-A production could protect against CMR factors. Further research is warranted to explore these associations in larger cohorts and whether the effect of lipidlowering drugs or ellagitannin-consumption on CMR biomarkers depends on individuals' UM.
0261-5614
http://hdl.handle.net/2445/163670
671159
The gut microbiota metabolism of pomegranate or walnut ellagitannins yields two urolithin-metabotypes that correlate with cardiometabolic risk biomarkers: Comparison between normoweight, overweight-obesity and metabolic syndrome.
oai:diposit.ub.edu:2445/1637032024-03-12T17:02:13Zcom_2445_7201com_2445_7181com_2445_7209com_2445_7521com_2445_96580col_2445_7202col_2445_7210col_2445_7522col_2445_108487
00925njm 22002777a 4500
dc
Almanza Aguilera, Enrique
author
Urpí Sardà, Mireia
author
Llorach, Rafael
author
Vázquez Fresno, Rosa
author
Garcia Aloy, Mar
author
Carmona Pontaque, Francesc
author
Sànchez, Àlex (Sànchez Pla)
author
Madrid Gambín, Francisco Javier
author
Estruch Riba, Ramon
author
Corella Piquer, Dolores
author
Andrés Lacueva, Ma. Cristina
author
2017-06-07
The study of biomarkers of dietary patterns including the Mediterranean diet (MedDiet) is scarce and could improve the assessment of these patterns. Moreover, it could provide a better understanding of health benefits of dietary patterns in nutritional epidemiology. We aimed to determine a robust and accurate biomarker associated with a high adherence to a MedDiet pattern that included dietary assessment and its biological effect. In this cross-sectional study, we included 56 and 63 individuals with high (H-MDA) and low (L-MDA) MedDiet adherence categories, respectively, all from the Prevención con Dieta Mediterránea trial. A 1H-NMR-based untargeted metabolomics approach was applied to urine samples. Multivariate statistical analyses were conducted to determine the metabolite differences between groups. A stepwise logistic regression and receiver operating characteristic curves were used to build and evaluate the prediction model for H-MDA. Thirty-four metabolites were identified as discriminant between H-MDA and L-MDA. The fingerprint associated with H-MDA included higher excretion of proline betaine and phenylacetylglutamine, among others, and decreased amounts of metabolites related to glucose metabolism. Three microbial metabolites - phenylacetylglutamine, p-cresol and 4-hydroxyphenylacetate - were included in the prediction model of H-MDA (95% specificity, 95% sensitivity and 97% area under the curve). The model composed of microbial metabolites was the biomarker that defined high adherence to a Mediterranean dietary pattern. The overall metabolite profiling identified reflects the metabolic modulation produced by H-MDA. The proposed biomarker may be a better tool for assessing and aiding nutritional epidemiology in future associations between H-MDA and the prevention or amelioration of chronic diseases.
0955-2863
http://hdl.handle.net/2445/163703
672404
Microbial metabolites are associated with a high adherence to a Mediterranean dietary pattern using a 1h-nmr-based untargeted metabolomics approach
oai:diposit.ub.edu:2445/1640572024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Khymenets, Olha
author
Rabassa Bonet, Montserrat
author
Rodríguez-Palmero Seuma, María
author
Rivero Urgell, M. Montserrat
author
Urpí Sardà, Mireia
author
Tulipani, Sara
author
Brandi, P.
author
Campoy Folgoso, Cristina
author
Santos-Buelga, Celestino
author
Andrés Lacueva, Ma. Cristina
author
2016-07-06
Polyphenols play an important role in human health. To address their accessibility to a breastfed infant, we planned to evaluate whether breast milk (BM) (colostrum, transitional, and mature) epicatechin metabolites could be related to the dietary habits of mothers. The polyphenol consumption of breastfeeding mothers was estimated using a food frequency questionnaire and 24 h recalls. Solid-phase extraction-ultra performance liquid chromatography-tandem mass spectrometry (SPE-UPLC-MS/MS) was applied for direct epicatechin metabolite analysis. Their bioavailability in BM as a result of dietary ingestion was confirmed in a preliminary experiment with a single dose of dark chocolate. Several host and microbial phase II metabolites of epicatechin were detected in BM among free-living lactating mothers. Interestingly, a modest correlation between dihydroxyvalerolactone sulfate and the intake of cocoa products was observed. Although a very low percentage of dietary polyphenols is excreted in BM, they are definitely in the diet of breastfed infants. Therefore, evaluation of their role in infant health could be further promoted.
0021-8561
http://hdl.handle.net/2445/164057
663197
Dietary Epicatechin Is Available to Breastfed Infants through Human Breast Milk in the Form of Host and Microbial Metabolites
oai:diposit.ub.edu:2445/1640592024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Khymenets, Olha
author
Crespo, M. Carmen
author
Dangles, Olivier
author
Rakotomanomana, N.
author
Andrés Lacueva, Ma. Cristina
author
Visioli, F. (Francesco)
author
2016-03-31
Among the various (poly)phenols that are being sold as such or as part of a more complex mixture, hydroxytyrosol (HT) is the only one that bears a European Food Safety Authority health claim. Therefore, several HT-based products are being developed and sold and it becomes necessary to evaluate its accessibility following ingestion. Twenty-one volunteers were recruited for a randomized, crossover, placebo-controlled, and double-blind intervention study.We performed a Latin square design: after one-week washout, i.e. olive-free diet, subjects were randomly assigned to the placebo (maltodextrin), 5, or 25 mg/day HT group. Twenty-four hour urine samples were collected after the intervention week, and baseline urines were collected the week before the study and during periods of washout. The results show that HT given as the foremost component of a nutraceutical preparation is bioavailable and is recovered in the urine chiefly as sulphate-3′.
1756-4646
http://hdl.handle.net/2445/164059
659165
Human hydroxytyrosol's absorption and excretion from a nutraceutical
oai:diposit.ub.edu:2445/1640882024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Urpí Sardà, Mireia
author
Andrés Lacueva, Ma. Cristina
author
Rabassa Bonet, Montserrat
author
Ruggiero, Carmelinda
author
Zamora-Ros, Raul
author
Bandinelli, Stefania
author
Ferrucci, Luigi
author
Cherubini, Antonio
author
2015-09
Background. Frailty, an age-related state of increased vulnerability, is associated with a higher risk of multiple adverse events. Studies have suggested that the quality of dietary intake may affect the development of frailty. We hypothesized that frailty in older subjects would be associated with dietary total polyphenols (DTP) intake and its biomarker, urinary total polyphenols (UTP). Methods. The Invecchiare in Chianti (InCHIANTI) Study is a prospective cohort study set in the Chianti area (Italy). We used data at baseline from 811 participants aged 65 years and older. UTP was determined using the Folin-Ciocalteu assay after solid-phase extraction. DTP was estimated using a validated Food Frequency Questionnaire and our own polyphenol database. The frailty, prefrailty, and nonfrailty states were defined according to the Fried and colleagues' criteria. Multinomial logistic regressions adjusted for potential confounders were used to assess the relationship between polyphenols and frailty. Results. Both DTP and UTP concentrations progressively decrease from nonfrail to frail participants. Participants in the highest UTP tertile compared to those in the lowest tertile were significantly less likely to be both frail (odds ratio [OR] = 0.36 [0.14-0.88], p = .025) and prefrail (OR = 0.64 [0.42-0.98], p = .038). Exhaustion and slowness were the only individual frailty criteria significantly associated with UTP tertiles. No significant association was observed between frailty and DTP, after adjustment for covariates. Conclusions. High concentrations of UTP were associated with lower prevalence of frailty and prefrailty in an older community-dwelling population. A polyphenol-rich diet may protect against frailty in older persons. Our findings should be confirmed in longitudinal studies.
1079-5006
http://hdl.handle.net/2445/164088
662109
The relationship between urinary total polyphenols and the frailty phenotype in a community-dwelling older population: the InCHIANTI study.
oai:diposit.ub.edu:2445/1643742024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580com_2445_16685col_2445_7210col_2445_108487col_2445_16686
00925njm 22002777a 4500
dc
Magrinyà Navarro, Núria
author
Bou Novensà, Ricard
author
Rius Bofill, Núria
author
Codony Salcedo, Rafael
author
Guardiola Ibarz, Francesc
author
2016-04
This research evaluates the effects of adding a tocopherol mix (200 mg/kg), two nitrite sources (sodium nitrite or a nitrate-rich vegetable concentrate) and the use of Staphylococcus carnosus together with three fermentation types that varied in temperature (12 h at 4 ºC or 16 ºC) on different quality parameters and acceptability of cooked cured sausages after vacuum packing and storage at 4 ºC for 120 days. In the presence of S. carnosus, residual nitrate and nitrite levels were reduced. Sausages containing vegetable concentrates and without S. carnosus resulted in higher amounts of residual nitrate and lower curing efficiency. The lowest values in redness and acceptability were observed in those sausages without starter cultures. The addition of tocopherols had no effect on oxidative status and susceptibility to oxidation. However, the highest amount of hydroperoxides was related with nitrite decreased formation. Overall, vegetable concentrates can be used as curing agents if fermentation with a nitrate-reducing starter culture is allowed.
1082-0132
http://hdl.handle.net/2445/164374
651791
Use of tocopherol extract and different nitrite sources and starter cultures in the production of organic botifarra catalana, a cooked cured sausage
oai:diposit.ub.edu:2445/1643762024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Alemán, Mercedes
author
Bou Novensà, Ricard
author
Tres Oliver, Alba
author
Polo, Javier
author
Codony Salcedo, Rafael
author
Guardiola Ibarz, Francesc
author
2016-04-01
Fortification of food products with iron is a common strategy to prevent or overcome iron deficiency. However, any iron form is a pro-oxidant and its addition will cause off-flavours and reduce product's shelf life. A highly bioavailable heme iron ingredient was selected to fortify a chocolate cream used to fill sandwich-type cookies. Two different strategies were assessed for avoiding the heme iron catalytic effect on lipid oxidation: ascorbyl palmitate addition and co-spray-drying of heme iron with calcium caseinate. Oxidation development and sensory acceptability were monitored in the cookies over one-year of storage at room temperature in the dark. The addition of ascorbyl palmitate protected from oxidation and from tocopherols and tocotrienols loss during cookies preparation. In general, ascorbyl palmitate, either alone or in combination with the co-spray-dried heme iron, prevented primary oxidation and hexanal formation during storage. The combination of both strategies resulted in cookies that were sensory acceptable after 1 year of storage.
0308-8146
http://hdl.handle.net/2445/164376
654608
Oxidative stability of a heme iron-fortified bakery product: Effectiveness of ascorbyl palmitate and co-spray-drying of heme iron with calcium caseinate
oai:diposit.ub.edu:2445/1644332024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7198col_2445_108487col_2445_7199
00925njm 22002777a 4500
dc
Ruiz-Iglesias, Patricia
author
Estruel Amades, Seila
author
Camps i Bossacoma, Mariona
author
Massot Cladera, Malen
author
Franch i Masferrer, Àngels
author
Pérez-Cano, Francisco J.
author
Castell, Margarida
author
2020-05-01
Intensive training and exhausting exercise can disrupt innate and acquired immunity. The flavanone hesperidin has shown immunomodulatory properties in physiological and some pathological conditions, and positive e ects on exercise-induced oxidative stress. Nevertheless, it remains uncertain whether it also prevents exhausting exercise-induced immune alterations. The aim of this study was to establish the e ect of oral hesperidin supplementation on the systemic immune system in rats following an intensive training and exhausting exercise. For this purpose, female Wistar rats were randomized into an intensive training group or a sedentary group. Intensive training was induced by running in a treadmill 5 days per week (including two exhausting tests) for five weeks. Throughout the training period, 200 mg/kg of hesperidin or vehicle was administered by oral gavage three times per week. At the end, blood, thymus, spleen and macrophages were collected before, immediately after and 24 h after an additional final exhaustion test. Hesperidin supplementation enhanced natural killer cell cytotoxicity and the proportion of phagocytic monocytes, attenuated the secretion of cytokines by stimulated macrophages, prevented the leukocytosis induced by exhaustion and increased the proportion of T helper cells in the thymus, blood and spleen. These results suggest that hesperidin can prevent exhausting exercise-induced immune alteration.
2072-6643
http://hdl.handle.net/2445/164433
700823
32369998
Influence of hesperidin on systemic immunity of rats following an intensive training and exhausting exercise
oai:diposit.ub.edu:2445/1647412024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Rodríguez Sánchez, Raquel
author
Tres Oliver, Alba
author
Sala, Roser
author
Garcés-Narro, Carlos
author
Guardiola Ibarz, Francesc
author
Gasa, J.
author
Barroeta, A.C.
author
2019-10-01
The aim of the present study is to assess the effect of the dietary free fatty acid (FFA) content and dietary fat saturation degree on the fatty-acid (FA) digestibility and lipid-class content along the gastrointestinal tract and excreta in broiler chickens. The 8 experimental diets resulted from replacing crude soybean oil with soybean acid oil from chemical refining, or crude palm oil with palm FA distillate from physical refining. Thus, there were 4 soybean and 4 palm diets with 6% added fat varying in their FFA% (5, 15, 35, and 50%). Samples of digestive content (gizzard, duodenum, jejunum, and ileum) and excreta were collected at 14 D for the determination of the FA digestibility and lipid-class content. The total FA digestibility coefficients reported for the chickens fed S diets in the jejunum, ileum, and excreta were higher than for those fed P diets (P ≤ 0.02). The general greater digestibility of the unsaturated diets was mainly explained by a higher contribution of the ileum to the absorption of saturated FA. The dietary FFA content mainly affected the FA absorption process. The diets with 50% FFA presented lower saturated FA digestibility coefficients in the jejunum and ileum (P ≤ 0.03), and higher content of FFA in the ileum and excreta (P ≤ 0.014), in comparison to the diets with 5% FFA. The 15% FFA diets were not different from the 5% FFA diets, regarding the saturated FA digestibility in the jejunum and excreta, and the FFA content in the ileum and excreta. It was concluded that unsaturated diets with moderate content of dietary FFA (up to 15%) could be used in broiler-chicken starter diets, as they led to similar FA absorption and performance results to the diets with the lowest dietary FFA content. From the present study, it has also been concluded that dietary saturated FA content has a greater impact on FA absorption than the dietary FFA content has.
0032-5791
http://hdl.handle.net/2445/164741
690507
Effects of dietary free fatty-acid content and saturation degree on lipid-class composition and fatty-acid digestibility along the gastrointestinal tract in broiler starter chickens
oai:diposit.ub.edu:2445/1647462024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Rodríguez Sánchez, Raquel
author
Tres Oliver, Alba
author
Sala, Roser
author
Guardiola Ibarz, Francesc
author
Barroeta, A.C.
author
2019-03-01
The aim of the present study is to evaluate the effect of the dietary fat saturation degree and age on the lipid class (TAG, DAG, MAG, and FFA) composition and fatty acid digestibility along the gastrointestinal tract (GIT) and excreta in broiler chickens. A total of 120 one-day-old female broiler chickens were randomly distributed in 2 dietary treatments (6 cages/treatment), which resulted from the supplementation of a basal diet with 6% of soybean oil or palm oil. Two digestibility balances were carried out at 14 and 35 d and fatty acid digestibility and lipid class composition were determined in the gizzard, duodenum, jejunum, ileum, and excreta. Along de GIT, both fatty acid digestibility and lipid class composition were influenced by the dietary fat source and the age of the chickens. The absorption of the unsaturated fat was more efficient and faster than it was for the saturated fat. The ability of adult chickens to absorb fat was higher than for young chickens. The results show that the duodenum is the main place of fat digestion (hydrolysis), and the jejunum the main place of fat absorption. The role of the ileum on fat absorption is very important, as it is the last segment of the GIT where the absorption of fatty acids has been described. Thus, it was the contribution of the ileum that was responsible for the higher fat utilization observed for animals fed the unsaturated diet than for those fed the saturated diet at 14 d, and it was also responsible for the improvement on the utilization of the saturated diet between 14 and 35 d. All the results suggest that the absorption of fatty acids is more limiting than is hydrolysis, because the main differences were observed in the jejunum and ileum, where the absorption of fatty acids takes place.
0032-5791
http://hdl.handle.net/2445/164746
682505
Evolution of lipid classes and fatty acid digestibility along the gastrointestinal tract of broiler chickens fed different fat sources at different ages
oai:diposit.ub.edu:2445/1651512024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209com_2445_7203com_2445_107205com_2445_13750col_2445_108487col_2445_7210col_2445_7204col_2445_107206col_2445_13751
00925njm 22002777a 4500
dc
Pérez Martí, Albert
author
Garcia-Guasch, Maite
author
Tresserra i Rimbau, Anna
author
Carrilho do Rosário, Alexandra
author
Estruch Riba, Ramon
author
Salas Salvadó, Jordi
author
Martínez-González, Miguel Ángel, 1957-
author
Lamuela Raventós, Rosa Ma.
author
Marrero González, Pedro F.
author
Haro Bautista, Diego
author
Relat Pardo, Joana
author
2017-08
Scope: Fibroblast growth factor 21 (FGF21) is considered a promising therapeutic candidate for the treatment of obesity. Since FGF21 production is regulated by various nutritional factors, we analyze the impact of low protein intake on circulating levels of this growth hormone in mice and in a sub cohort of the PREDIMED (Prevención con Dieta Mediterránea) trial. We also describe the role of hepatic FGF21 in metabolic adaptation to a low-protein diet (LPD). Methods and results: We fed control and liver-specific Fgf21 knockout (LFgf21KO) mice a LPD. This diet increased FGF21 production by inducing its overexpression in liver, and this correlated with a body weight decrease without changes in food intake. The LPD also caused FGF21-dependent browning in subcutaneous white adipose tissue (scWAT), as indicated by an increase in the expression of uncoupling protein 1 (UCP1). In a subgroup of 78 individuals from the PREDIMED trial, we observed an inverse correlation between protein intake and circulating FGF21 levels. Conclusions: Our results reinforce the involvement of FGF21 in coordinating energy homeostasis under a range of nutritional conditions. Moreover, here we describe an approach to increase the endogenous production of FGF21, which if demonstrated functional in humans, could generate a treatment for obesity.
1613-4125
http://hdl.handle.net/2445/165151
668644
A low-protein diet induces body weight loss and browning of subcutaneous white adipose tissue through enhanced expression of hepatic Fibroblast Growth Factor 21 (FGF21).
oai:diposit.ub.edu:2445/1652822024-03-12T17:02:13Zcom_2445_7198com_2445_7181com_2445_96580col_2445_7199col_2445_108487
00925njm 22002777a 4500
dc
Grases Pintó, Blanca
author
Torres-Castro, Paulina
author
Abril Gil, Maria del Mar
author
Castell, Margarida
author
Rodríguez Lagunas, María José
author
Pérez-Cano, Francisco J.
author
Franch i Masferrer, Àngels
author
2019-05-01
Neonates are born with an immature immune system, which develops during the first stages of life. This early immaturity is more acute in preterm newborns. The aim of the present study was to set up a preterm rat model, in which representative biomarkers of innate and adaptive immunity maturation that could be promoted by certain dietary interventions are established. Throughout the study, the body weight was registered. To evaluate the functionality of the intestinal epithelial barrier, in vivo permeability to dextrans was measured and a histomorphometric study was performed. Furthermore, the blood cell count, phagocytic activity of blood leukocytes and plasmatic immunoglobulins (Ig) were determined. Preterm rats showed lower erythrocyte and platelet concentration but a higher count of leukocytes than the term rats. Although there were no changes in the granulocytes' ability to phagocytize, preterm monocytes had lower phagocytic activity. Moreover, lower plasma IgG and IgM concentrations were detected in preterm rats compared to full-term rats, without affecting IgA. Finally, the intestinal study revealed lower permeability in preterm rats and reduced goblet cell size. Here, we characterized a premature rat model, with differential immune system biomarkers, as a useful tool for immunonutritional studies aimed at boosting the development of the immune system.
2072-6643
http://hdl.handle.net/2445/165282
691286
31052461
A Preterm Rat Model for Immunonutritional Studies
oai:diposit.ub.edu:2445/1655172024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Crous-Masó, Joan
author
Palomeras, Sonia
author
Relat Pardo, Joana
author
Camó, Cristina
author
Martínez Garza, Úrsula
author
Planas, Marta
author
Feliu, Lidia
author
Puig i Miquel, Teresa
author
2018-05-11
()-Epigallocatechin 3-gallate (EGCG) is a natural polyphenol from green tea with reported anticancer activity and capacity to inhibit the lipogenic enzyme fatty acid synthase (FASN), which is overexpressed in several human carcinomas. To improve the pharmacological profile of EGCG, we previously developed a family of EGCG derivatives and the lead compounds G28, G37 and G56 were characterized in HER2-positive breast cancer cells overexpressing FASN. Here, diesters G28, G37 and G56 and two G28 derivatives, monoesters M1 and M2, were synthesized and assessed in vitro for their cytotoxic, FASN inhibition and apoptotic activities in MDA-MB-231 triple-negative breast cancer (TNBC) cells. All compounds displayed moderate to high cytotoxicity and significantly blocked FASN activity, monoesters M1 and M2 being more potent inhibitors than diesters. Interestingly, G28, M1, and M2 also diminished FASN protein expression levels, but only monoesters M1 and M2 induced apoptosis. Our results indicate that FASN inhibition by such polyphenolic compounds could be a new strategy in TNBC treatment, and highlight the potential anticancer activities of monoesters. Thus, G28, G37, G56, and most importantly M1 and M2, are anticancer candidates (alone or in combination) to be further characterized in vitro and in vivo.
1420-3049
http://hdl.handle.net/2445/165517
680419
29751678
(-)-Epigallocatechin 3-Gallate Synthetic Analogues Inhibit Fatty Acid Synthase and Show Anticancer Activity in Triple Negative Breast Cancer.
oai:diposit.ub.edu:2445/1655442024-03-12T17:02:13Zcom_2445_7198com_2445_7181com_2445_96580col_2445_7199col_2445_108487
00925njm 22002777a 4500
dc
Martín-Peláez, Sandra
author
Camps i Bossacoma, Mariona
author
Massot Cladera, Malen
author
Rigo Adrover, Maria del Mar
author
Franch i Masferrer, Àngels
author
Pérez-Cano, Francisco J.
author
Castell, Margarida
author
2017-10
SCOPE: To establish the role of cocoa theobromine on gut microbiota composition and fermentation products after cocoa consumption in rats. METHODS AND RESULTS: Lewis rats were fed either a standard diet (RF diet), a diet containing 10% cocoa (CC diet) or a diet including 0.25% theobromine (TB diet) for 15 days. Gut microbiota (fluorescence in situ hybridization coupled to flow cytometry and metagenomics analysis), SCFA and IgA-coated bacteria were analyzed in fecal samples. CC and TB diets induced lower counts of E. coli whereas TB diet led to lower counts of Bifidobacterium spp., Streptococcus spp. and Clostridium histolyticum-C. perfingens group compared to RF diet. Metagenomics analysis also revealed a different microbiota pattern among the studied groups. The SCFA content was higher after both CC and TB diets, which was mainly due to enhanced butyric acid production. Furthermore, both diets decreased the proportion of IgA-coated bacteria. CONCLUSION: Cocoa's theobromine plays a relevant role in some effects related to cocoa intake, such as the lower proportion of IgA-coated bacteria. Moreover, theobromine modifies gut microbiota although other cocoa compounds could also act on intestinal bacteria, attenuating or enhancing the theobromine effects.
1613-4125
http://hdl.handle.net/2445/165544
674378
Effect of cocoa's theobromine on intestinal microbiota of rats
oai:diposit.ub.edu:2445/1665382024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Rodriguez Cuenca, Sergio
author
Carobbio, Stefania
author
Barceló-Coblijn, Gwendolyn
author
Prieur, Xavier
author
Relat Pardo, Joana
author
Amat, Ramon
author
Campbell, Mark
author
Dias, Ana Rita
author
Bahri, Myriam
author
Gray, Sarah L.
author
Vidal-Puig, Antonio
author
2018-05-27
Familial partial lipodystrophic syndrome 3 (FPLD3) is associated with mutations in the transcription factor PPAR. The P467L mutant confers a dominant negative effect. We have previously investigated the pathophysiology of FPLD3 using a humanised mouse harbouring an equivalent mutation (P465L) in PPAR that recapitulated the human clinical phenotype. One of the key clinical manifestations observed in humans and mice is the accumulation of fat in the liver. Here, we dissect the molecular mechanisms that facilitate accumulation of lipids in the liver and characterise the negative effect of the PPAR mutation on the activation of PPAR in vivo by fibrates. P465L mice have increased insulin and FFAs, decreased secretion of VLDL when fed HFD and impaired hypolipidemic response to WY14643. Thus, the phenotype of PPAR mutations may synergise with defects on PPAR function, indicating that the phenotype of the FPLD3 patients may not only be attributed to the dysfunction of PPAR.
1462-8902
http://hdl.handle.net/2445/166538
680475
29790245
P465L pparγ mutation confers partial resistance to the hypolipidemic action of fibrates.
oai:diposit.ub.edu:2445/1665422024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Garza Puentes, Andrea de la
author
Martí Alemany, Adrià
author
Chisaguano Tonato, Aida Maribel
author
Montes Goyanes, Rosa
author
Castellote Bargalló, Ana Isabel
author
Torres Espinola, Francisco José
author
García-Valdés, Luz
author
Escudero-Marín, Mireia
author
Segura Moreno, Maite
author
Campoy, Cristina
author
López Sabater, María del Carmen
author
2019-09-09
This study analyzed how maternal obesity a ected fatty acids (FAs) in breast milk and their association with infant growth and cognition to raise awareness about the programming e ect of maternal health and to promote a healthy prenatal weight. Mother-child pairs (n = 78) were grouped per maternal pre-pregnancy body mass index (BMI): normal-weight (BMI = 18.5-24.99), overweight (BMI = 25-29.99) and obese (BMI > 30). Colostrum and mature milk FAs were determined. Infant anthropometry at 6, 18 and 36 months of age and cognition at 18 were analyzed. Mature milk exhibited lower arachidonic acid (AA) and docosahexaenoic acid (DHA), among others, than colostrum. Breast milk of non-normal weight mothers presented increased saturated FAs and n6:n3 ratio and decreased -linolenic acid (ALA), DHA and monounsaturated FAs. Infant BMI-for-age at 6 months of age was inversely associated with colostrum n6 (e.g., AA) and n3 (e.g., DHA) FAs and positively associated with n6:n3 ratio. Depending on the maternal weight, infant cognition was positively influenced by breast milk linoleic acid, n6 PUFAs, ALA, DHA and n3 LC-PUFAs, and negatively a ected by n6:n3 ratio. In conclusion, this study shows that maternal pre-pregnancy BMI can influence breast milk FAs and infant growth and cognition, endorsing the importance of a healthy weight in future generations.
2072-6643
http://hdl.handle.net/2445/166542
697012
31505767
The effect of maternal obesity on breast milk fatty acids and its association with infant growth and cognition-The Preobe follow-up
oai:diposit.ub.edu:2445/1665992024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Vichi, S. (Stefania)
author
Cortés Francisco, Nuria
author
Caixach Gamisans, Josep
author
Barrios, Gonçal
author
Mateu, Jordi
author
Ninot, Antònia
author
Romero, Agustí
author
2016-07-27
The epicuticular wax (EW) layer is located on the surface of most of plants organs. It provides the cuticle with most of its properties and is the primary barrier against biotic and abiotic stress. Despite the importance of Olea europaea cultivation, few studies have characterized the EW covering leaves and olives, which could be involved in resistance to both infection and environmental conditions. In the present study, wide-ranging screening was carried out using direct-injection electrospray ionisation coupled to high-resolution mass spectrometry to analyse EW in developing olives of nine varieties. The proportions of EW fractions (wax esters (WEs), diacylglycerols, triacylglycerols (TAGs), triterpenic acids and aldehydes) strongly depended on the olive cultivar, and in only a few cases were they influenced by the sampling date. The specific compositions of the major fractions, WEs and TAGs, were strictly related to the cultivar, while the degree of unsaturation and the chain length of the WEs evolved throughout the four weeks prior to the olive colour turning.
0021-8561
http://hdl.handle.net/2445/166599
669782
Epicuticular Wax in Developing Olives (Olea europaea) Is Highly Dependent upon Cultivar and Fruit Ripeness
oai:diposit.ub.edu:2445/1666192024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Hachicha Hbaieb, Rim
author
Kotti, Faten
author
Cortés Francisco, Nuria
author
Caixach Gamisans, Josep
author
Gargouri, Mohamed
author
Vichi, S. (Stefania)
author
2016-05-06
Several factors affect virgin olive oil (VOO) phenolic profile. The aim of this study was to monitor olive hydrolytic (β-glucosidase) and oxidative (POX and PPO) enzymes during olive ripening and storage and to determine their capacity to shape VOO phenolic profile. To this end, olives from the cultivars Chétoui and Arbequina were stored at 4 °C or 25 °C for 4 weeks and their enzymatic activities and oil phenolic profiles were compared to those of ripening olives. We observed different trends in enzymes activities according to cultivar and storage temperature. Secoiridoid compounds, determined by HRMS, and their deacetoxylated, oxygenated, and deacetoxy-oxygenated derivatives were identified and their contents differed between the cultivars according to olive ripening degree and storage conditions. These differences could be due to β-glucosidase, POX and PPO activities changes during olive ripening and storage. Results also show that oxidised phenolic compounds could be a marker of VOO ''freshness'.
0308-8146
http://hdl.handle.net/2445/166619
669781
Ripening and storage conditions of Chétoui and Arbequina olives: Part II. Effect on olive endogenous enzymes and virgin olive oil secoiridoid profile determined by high resolution mass spectrometry
oai:diposit.ub.edu:2445/1666972024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Hachicha Hbaieb, Rim
author
Kotti, Faten
author
Vichi, S. (Stefania)
author
Gargouri, Mohamed
author
2017
The composition and biochemical characteristics of olive fruits, mainly olive enzymes system, are crucial in determining the final quality of virgin olive oil (VOO). Thus, olives endogenous olive enzyme activities were studied during Chétoui and Chemlali olive ripening. The compositional quality of the corresponding VOO was also studied. Peroxidase (POX) and β-glucosidase activities increased during olives ripening. However, polyphenoloxidase activity decreased slowly. Moreover,the POX enzyme appears to play an essential role in determining VOO total phenol amounts, as the decrease in phenol content registered during olive ripening coincided with the increase in POX activity. A positive correlation between oil antioxidant activity and the total phenol content was established for both the olive cultivars studied. With regard to pigments, chlorophyll content wasmuch higher than that of carotenoids in both Chétoui and Chemlali oils. Moreover, different trends in chlorophyll and carotenoid contents were observed, depending on the olive cultivar. Concerning volatile compounds, our results showed that the highest content of total C6, C5 LOX compounds and pentene dimers was observed at a RI of approximately 3 for both cultivars. However, C6 alcohols and total C5 compounds decreased in Chétoui and Chemlali oils, respectively, during olive ripening.
1438-7697
http://hdl.handle.net/2445/166697
669791
Evolution of endogenous enzyme activities and virgin olive oil characteristics during Chétoui and Chemlali olive ripening
oai:diposit.ub.edu:2445/1667042024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_20402col_2445_108487col_2445_20403
00925njm 22002777a 4500
dc
Barenys Espadaler, Marta
author
Molins Raich, Anna
author
Amorós-Galicia, Lola
author
Flick, Burkhard
author
Gómez Catalán, Jesús
author
2019-08-30
The inclusion of a read-out to detect functional consequences of craniofacial alterations in the zebrafish embryotoxicity test will allow to evaluate these alterations which are difficult to assess morphologically, and to detect alterations in cranial nerves functions leading to impairment of jaw movements. In this study we have established an ingestion test in zebrafish larvae younger than 120 hpf. To overcome the challenge of evaluating larvae which still do not present independent feeding behaviour, we have tested the ability of 72, 96 or 102 hpf larvae to ingest food mixed with fluorescent microspheres under several conditions (dark/light, with/without shaking) to find the best experimental set-up for the test. We have included the investigation of two substances as potential positive controls: ketoconazole and tricaine. Ketoconazole 10 μM exposure during development produced significant embryotoxic effects including a characteristic craniofacial alteration pattern consisting in impaired development of brain, nasal cavity, mouth opening and jaw, as well as a significant decrease in food intake. Tricaine exposure at 380 μM during the food availability period significantly decreased the food intake. The method proposed will be a useful alternative tool to animal testing to detect compounds inducing adverse effects on craniofacial development.
0887-2333
http://hdl.handle.net/2445/166704
692116
Implementation of a functional endpoint to the zebrafish embryotoxicity test to evaluate craniofacial abnormalities
oai:diposit.ub.edu:2445/1667222024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Tsimidou, Maria Z.
author
Nenadis, Nikolaos
author
Mastralexi, Aspasia
author
Servili, Maurizio
author
Butinar, Bojan
author
Vichi, S. (Stefania)
author
Winkelmann, Ole
author
García-González, Diego Luis
author
Gallina Toschi, Tullia
author
2019-07-02
Toward a harmonized and standardized procedure for the determination of total hydroxytyrosol and tyrosol content in virgin olive oil (VOO), the pros of a recently published in house validated ultra high performance liquid chromatography (UHPLC) protocol are discussed comparatively with those of other procedures that determine directly or indirectly the compounds hosted under the health claim on 'olive oil polyphenols' (EC regulation 432/2012). Authentic VOOs were analyzed with five di erent liquid chromatographic separation protocols and 1H-NMRone in five di erent laboratories with expertise in VOO phenol analysis within three months. Data comparison indicated di erences in absolute values. Method comparison using appropriate tools (Passing-Bablok regression and Bland Altman analyses) for all protocols vs. the UHPLC one indicated slight or statistically significant di erences. The results were also discussed in terms of cost e ectiveness, detection means, standard requirements and ways to calculate the total hydroxytyrosol and tyrosol content. Findings point out that the in-house validated fit for the purpose UHPLC protocol presents certain pros that should be exploited by the interested parties. These are the simplicity of sample preparation, fast elution time that increase the number of samples analyzed per day and integration of well-resolved peaks with the aid of only two commercially available external standards. Importance of correction factors in the calculations is stressed.
1420-3049
http://hdl.handle.net/2445/166722
698538
31269664
Toward a harmonized and standardized protocol for the determination of total hydroxytyrosol and tyrosol content in virgin olive oil (VOO). The pros of a fit for the purpose ultra high performance liquid chromatography (UHPLC) procedure
oai:diposit.ub.edu:2445/1667572024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_20402col_2445_108487col_2445_20403
00925njm 22002777a 4500
dc
Barenys Espadaler, Marta
author
Reverte, Ingrid
author
Masjosthusmann, Stefan
author
Gómez Catalán, Jesús
author
Fritsche, Ellen
author
2020-05
The increasing use of illegal drugs by pregnant women causes a public health concern because it is associated with health risks for mothers and their developing children. One of such drugs is MDMA (3,4-methylenedioxymethamphetamine) or ecstasy due to its high consumption in relevant age and sex groups and its adverse effects on human and rodent developing brains. To Journal Pre-proof 2 thoroughly review the current knowledge on the developmentally neurotoxic potential of MDMA we systematically collected and summarized articles investigating developmental neurotoxicity (DNT) of MDMA in humans and animals in in vivo and in vitro. In addition, we summarized the findings in a putative adverse outcome pathway (AOP). From an initial 299 articles retrieved from the bibliographic databases Web of Science, PubMed and DART, we selected 39 articles according to inclusion/exclusion criteria for data collection after title/abstract and full text screening. Of these 3 where epidemiological studies, 34 where in vivo studies in mice and rats and 2 were in vitro studies. The three epidemiological studies reported from the same longitudinal study and suggested that MDMA exposure during pregnancy impairs neuromotor function in infants. In rat, postnatal exposure towards MDMA also caused locomotor deficits as well as impaired spatial learning that might be associated with decreased serotonin levels in the hippocampus. In vitro MDMA caused cytotoxicity at high concentrations and effects on the serotonergic and neuritogenic alterations at lower concentrations which are in line with some of the in vivo alterations observed. Considering the adverse outcomes of developmental MDMA described in humans and in rodents we summarized the first putative AOP on developmental compound exposure leading to impaired neuromotor function in children. For generation of this AOP, MDMA exposure was taken as a model compound. In addition, we hypothesized a second AOP involving developmental disturbance of the dopaminergic system. However, further in vitro mechanistic studies are needed to understand the molecular initiating event(s) (MIE) triggering the downstream cascades and obtain consistent evidences causally linking the adverse outcome to effects at the cellular, organ and organism level.
0161-813X
http://hdl.handle.net/2445/166757
693572
Developmental neurotoxicity of MDMA. A systematic literature review summarized in a putative adverse outcome pathway
oai:diposit.ub.edu:2445/1667582024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7198col_2445_108487col_2445_7199
00925njm 22002777a 4500
dc
Abril Gil, Maria del Mar
author
Pérez-Cano, Francisco J.
author
Franch i Masferrer, Àngels
author
Castell, Margarida
author
2016-01
Previous studies have demonstrated that cocoa intake decreased Th2 immune-related antibodies in rats. In consequence, we aimed to study in depth this cocoa action, particularly assessing its effect on a rat model of food allergy (FA) and also on an anaphylactic response. The involvement of the intestinal immune system was analyzed to allow the action mechanisms to be investigated. The role of cocoa flavonoids in the anti-allergic properties of cocoa was also established. Brown Norway rats were fed either a reference diet or diets containing conventional cocoa (CC) or non-fermented cocoa (NFC). FA to ovalbumin (OVA) was induced and, later, an anaphylactic response was provoked. As expected, the synthesis of anti-OVA IgE and other Th2-related antibodies was inhibited by CC diet. In addition, the release of mast cell protease II after anaphylaxis was partially prevented by CC, although other variables were not modified. The CC diet also attenuated the increase of some Th2-related cytokines released from mesenteric lymph node and spleen cells, and modulated the intestinal gene expression of molecules involved in allergic response. These results demonstrated the local and systemic influence of CC diet. The effects of the NFC diet were weaker than those of CC, suggesting that cocoa components other than flavonoids play a role in cocoa's action. In conclusion, by acting on intestinal and systemic immune functions, a cocoa-enriched diet in rats exhibited a protective effect against FA and partially against anaphylaxis, making this a food of high interest to the fields of health and immunonutrition.
0955-2863
http://hdl.handle.net/2445/166758
654610
Effect of a cocoa-enriched diet on immune response and anaphylaxis in a food allergy model in Brown Norway rats
oai:diposit.ub.edu:2445/1668172024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Quintanilla-Casas, Beatriz
author
Dulsat-Serra, Neus
author
Cortés Francisco, Nuria
author
Caixach Gamisans, Josep
author
Vichi, S. (Stefania)
author
2015-07-10
In the present paper, we present a simple, reliable, selective and sensitive method for the identification and quantification of volatile thiols at trace levels in coffee brews. A simultaneous derivatization/ extraction procedure followed by liquid chromatography electrospray high-resolution mass spectrometry is proposed and adapted to coffee brew matrix, and the performance of the method is evaluated. The linearity, sensitivity, recovery and both the intra-day and inter-day accuracy were all satisfactory. According to established identification criteria, seven target and nine non-target thiols were identified and quantified in coffee brew samples. Several of them are reported here for the first time in coffee brews, and our results are in agreement with previously reported results for coffee powder analyzed using similar analytical approach.
0023-6438
http://hdl.handle.net/2445/166817
653812
Thiols in brewed coffee: assessment by fast derivatization and liquid chromatography high resolution mass spectrometry
oai:diposit.ub.edu:2445/1668582024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Vichi, S. (Stefania)
author
Boynuegri, Pinar
author
Caixach Gamisans, Josep
author
Romero, Agustí
author
2015
To identify critical points during olive mill pre-processing operations, the effect of the closed circuit washing stage on the olives microbiological contamination and the influence of the successive short-term storage on olives and VOO quality were evaluated. Microbiological, physical and chemical parameters were assessed in olives and oils at three mill pre-processing stages: reception, washing and short-term storage. Olive washing in closed loop systems was shown to be a critical control point at the olive mill due to microbiological cross-contamination and fruit physical damage. Moreover, when the olives were short-term stored before oil extraction positive VOO sensory attributes decreased by as much as one point of intensity, as justified by the changes observed in phenolic and lipoxygenase derived compounds. These results confirm the high risk of fruit cross-contamination due to the poor hygiene of the water used in olive mills to wash olive, and point o ut t he e ffect of on VOO quality of a common practice such as short term silo storage of olives.
1438-7697
http://hdl.handle.net/2445/166858
653811
Quality losses in virgin olive oil due to washing and short-term storage before olive milling
oai:diposit.ub.edu:2445/1669372024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7198col_2445_108487col_2445_7199
00925njm 22002777a 4500
dc
Périz, Marta
author
Pérez-Cano, Francisco J.
author
Rodríguez Lagunas, María José
author
Cambras Riu, Trinitat
author
Pastor Soplín, Santiago
author
Best, Iván
author
Castell, Margarida
author
Massot Cladera, Malen
author
2020-05-28
Allergic asthma is one of the most common chronic diseases of the airways, however it still remains underdiagnosed and hence undertreated. Therefore, an allergic asthma rat model would be useful to be applied in future therapeutic strategy studies. The aim of the present study was to develop an objective model of allergic asthma in atopic rats that allows the induction and quantification of anaphylactic shock with quantitative variables. Female Brown Norway rats were intraperitoneally sensitized with ovalbumin (OVA), alum and Bordetella pertussis toxin and boosted a week later with OVA in alum. At day 28, all rats received an intranasal challenge with OVA. Anaphylactic response was accurately assessed by changes in motor activity and body temperature. Leukotriene concentration was determined in the bronchoalveolar lavage fluid (BALF), and total and IgE anti-OVA antibodies were quantified in blood and BALF samples. The asthmatic animals' motility and body temperature were reduced after the shock for at least 20 h. The asthmatic animals developed anti-OVA IgE antibodies both in BALF and in serum. These results show an effective and relatively rapid model of allergic asthma in female Brown Norway rats that allows the quantification of the anaphylactic response.
1661-6596
http://hdl.handle.net/2445/166937
701432
32481675
Development and characterization of an allergic asthma rat model for interventional studies
oai:diposit.ub.edu:2445/1669892024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Garcia Aloy, Mar
author
Ulaszewska, Marynka M.
author
Franceschi, Pietro
author
Estruel Amades, Seila
author
Weinert, Christoph H.
author
Tor-Roca, Alba
author
Urpí Sardà, Mireia
author
Mattivi, Fulvio
author
Andrés Lacueva, Ma. Cristina
author
2020-05-18
Scope: To identify reliable biomarkers of food intake (BFIs) of pulses. Methods and results: A randomized crossover postprandial intervention study is conducted on 11 volunteers who consumed lentils, chickpeas, and white beans. Urine and serum samples are collected at distinct postprandial time points up to 48 h, and analyzed by LC-HR-MS untargeted metabolomics. Hypaphorine, trigonelline, several small peptides, and polyphenol-derived metabolites prove to be the most discriminating urinary metabolites. Two arginine-related compounds, dopamine sulfate and epicatechin metabolites, with their microbial derivatives, are identified only after intake of lentils, whereas protocatechuic acid is identified only after consumption of chickpeas. Urinary hydroxyjasmonic and hydroxydihydrojasmonic acids, as well as serum pipecolic acid and methylcysteine, are found after white bean consumption. Most of the metabolites identified in the postprandial study are replicated as discriminants in 24 h urine samples, demonstrating that in this case the use of a single, noninvasive sample is suitable for revealing the consumption of pulses. Conclusions: The results of the present untargeted metabolomics work reveals a broad list of metabolites that are candidates for use as biomarkers of pulse intake. Further studies are needed to validate these BFIs and to find the best combinations of them to boost their specificity.
1613-4125
http://hdl.handle.net/2445/166989
701095
Discovery of intake biomarkers of lentils, chickpeas and white beans by untargeted LC-MS metabolomics in serum and urine.
oai:diposit.ub.edu:2445/1674582024-03-12T17:02:13Zcom_2445_67490com_2445_7181com_2445_7209com_2445_96580col_2445_67491col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Rabassa Bonet, Montserrat
author
Zamora-Ros, Raul
author
Palau Rodríguez, Magalí
author
Tulipani, Sara
author
Miñarro Alonso, Antonio
author
Bandinelli, Stefania
author
Ferrucci, Luigi
author
Cherubini, Antonio
author
Andrés Lacueva, Ma. Cristina
author
2020-01-07
Scope: The association between self-reported dietary intake and urinary metabolomic markers of habitual nut exposure with cognitive decline over a 3-year follow-up in an older Italian population is prospectively evaluated. Methods and results: A total of 119 older participants are selected, based on self-referred nut intake: the non-nut consumer (n = 72) and the regular consumer (≥2.9 g d-1 , n = 47). Nut exposure is measured at baseline either with the use of a validated food frequency questionnaire or with an HPLC-Q-ToF-MS metabolomic approach. Three years after, 28 from the nonconsumers and 10 from the consumers experienced cognitive decline. Dietary nut exposure is characterized by urinary metabolites of polyphenols and fatty acids pathways. Nut consumption estimated either by the dietary marker or by the urinary marker model is in both cases associated with less cognitive decline (OR: 0.78, 95% CI: 0.61,0.99; p = 0.043 and OR: 0.995, 95% CI: 0.991,0.999; p = 0.016, respectively) with AUCs 73.2 (95% CI: 62.9, 83.6) and 73.1 (62.5, 83.7), respectively. Conclusions: A high intake of nuts may protect older adults from cognitive decline. Metabolomics provides accurate and complementary information of the nut exposure and reinforces the results obtained using dietary information.
1613-4125
http://hdl.handle.net/2445/167458
694887
31755209
Habitual Nut Exposure, Assessed by Dietary and Multiple Urinary Metabolomic Markers, and Cognitive Decline in Older Adults: The InCHIANTI Study.
oai:diposit.ub.edu:2445/1674602024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Marco Ramell, Anna
author
Tulipani, Sara
author
Palau Rodríguez, Magalí
author
González-Domínguez, Raúl
author
Miñarro Alonso, Antonio
author
Jáuregui Pallarés, Olga
author
Sànchez, Àlex (Sànchez Pla)
author
Macias-Gonzalez, Manuel
author
Cardona, Fernando
author
Tinahones, Francisco J.
author
Andrés Lacueva, Ma. Cristina
author
2018-06-15
This study explores the metabolic profiles of concordant/discordant phenotypes of high insulin resistance (IR) and obesity. Through untargeted metabolomics (LC-ESI-QTOF-MS), we analyzed the fasting serum of subjects with high IR and/or obesity ( n = 64). An partial least-squares discriminant analysis with orthogonal signal correction followed by univariate statistics and enrichment analysis allowed exploration of these metabolic profiles. A multivariate regression method (LASSO) was used for variable selection and a predictive biomarker model to identify subjects with high IR regardless of obesity was built. Adrenic acid and a dyglyceride (DG) were shared by high IR and obesity. Uric and margaric acids, 14 DGs, ketocholesterol, and hydroxycorticosterone were unique to high IR, while arachidonic, hydroxyeicosatetraenoic (HETE), palmitoleic, triHETE, and glycocholic acids, HETE lactone, leukotriene B4, and two glutamyl-peptides to obesity. DGs and adrenic acid differed in concordant/discordant phenotypes, thereby revealing protective mechanisms against high IR also in obesity. A biomarker model formed by DGs, uric and adrenic acids presented a high predictive power to identify subjects with high IR [AUC 80.1% (68.9-91.4)]. These findings could become relevant for diabetes risk detection and unveil new potential targets in therapeutic treatments of IR, diabetes, and obesity. An independent validated cohort is needed to confirm these results.
1535-3893
http://hdl.handle.net/2445/167460
683373
Untargeted profiling of concordant/discordant phenotypes of high insulin resistance and obesity to predict the risk of developing diabetes
oai:diposit.ub.edu:2445/1674982024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Alemán, Mercedes
author
Bou Novensà, Ricard
author
Polo, Javier
author
Rodríguez, Carmen
author
Tres Oliver, Alba
author
Codony Salcedo, Rafael
author
Guardiola Ibarz, Francesc
author
2016
Fortification of food products with non-heme or heme iron is a common strategy to overcome nutritional iron deficiency. Heme iron is highly bioavailable but it promotes oxidation, as do other iron forms. Palm oil is widely used in the formulation of bakery products and chocolate fillings. The work reported here aims to delay the onset of oxidation of a palm oil matrix fortified with heme iron, as a model for bakery products, through the use of ascorbyl palmitate (0 and 400 mg/kg) and the co-spray-drying of the heme iron with calcium caseinate in two ratios (heme iron concentrate:caseinate, 2:1 and 1:1, w/w). Primary (peroxide value and lipid hydroperoxide content) and secondary (p-anisidine value and hexanal content) oxidation were measured over one year of storage at room temperature in the dark. The combination of ascorbyl palmitate at 400 mg/kg and the co-spray-dried heme iron in a 1:1 ratio was the treatment that best protected iron fortified samples from oxidation during the storage time.
1438-7697
http://hdl.handle.net/2445/167498
650311
Co-spray-drying of a heme iron ingredient to decrease its pro-oxidant effect in lipid-containing foods
oai:diposit.ub.edu:2445/1678882024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Dulsat-Serra, Neus
author
Quintanilla-Casas, Beatriz
author
Vichi, S. (Stefania)
author
2016-02-13
Thiols are among the compounds that have the greatest impact on the flavor of coffee. Due to their extremely low odor thresholds, they have a significant sensory impact even at very low concentrations. Thiols are formed during coffee roasting and are described as the key odorants responsible for the typical 'coffee' and 'roasty' odor notes, greatly influencing the sensory characteristics of coffee. They are particularly reactive and prone to oxidation; their rapid depletion after preparation of a coffee brew and during storage of roasted coffee has been associated with sensory quality decrease and coffee going stale. For these reasons, their determination and insight into their formation and degradation mechanisms could help us to preserve the sensory quality of coffee and to modulate its sensory features. Coffee aroma has been widely studied in recent decades, and it has become evident that the role of certain volatile thiols is paramount. Nevertheless, a limited number of studies have specifically addressed this class of compounds, and several aspects have not yet been satisfactorily elucidated. The aim of this review is to provide an overview of the current state on knowledge about coffee thiols, focusing on their occurrence, determination, sensory impact, formation and evolution in roasted and brewed coffee.
0963-9969
http://hdl.handle.net/2445/167888
661541
Volatile thiols in coffee: A review on their formation, degradation, assessment and influence on coffee sensory quality
oai:diposit.ub.edu:2445/1679302024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Trullàs, C
author
Fontanillas, R.
author
Tres Oliver, Alba
author
Sala, Roser
author
2017
Re-esterified vegetable oils are obtained from a chemical esterification reaction between vegetable acid oils and glycerol. Due to their properties, it is expected that they have a higher nutritive value than their corresponding acid oils and a better digestibility than their native counterparts. The aim of the present study was to determine the effect of reesterified oils with a different monoacylglycerol (MAG) and diacylglycerol (DAG) content, produced from palm or rapeseed, on fatty acid digestibility in gilthead sea bream (Sparus aurata). Triplicate groups of fish were fed nine experimental diets containing different oils during 28 days. For each source, four different types of oil were used: native, re-esterified low or high in MAG and DAG and acid. A commercial fish oil was used for the control diet. Diets containing re-esterified oils had better apparent digestibility coefficients (ADC) of total fatty acids than acid oil diets. Reesterified oils do not negatively affect apparent digestibility coefficients of fatty acids when compared to their corresponding native oils and could be incorporated as a source of energy in diets for gilthead sea bream. An improvement in digestibility compared to the native oil diet was only obtained in palm re-esterified oil high in MAG and DAG.
1353-5773
http://hdl.handle.net/2445/167930
657037
Fatty acid digestibility in gilthead sea bream fed diets containing native, re-esterified or acid vegetable oils.
oai:diposit.ub.edu:2445/1688102024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Casas Agustench, Patricia
author
Cherubini, Antonio
author
Andrés-Lacueva, C.
author
2016-10-07
PURPOSE OF REVIEW: Healthy aging is a public health priority. The maintenance of adequate physical function is recognized as a key element of healthy aging. In recent years, scientific evidence has increased concerning the ability of lipids, in particular omega 3 polyunsaturated fatty acids (n-3 PUFAs), to positively influence muscle and overall physical function in older patients. The article will critically review observational as well as intervention studies on this topic, and it will elucidate the potential biological mechanisms underlying the beneficial effects of n-3 PUFA on physical function. RECENT FINDINGS: Observational studies and clinical trials performed in healthy older patients and in older patients with chronic diseases mostly found positive effects of n-3 PUFA on muscle metabolism, muscle strength and in general physical function. SUMMARY: Although the use of n-3 PUFA might represent an important intervention to preserve physical function in older adults, several key questions still need to be answered. Above all, large randomized controlled trials should be performed to confirm the utility of n-3 PUFA as therapeutic agents to prevent and treat physical function decline in old age.
1363-1950
http://hdl.handle.net/2445/168810
664840
27753664
Lipids and physical function in older adults
oai:diposit.ub.edu:2445/1721842024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_20400col_2445_108487col_2445_20401
00925njm 22002777a 4500
dc
Galindo-Luján, Rocío
author
Pont Villanueva, Laura
author
Sanz Nebot, María Victoria
author
Benavente Moreno, Fernando J. (Julián)
author
2020-09-28
Quinoa (Chenopodium quinoa Willd.) is an andean grain with exceptional nutritional properties that has been progressively introduced in western countries as a protein-rich super food with a broad amino acid spectrum. Quinoa is consumed as whole grain, but it is also milled to produce high-value flour, which is susceptible to adulteration. Therefore, there is a growing interest in developing novel analytical methods to get further information about quinoa at the chemical level. In this study, we developed a rapid and simple capillary electrophoresis-ultraviolet absorption diode array detection (CE-UV-DAD) method to obtain characteristic multiwavelength electrophoretic profiles of soluble protein extracts from different quinoa grain varieties. Then, advanced chemometric methods (i.e. multivariate curve resolution alternating least squares, MCR-ALS, followed by principal component analysis, PCA, and partial least squares discriminant analysis, PLS-DA) were applied to deconvolute the components present in the electropherograms and classify the quinoa varieties according to their differential protein composition.
0308-8146
http://hdl.handle.net/2445/172184
704535
Classification of quinoa varieties based on protein fingerprinting by capillary electrophoresis with ultraviolet absorption diode array detection and advanced chemometrics
oai:diposit.ub.edu:2445/1731052024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7209col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
Tres Oliver, Alba
author
Tarnovska, Natalia
author
Varona, Elisa
author
Quintanilla-Casas, Beatriz
author
Vichi, S. (Stefania)
author
Gibert, Anna
author
Vilchez, Elisenda
author
Guardiola Ibarz, Francesc
author
2020-06-02
The objective is to verify if gluten-free (GF) and gluten-containing (G) breads differ in their sodium content and lipid profile. Samples of GF (n=20) and G (n=14) sliced white sandwich bread of commercial brands most frequently consumed in Spain were collected. The fatty acid (FA) composition and the contents of sodium, fat, cholesterol and phytosterols were determined. Sodium, fat and cholesterol contents were significantly higher in GF bread. The FA composition also differed, while G breads declared in most instances the use of sunflower oil as fat ingredient and presented a higher polyunsaturated FA percentage; GF breads declared a wide variety of fats and oils as ingredients (coconut, palm, olive, sunflower, etc.) which was reflected in their FA profile. Cholesterol content was higher in GF bread because five samples declared the use of whole egg, while G samples did not include any egg product in their formulas. Phytosterol content was higher in G bread but its variability was greater in GF bread. In conclusion, nutritional quality of GF bread varied depending on the ingredients used and might be lower than that of G bread. However, these differences in composition could be reduced or eliminated through changes in the formulation of GF bread. Moreover, the comparison of the results obtained in our laboratory for fat and salt content with the declared contents on the labels showed a much higher deviation for GF samples and it can be concluded that the quality of the nutritional information declared was lower in GF samples.
0921-9668
http://hdl.handle.net/2445/173105
701541
32488604
Determination and Comparison of the Lipid Profile and Sodium Content of Gluten-Free and Gluten-Containing Breads from the Spanish Market
oai:diposit.ub.edu:2445/1740302024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_96580col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Simonin, Scott
author
Roullier-Gall, Chloé
author
Ballester, Jordi
author
Schmitt-Kopplin, Philippe
author
Quintanilla-Casas, Beatriz
author
Vichi, S. (Stefania)
author
Peyron, Dominique
author
Alexandre, Hervé
author
Tourdot Maréchale, Raphaëlle
author
2020-05-22
In wine, one method of limiting the addition of sulphites, a harmful and allergenic agent, is bio-protection. This practice consists of the early addition of microorganisms on grape must before fermentation. Non-Saccharomyces yeasts have been proposed as an interesting alternative to sulphite addition. However, scientific data proving the effectiveness of bio-protection remains sparse. This study provides the first analysis of the chemical and microbiological effects of a Metschnikowia pulcherrima strain inoculated at the beginning of the red winemaking process in three wineries as an alternative to sulphiting. Like sulphiting, bio-protection effectively limited the growth of spoilage microbiota and had no influence on the phenolic compounds protecting musts and wine from oxidation. The bio-protection had no effect on the volatile compounds and the sensory differences were dependent on the experimental sites. However, a non-targeted metabolomic analysis by FTICR-MS highlighted a bio-protection signature.
1664-302X
http://hdl.handle.net/2445/174030
701757
32612594
Bio-Protection as an Alternative to Sulphites: Impact on Chemical and Microbial Characteristics of Red Wines
oai:diposit.ub.edu:2445/1740732024-03-12T17:02:13Zcom_2445_7201com_2445_7181com_2445_7203com_2445_96580col_2445_7202col_2445_7204col_2445_108487
00925njm 22002777a 4500
dc
Anfruns-Estrada, Eduard
author
Sabrià Sunyé, Aurora
author
Fuentes Pardo, Cristina
author
Sabaté, Sara
author
Razquin, Efrén
author
Cornejo Sánchez, Thais
author
Bartolomé, Rosa
author
Torner Gràcia, Núria
author
Izquierdo, Conchita
author
Soldevila, Núria
author
Coronas, Lorena
author
Domínguez García, Àngela
author
Pintó Solé, Rosa María
author
Bosch, Albert
author
Guix Arnau, Susana
author
2020-11-30
Norovirus infections are a leading cause of acute gastroenteritis outbreaks worldwide and across all age groups, with two main genogroups (GI and GII) infecting humans. The aim of our study was to investigate the occurrence of norovirus in saliva samples from individuals involved in outbreaks of acute gastroenteritis in closed and semiclosed institutions, and its relationship with the virus strain, virus shedding in stool, the occurrence of symptoms, age, and the secretor status of the individual. Epidemiological and clinical information was gathered from norovirus outbreaks occurring in Catalonia, Spain during 2017-2018, and stool and saliva samples were collected from affected and exposed resident individuals and workers. A total of 347 saliva specimens from 25 outbreaks were analyzed. Further, 84% of individuals also provided a paired stool sample. For GII infections, norovirus was detected in 17.9% of saliva samples from symptomatic cases and 5.2% of asymptomatic individuals. Positivity in saliva occurred in both secretors and nonsecretors. None of the individuals infected by norovirus GI was positive for the virus in saliva. Saliva positivity did not correlate with any of the studied symptoms but did correlate with age 65 years old. Individuals who were positive in saliva showed higher levels of virus shedding in stool. Mean viral load in positive saliva was 3.16 1.08 log10 genome copies/mL, and the predominance of encapsidated genomes was confirmed by propidium monoazide (PMA)xx-viability RTqPCR assay. The detection of norovirus in saliva raises the possibility of oral-to-oral norovirus transmission during the symptomatic phase and, although to a lesser extent, even in cases of asymptomatic infections.
1999-4915
http://hdl.handle.net/2445/174073
705182
33266188
Detection of Norovirus in saliva samples from acute gastroenteritis cases and asymptomatic subjects: association with age and higher shedding in stool
oai:diposit.ub.edu:2445/1762942024-03-12T17:02:13Zcom_2445_7201com_2445_7181com_2445_96580col_2445_7202col_2445_108487
00925njm 22002777a 4500
dc
Vu, Diem Lan
author
Sabrià Sunyé, Aurora
author
Aregall, Núria
author
Michl, Kristina
author
Rodríguez Garrido, Virginia
author
Goterris, Lidia
author
Bosch, Albert
author
Pintó Solé, Rosa María
author
Guix Arnau, Susana
author
2019-01-27
A remarkable percentage of acute gastroenteritis cases remain etiologically undiagnosed. The aim of the study was to determine the prevalence of common and emerging enteric viruses, such as novel human astroviruses, among undiagnosed samples from children with acute gastroenteritis. Epidemiological studies for novel human astroviruses are still scarce. Stool samples collected over two consecutive winter seasons (2016-2017) from children with gastroenteritis in Spain, which were negative for bacteria, rotavirus, and adenovirus by routine diagnostics were screened by real-time RT-PCR assays for the presence of classical and novel astrovirus, rotavirus, norovirus GI and GII, sapovirus, and adenovirus. Overall, 220/384 stool samples (57.3%) were positive for at least one virus. Co-infections were identified in 21% of cases. Among a total of 315 viruses identified, adenovirus was the most prevalent (n = 103), followed by rotavirus (n = 51), sapovirus (n = 50), classical astrovirus (n = 43), novel astroviruses (n = 42), and norovirus (n = 26). Novel astroviruses were present in 13.3% of virus-positive cases. Most novel astroviruses were found in children <2-year-old (30/39 children, 77%, p = 0.01) and were found in co-infection (66%). Only classical astroviruses demonstrated significant differences in the Cq values during mono-infections compared to co-infections. In conclusion, common enteric viruses may be frequently found in children with undiagnosed gastroenteritis, indicating the need to implement more sensitive diagnostic methods. Novel astroviruses circulate in the community and could be the cause of gastroenteritis among young children.
1999-4915
http://hdl.handle.net/2445/176294
695111
31252663
Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in Spain
oai:diposit.ub.edu:2445/1763182024-03-12T17:02:13Zcom_2445_21591com_2445_7181com_2445_59248com_2445_96580com_2445_7209col_2445_25582col_2445_59249col_2445_108487col_2445_7210
00925njm 22002777a 4500
dc
López-Yerena, Anallely
author
Vallverdú i Queralt, Anna
author
Mols, Raf
author
Augustijns, Patrick
author
Lamuela Raventós, Rosa Ma.
author
Escribano Ferrer, Elvira
author
2020-12-17
Recently, in February 2020, we published a study exploring the intestinal absorption and metabolism of oleocanthal (OLC) in rats. A single-pass intestinal perfusion technique (SPIP) was used, involving simultaneous sampling from the luminal perfusate and mesenteric blood. Later, comments on our published paper were released, requesting clarification of specific data. In this detailed reply, we hope to have addressed and clarified all the concerns of A. Kaddoumi and K. El Sayed and that the scientific community will benefit from both the study and the comments it has generated.
1999-4923
http://hdl.handle.net/2445/176318
707308
Reply to 'Comment on López-Yerena et al. 'Absorption and intestinal metabolic profile of oleocanthal in rats' Pharmaceutics 2020, 12, 134'
oai:diposit.ub.edu:2445/1763002024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_7198col_2445_108487col_2445_7199
00925njm 22002777a 4500
dc
González Solé, Francesc
author
Criado Mesas, Lourdes
author
Villodre, Carmen
author
García, Wellington C.
author
Farré, Mercè
author
Borda, Elisabet
author
Pérez-Cano, Francisco J.
author
Folch, Josep M.
author
Solà Oriol, David
author
Pérez, José F.
author
2020-12-10
This study was conducted to investigate the effects of dietary supplementation of porcine digestible peptides (PDP), spray-dried plasma (SDP), or a combination of both, on growth performance and the expression of genes related to intestinal function of weaned pigs. A total of 180 piglets (trial 1) and 198 piglets (trial 2) were used to evaluate the partial substitution of soybean ingredients with 2% SDP or 2% PDP (trial 1), and with 3% SDP or the combination of 1% SDP and 2% PDP (SDP-PDP; trial 2) during the pre-starter period (0-14 days). The gene expression of 56 genes was quantified in a qPCR platform in jejunum and ileum samples obtained from piglets 14 d after weaning (trial 2). Piglets fed SDP, PDP and SDP-PDP had a higher body weight (BW), average daily gain (ADG) and feed efficiency (G:F) than the soybean control on day 14 (p < 0.05). In addition, the combination of SDP and PDP upregulated ten genes in jejunum samples (p < 0.05) related to intestinal function. More research is needed to confirm that gene expression upregulation by PDP in combination with SDP has an impact on intestinal function and to elucidate its underlying mechanisms.
2076-2615
http://hdl.handle.net/2445/176300
708738
33321976
Porcine Digestible Peptides (PDP) in Weanling Diets Regulates the Expression of Genes Involved in Gut Barrier Function, Immune Response and Nutrient Transport in Nursery Pigs
oai:diposit.ub.edu:2445/1763382024-03-12T17:02:13Zcom_2445_107205com_2445_7181com_2445_7209com_2445_96580com_2445_67490col_2445_107206col_2445_7210col_2445_108487col_2445_67491
00925njm 22002777a 4500
dc
Marhuenda-Muñoz, María
author
Rinaldi de Alvarenga, José Fernando
author
Hernáez, Álvaro
author
Tresserra i Rimbau, Anna
author
Martínez-González, Miguel Ángel, 1957-
author
Salas Salvadó, Jordi
author
Corella Piquer, Dolores
author
Malcampo, Mireia
author
Martínez, José Alfredo
author
Alonso Gómez, Ángel M.
author
Wärnberg, Julia
author
Vioque, Jesús
author
Romaguera, Dora
author
López Miranda, José
author
Estruch Riba, Ramon
author
Tinahones, Francisco J.
author
Lapetra, José
author
Serra Majem, Lluís
author
Bueno Cavanillas, Aurora
author
Tur, Josep A.
author
Sánchez, Vicente Martín
author
Pintó Sala, Xavier
author
Delgado Rodríguez, Miguel
author
Matía Martín, Pilar
author
Vidal i Cortada, Josep
author
Vázquez, Clotilde
author
Daimiel, Lidia
author
Ros Rahola, Emilio
author
Serra-Mir, Mercè
author
Vázquez Ruiz, Zenaida
author
Nishi, Stephanie K.
author
Sorlí, Jose V.
author
Zomeño, María Dolores
author
Zulet, M. Angeles
author
Vaquero Luna, Jessica
author
Carabaño Moral, Rosa
author
Notario Barandiaran, Leyre
author
Morey, Marga
author
García Ríos, Antonio
author
Gómez Pérez, Ana M.
author
Santos Lozano, José Manuel
author
Buil Cosiales, Pilar
author
Basora, Josep
author
Portolés, Olga
author
Schröder, Helmut
author
Abete, Itziar
author
Salaverria Lete, Itziar
author
Toledo, Estefanía
author
Babio, Nancy
author
Fitó, Montse
author
Martínez Huélamo, Miriam
author
Lamuela Raventós, Rosa Ma.
author
2021-03-17
Carotenoids are pigments contained mainly in fruit and vegetables (F&V) that have beneficial effects on cardiometabolic health. Due to their lipophilic nature, co-ingestion of fat appears to increase their bioavailability via facilitating transfer to the aqueous micellar phase during digestion. However, the extent to which high fat intake may contribute to increased carotenoid plasma concentrations is still unclear. The objective was to examine the degree to which the consumption of different amounts of both carotenoid-rich foods and fats is associated with plasma carotenoid concentrations within a Mediterranean lifestyle context (subsample from the PREDIMED-Plus study baseline) where consumption of F&V and fat is high. The study population was categorized into four groups according to their self-reported consumption of F&V and fat. Carotenoids were extracted from plasma samples and analyzed by HPLC-UV-VIS-QqQ-MS/MS. Carotenoid systemic concentrations were greater in high consumers of F&V than in low consumers of these foods (+3.04 μmol/L (95% CI: 0.90, 5.17), p-value = 0.005), but circulating concentrations seemed to decrease when total fat intake was very high (-2.69 μmol/L (-5.54; 0.16), p-value = 0.064). High consumption of F&V is associated with greater systemic levels of total carotenoids, in particular when fat intake is low-to-moderate rather than very high.
http://hdl.handle.net/2445/176338
711670
33802859
High Fruit and Vegetable Consumption and Moderate Fat Intake Are Associated with Higher Carotenoid Concentration in Human Plasma
oai:diposit.ub.edu:2445/1793772024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Zúñiga López, María Carolina
author
Maturana, Gabriela
author
Campmajó Galván, Guillem
author
Saurina, Javier
author
Núñez Burcio, Oscar
author
2021-07-19
Consumers' interest in foods that are nutritionally balanced and with health benefits has increased. The food industry is paying attention to the use of the ancestral seed Salvia hispanica L., commonly known as chia. At present, only the chia seeds, which are a natural source of omega-3 and omega-6, fiber, proteins, and natural antioxidants, are commercialized. Although some studies reveal the presence of several bioactive compounds like polyphenols (e.g., vitexin, orientin, and some hydroxycinnamic acids) in chia leaf methanolic extracts, the chia plant is commonly used as fertilizer or treated as waste after harvest. Therefore, it can represent a by-product that could be considered a great source of bioactive compounds with unexplored potential in medicine and food industry applications. In this work, UHPLC-HRMS (Q-Orbitrap) was employed to tentatively identify and determine bioactive compounds present in different leaf extracts of chia plants of black and white seed phenotype obtained with solvents of different polarity (ethanol, ethyl acetate, dichloromethane, and hexane) to address chia plant by-product revalorization. The chemical antioxidant capacity was also studied and correlated to the found bioactive compounds. In these experiments, black chia showed a higher antioxidant capacity than white chia in the ethanolic extracts. Moreover, experiments on cellular antioxidant activity were also performed with a predominance of the white chia extract. It is noted that the cellular antioxidant activity results make chia ethanolic extracts promising antioxidants.
2076-3921
http://hdl.handle.net/2445/179377
713378
34356383
Determination of bioactive compounds in sequential extracts of chia leaf (Salvia hispanica L.) using UHPLC-HRMS (Q-Orbitrap) and a global evaluation of antioxidant in vitro capacity
oai:diposit.ub.edu:2445/1798472024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_107205com_2445_7203com_2445_67490com_2445_7209col_2445_108487col_2445_107206col_2445_7204col_2445_67491col_2445_7210col_2445_15202
00925njm 22002777a 4500
dc
Domínguez López, Inés
author
Marhuenda-Muñoz, María
author
Tresserra i Rimbau, Anna
author
Hernáez, Álvaro
author
Moreno Aznárez, Juan José
author
Martínez-González, Miguel Ángel, 1957-
author
Salas Salvadó, Jordi
author
Corella Piquer, Dolores
author
Fitó Colomer, Montserrat
author
Martínez, J. Alfredo, 1957-
author
Alonso Gómez, Ángel M.
author
Wärnberg, Julia
author
Vioque, Jesús
author
Romaguera, Dora
author
López Miranda, José
author
Bernal López, M. Rosa
author
Lapetra, José
author
Serra Majem, Lluís
author
Bueno Cavanillas, Aurora
author
Tur, Josep Antoni
author
Martín Sánchez, Vicente
author
Pintó Sala, Xavier
author
Delgado Rodríguez, Miguel
author
Matía Martín, Pilar
author
Vidal, Josep
author
Vázquez, Clotilde
author
Daimiel, Lidia
author
Serra Mir, Mercè
author
Vázquez Ruiz, Zenaida
author
Nishi, Stephanie K.
author
Sorli, Jose V.
author
Castañer, Olga
author
Abete, Itziar
author
Vaquero Luna, Jessica
author
Carabaño Moral, Rosa
author
Asencio, Alberto
author
Prohens, Lara
author
García Rios, Antonio
author
Casas Rodríguez, Rosa M.
author
Gómez Pérez, Ana María
author
Santos Lozano, Jose Manuel
author
Razquin, Cristina
author
Martínez, María Ángeles
author
Saiz, Carmen
author
Robledo Pastor, Vanesa
author
Zulet, M. Angeles
author
Salaverria Lete, Itziar
author
Eguaras, Sonia
author
Babio, Nancy
author
Malcampo, Mireia
author
Ros Rahola, Emilio
author
Estruch Riba, Ramon
author
López Sabater, María del Carmen
author
Lamuela Raventós, Rosa Ma.
author
2021-07-28
Scope: Plasma fatty acids (FAs) are associated with the development of cardiovascular diseases and metabolic syndrome. The aim of our study is to assess the relationship between fruit and vegetable (F&V) consumption and plasma FAs and their subtypes. Methods and results: Plasma FAs are assessed in a cross-sectional analysis of a subsample of 240 subjects from the PREDIMED-Plus study. Participants are categorized into four groups of fruit, vegetable, and fat intake according to the food frequency questionnaire. Plasma FA analysis is performed using gas chromatography. Associations between FAs and F&V consumption are adjusted for age, sex, physical activity, body mass index (BMI), total energy intake, and alcohol consumption. Plasma saturated FAs are lower in groups with high F&V consumption (-1.20 mg cL-1 [95% CI: [-2.22, -0.18], p-value = 0.021), especially when fat intake is high (-1.74 mg cL-1 [95% CI: [-3.41, -0.06], p-value = 0.042). Total FAs and n-6 polyunsaturated FAs tend to be lower in high consumers of F&V only in the high-fat intake groups. Conclusions: F&V consumption is associated with lower plasma saturated FAs when fat intake is high. These findings suggest that F&V consumption may have different associations with plasma FAs depending on their subtype and on the extent of fat intake.
1613-4133
http://hdl.handle.net/2445/179847
714642
34273124
Fruit and Vegetable Consumption is Inversely Associated with Plasma Saturated Fatty Acids at Baseline in Predimed Plus Trial
oai:diposit.ub.edu:2445/1799952024-03-12T17:02:13Zcom_2445_7203com_2445_7181com_2445_7982com_2445_7209com_2445_67490com_2445_107205com_2445_96580col_2445_7204col_2445_7983col_2445_7210col_2445_67491col_2445_107206col_2445_108487
00925njm 22002777a 4500
dc
Domínguez López, Inés
author
Parilli Moser, Isabella
author
Arancibia Riveros, Camila
author
Tresserra i Rimbau, Anna
author
Martínez González, Miguel Angel
author
Ortega Azorín, Carolina
author
Salas Salvadó, Jordi
author
Castañer, Olga
author
Lapetra, José
author
Arós, Fernando
author
Fiol, Miquel
author
Serra Majem, Lluís
author
Pintó Sala, Xavier
author
Gómez Gracia, Enrique
author
Ros, Emilio
author
Lamuela Raventós, Rosa Ma.
author
Estruch, Ramon
author
2021-08-22
Postmenopausal women are at higher risk of developing cardiovascular diseases due to changes in lipid profile and body fat, among others. The aim of this study was to evaluate the association of urinary tartaric acid, a biomarker of wine consumption, with anthropometric (weight, waist circumference, body mass index (BMI), and waist-to-height ratio), blood pressure, and biochemical variables (blood glucose and lipid profile) that may be affected during the menopausal transition. This sub-study of the PREDIMED (Prevención con Dieta Mediterránea) trial included a sample of 230 women aged 60-80 years with high cardiovascular risk at baseline. Urine samples were diluted and filtered, and tartaric acid was analyzed by liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Correlations between tartaric acid and the study variables were adjusted for age, education level, smoking status, physical activity, BMI, cholesterol-lowering, antihypertensive, and insulin treatment, total energy intake, and consumption of fruits, vegetables, and raisins. A strong association was observed between wine consumption and urinary tartaric acid (0.01 μg/mg (95% confidence interval (CI): 0.01, 0.01), p-value < 0.001). Total and low-density lipoprotein (LDL) cholesterol were inversely correlated with urinary tartaric acid (-3.13 μg/mg (-5.54, -0.71), p-value = 0.016 and -3.03 μg/mg (-5.62, -0.42), p-value = 0.027, respectively), whereas other biochemical and anthropometric variables were unrelated. The results suggest that wine consumption may have a positive effect on cardiovascular health in postmenopausal women, underpinning its nutraceutical properties.
2072-6643
http://hdl.handle.net/2445/179995
714464
34445043
Urinary Tartaric Acid, a Biomarker of Wine Intake, Correlates with Lower Total and LDL Cholesterol
oai:diposit.ub.edu:2445/1822362024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Galindo-Luján, Rocío
author
Pont Villanueva, Laura
author
Minic, Zoran
author
Berezovski, Maxim V.
author
Sanz Nebot, María Victoria
author
Benavente Moreno, Fernando J. (Julián)
author
2021-11-30
Quinoa seed proteins are of prime importance in human nutrition and in plant breeding for cultivar identification and improvement. In this study, proteins from seeds of black, red, white quinoa from Peru and white quinoa from Bolivia (also known as royal) were extracted, digested and analyzed by nano-liquid chromatography coupled to Orbitrap tandem mass spectrometry (LC-MS/MS). The raw mass spectra data were processed for identification and label-free quantification (LFQ) using MaxQuant/Andromeda against a specific quinoa database from The National Center for Biotechnology Information (NCBI). In total, 1,211 quinoa proteins (85 were uncharacterized) were identified. Inspection and visualization using Venn diagrams, heat maps and Gene Ontology (GO) graphs revealed proteome similarities and differences between the four varieties. The presented data provides the most comprehensive experimental quinoa seed proteome map existing to date in the literature, as a starting point for more specific characterization and nutritional studies of quinoa and quinoa-containing foodstuff.
0308-8146
http://hdl.handle.net/2445/182236
714977
Characterization and differentiation of quinoa seed proteomes by label-free mass spectrometry-based shotgun proteomics
oai:diposit.ub.edu:2445/1824252024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Campmajó Galván, Guillem
author
Saurina, Javier
author
Núñez Burcio, Oscar
author
2022
The rise of food fraud practices, affecting a wide variety of goods and their specific characteristics (e.g., quality or geographical origin), demands rapid high-throughput analytical approaches to ensure consumers protection. In this context, this study assesses flow injection analysis coupled to high-resolution mass spectrometry (FIA−HRMS), using a fingerprinting approach and combined with chemometrics, to address four food authentication issues: (i) the geographical origin of three Spanish red wines, (ii) the geographical origin of three European paprikas, (iii) the distinction of olive oil from other vegetable oils and (iv) the assessment of its quality category. In each case, negative and positive ionisation FIA−HRMS fingerprints, and two different data fusion strategies, were evaluated. After external validation, excellent classification accuracies were reached. Moreover, high-resolution mass spectrometry (HRMS) allowed sample matrices characterisation by the putative identification of the most common ions.
0308-8146
http://hdl.handle.net/2445/182425
715833
FIA−HRMS fingerprinting subjected to chemometrics as a valuable tool to address food classification and authentication: application to red wine, paprika, and vegetable oil samples
oai:diposit.ub.edu:2445/1828022024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_9244col_2445_108487col_2445_9245col_2445_15202
00925njm 22002777a 4500
dc
Mille, N.
author
Faure, Stephane
author
Estrader i Bofarull, Marta
author
Yi, Deliang
author
Marbaix, Julien
author
De Masi, D.
author
Soulantica, Katerina
author
Millán, A.
author
Chaudret, Bruno
author
Carrey, J.
author
2021-05-19
Magnetic heating, namely, the use of heat released by magnetic nanoparticles (MNPs) excited with a high-frequency magnetic field, has so far been mainly used for biological applications. More recently, it has been shown that this heat can be used to catalyze chemical reactions, some of them occurring at temperatures up to 700 °C. The full exploitation of MNP heating properties requires the knowledge of the temperature dependence of their heating power up to high temperatures. Here, a setup to perform such measurements is described based on the use of a pyrometer for high-temperature measurements and on a protocol based on the acquisition of cooling curves, which allows us to take into account calorimeter losses. We demonstrate that the setup permits to perform measurements under a controlled atmosphere on solid state samples up to 550 °C. It should in principle be able to perform measurements up to 900 °C. The method, uncertainties, and possible artifacts are described and analyzed in detail. The influence on losses of putting under vacuum different parts of the calorimeter is measured. To illustrate the setup possibilities, the temperature dependence of heating power is measured on four samples displaying very different behaviors. Their heating power increases or decreases with temperature, displaying temperature sensibilities ranging from -2.5 to +4.4% K-1. This setup is useful to characterize the MNPs for magnetically heated catalysis applications and to produce data that will be used to test models permitting to predict the temperature dependence of MNP heating power.
0034-6748
http://hdl.handle.net/2445/182802
716074
A setup to measure the temperature-dependent heating power of magnetically heated nanoparticles up to high temperature.
oai:diposit.ub.edu:2445/1831282024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Pons, Josep
author
Bedmar, Àlex
author
Núñez, Nerea
author
Saurina, Javier
author
Núñez Burcio, Oscar
author
2021-11-26
Tea is a widely consumed drink in the world which is susceptible to undergo adulterations to reduce manufacture costs and rise financial benefits. The development of simple analytical methodologies to assess tea authenticity, and to detect and quantify frauds is an important matter considering the rise of adulteration issues in recent years. In the present study, untargeted HPLC-UV and HPLC-FLD fingerprinting methods were employed to characterize, classify and authenticate tea extracts belonging to different varieties (red, green, black, oolong, and white teas) by partial least squares-discriminant analysis (PLS-DA), as well as to detect and quantify adulteration frauds when chicory was used as the adulterant by partial least squares (PLS) regression, to ensure the authenticity and integrity of foodstuffs. Overall, PLS-DA showed a good classification and grouping of the tea samples according to the tea variety, and except for some white tea extracts, perfectly dis-criminated from the chicory ones. 100% classification rates for the PLS-DA calibration models were achieved except for green and oolong tea when HPLC-FLD fingerprints were employed, which showed classification rates of 96.43% and 95.45%, respectively. Good predictions were also accomplished, showing also, in almost all the cases, a 100% classification rate for prediction, with the exception of white tea and oolong tea when HPLC-UV fingerprints were employed that exhibited a classification rate of 77.78% and 88.89%, respectively. Good PLS results for chicory adulteration detection and quantitation were also accomplished, with calibration, cross-validation, and external validation errors beneath 1.4%, 6.4%, and 3.7%, respectively. Acceptable prediction errors (below 21.7%) were also observed, except for white tea extracts that showed higher errors which were attributed to the low sample variability available.
2304-8158
http://hdl.handle.net/2445/183128
716147
Tea and Chicory Extract Characterization, Classification and Authentication by non-Targeted HPLC-UV-FLD Fingerprinting and Chemometrics
oai:diposit.ub.edu:2445/1837512024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_20400col_2445_108487col_2445_20401
00925njm 22002777a 4500
dc
Vidal-Casanella, Oscar
author
Moreno-Merchan, J.
author
Granados i Juan, Mercè
author
Núñez Burcio, Oscar
author
Saurina, Javier
author
Sentellas, Sonia
author
2022-02-07
The total content of polyphenols and the antioxidant capacity have been estimated in various food and nutraceutical samples including cranberry, raspberry, artichoke, grapevine, green tea, coffee, turmeric, and other medicinal plant extracts. Samples have been analyzed by using two antioxidant assays ¿ferric reducing antioxidant power (FRAP) and Folin-Ciocalteu (FC)¿ and reversed-phase high-performance liquid chromatography (HPLC) focused on providing compositional fingerprints dealing with polyphenolic compounds. A preliminary data exploration by Principal Component Analysis (PCA) has revealed that HPLC fingerprints are suitable chemical descriptors to classify the analyzed samples according to their nature. Besides, chromatographic data have been correlated with antioxidant data using partial least squares (PLS) regression. Regression models have shown a good prediction capacity to estimate the antioxidant activity from chromatographic data, with determination coefficients (R2) of 0.971 and 0.983 for FRAP and FC assays, respectively.
2076-3921
http://hdl.handle.net/2445/183751
718393
Total polyphenol content in food samples and nutraceuticals: antioxidant indices versus high performance liquid chromatography
oai:diposit.ub.edu:2445/1844132024-03-27T13:15:38Zcom_2445_107205com_2445_7181com_2445_7209com_2445_67490com_2445_96580col_2445_107206col_2445_15202col_2445_7210col_2445_67491col_2445_108487
00925njm 22002777a 4500
dc
Tresserra i Rimbau, Anna
author
Castro Barquero, Sara
author
Becerra Tomás, Nerea
author
Babio, Nancy
author
Martínez-González, Miguel Ángel, 1957-
author
Corella Piquer, Dolores
author
Fitó Colomer, Montserrat
author
Romaguera, Dora
author
Vioque, Jesús
author
Alonso Gómez, Ángel M.
author
Wärnberg, Julia
author
Martínez, J. Alfredo, 1957-
author
Serra Majem, Lluís
author
Estruch Riba, Ramon
author
Tinahones, Francisco J.
author
Lapetra, José
author
Pintó Sala, Xavier
author
Tur, Josep Antoni
author
López Miranda, José
author
Cano Ibáñez, Naomi
author
Delgado Rodriguez, Miguel
author
Matía Martín, Pilar
author
Daimiel, Lidia
author
Martín Sánchez, Vicente
author
Vidal i Cortada, Josep
author
Vázquez, Clotilde
author
Ros Rahola, Emilio
author
Basterra, Francisco Javier
author
Fernández de la Puente, María
author
Asensio, Eva M.
author
Castañer, Olga
author
Bullón Vela, Vanessa
author
Tojal Sierra, Lucas
author
Gómez Gracia, Enrique
author
Cases Pérez, Eugenio
author
Konieczna, Jadwiga
author
García-Ríos, Antonio
author
Casañas Quintana , Tamara
author
Bernal López, M. Rosa
author
Santos Lozano, Jose Manuel
author
Esteve Luque, Virginia
author
Bouzas, Cristina
author
Vázquez Ruiz, Zenaida
author
Palau Galindo, Antoni
author
Barragan, Rocio
author
López Grau, Mercè
author
Razquín, Cristina
author
Goicolea Güemez, Leire
author
Toledo, Estefanía
author
Vila Vergaz, Manel
author
Lamuela Raventós, Rosa Ma.
author
Salas Salvadó, Jordi
author
2022-02-04
Previous studies suggested that dietary polyphenols could reduce the incidence and complications of type-2 diabetes (T2D); although the evidence is still limited and inconsistent. This work analyzes whether changing to a diet with a higher polyphenolic content is associated with an improved glucose profile. At baseline, and at 1 year of follow-up visits, 5921 participants (mean age 65.0 ± 4.9, 48.2% women) who had overweight/obesity and metabolic syndrome filled out a validated 143-item semi-quantitative food frequency questionnaire (FFQ), from which polyphenol intakes were calculated. Energy-adjusted total polyphenols and subclasses were categorized in tertiles of changes. Linear mixed-effect models with random intercepts (the recruitment centers) were used to assess associations between changes in polyphenol subclasses intake and 1-year plasma glucose or glycosylated hemoglobin (HbA1c) levels. Increments in total polyphenol intake and some classes were inversely associated with better glucose levels and HbA1c after one year of follow-up. These associations were modified when the analyses were run considering diabetes status separately. To our knowledge, this is the first study to assess the relationship between changes in the intake of all polyphenolic groups and T2D-related parameters in a senior population with T2D or at high-risk of developing T2D
2076-3921
http://hdl.handle.net/2445/184413
719957
9299274
35204199
Adopting a High-Polyphenolic Diet Is Associated with an Improved Glucose Profile: Prospective Analysis within the PREDIMED-Plus Trial
oai:diposit.ub.edu:2445/1848032024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Mancera Arteu, Montserrat
author
Lleshi, Nejsi
author
Sanz Nebot, María Victoria
author
Giménez López, Estela
author
Benavente Moreno, Fernando J. (Julián)
author
2020-01-15
In this study is described an on-line titanium dioxide solid-phase extraction capillary electrophoresis-mass spectrometry (TiO2-SPE-CE-MS) method for the analysis of the glycopeptide glycoforms obtained from the tryptic digests of recombinant human erythropoietin (rhEPO). The O126-glycopeptide of rhEPO was used to optimize the methodology given its importance in quality control of biopharmaceuticals and doping analysis. Several aspects that affect the selective retention and elution, peak efficiency and electrophoretic separation of the O126 glycoforms were investigated to maximize detection sensitivity while minimizing non-specific retention of peptides. Under the optimized conditions, the microcartridge lifetime was around 10 analyses and repeatability was acceptable (%RSD values of 9-11% and 6-11% for migration times and peak areas, respectively). The method was linear between 0.5-50 mg·L-1 and 10-50 mg·L-1 for O126 glycoforms containing NeuAc and NeuGc, respectively, and limits of detection (LODs) were up to 100 times lower than by CE-MS. Although optimized for O-glycopeptides, the method proved also successful for preconcentration of N83-glycopeptides, without compromising the separation between glycopeptide glycoforms with different number of sialic acids. Tryptic digests of other glycoproteins (i.e. human apolipoprotein CIII (APO-C3) and bovine alpha-1-acid glycoprotein (bAGP)) were also analyzed, demonstrating the applicability to glycopeptides with different glycan composition and nature.
0039-9140
http://hdl.handle.net/2445/184803
691980
31892091
Analysis of glycopeptide biomarkers by on-line TiO2 solid-phase extraction capillary electrophoresis-mass spectrometry
oai:diposit.ub.edu:2445/1848192024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Collado, Xavi
author
Campmajó Galván, Guillem
author
Sentellas, Sonia
author
Saurina, Javier
author
Núñez Burcio, Oscar
author
2021
Paprika is a red powder seasoning with a characteristic flavour obtained from the drying and grinding of red pepper fruits of the genus Capsicum (Solanaceae family). In Europe, seven paprika products are distinguished with the protected designation of origin (PDO) label, which ensures a high-quality product through strict requirements, leading to higher retail prices than unlabelled paprika and making them susceptible to fraudulent practices. Contents of polyphenol and phenolic compounds depend on several factors, such as the environmental conditions of the production area. Thus, in the present study, a simple and feasible high-performance liquid chromatography with ultraviolet and fluorescent detection (HPLC-UV-FLD) method was developed to determine 17 polyphenols in paprika samples, aiming to authenticate them through chemometrics. Reversed-phase chromatographic separation was optimised, using a C18 column and 0.1% formic acid aqueous solution and acetonitrile as the mobile phase components. The proposed methodology exhibited limits of detection below 0.9 mg L−1, as well as good linearity (R2 ≥ 0.984), precision (RSD day-to-day values below 24%), and trueness (relative errors below 14%). Moreover, compound confirmation was carried out via high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). The proposed methodology was applied to 109 paprika samples, including samples from Spain (La Vera PDO, Murcia PDO, and Mallorca PDO), Hungary, and the Czech Republic. The obtained HPLC-UV-FLD polyphenolic profiles were employed as sample chemical descriptors to authenticate paprika geographical origin using a classification decision tree constructed via partial least squares regression-discriminant analysis (PLS-DA) models. As a result, a sample classification rate of 87.8% was reached after external validation. Moreover, two different paprika geographical origin blend scenarios (La Vera vs. Murcia and the Czech Republic vs. Murcia) were evaluated through partial least squares (PLS) regression, allowing blend percentage prediction with errors below 10.8% after external validation.
2673-9976
http://hdl.handle.net/2445/184819
722449
Targeted HPLC-UV-FLD Polyphenolics to Assess Paprika Geographical Origin
oai:diposit.ub.edu:2445/1848632024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_20400col_2445_108487col_2445_20401
00925njm 22002777a 4500
dc
Mancera Arteu, Montserrat
author
Giménez López, Estela
author
Sancho, Jaime
author
Sanz Nebot, María Victoria
author
2020-03-12
Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurred in its glycans in collagen-induced arthritis (CIA) samples. SDS-PAGE and nanoLC-MS/MS were used to monitor the IAC purification performance. Afterwards, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice. In addition, multivariate data analysis was applied to identify the most meaningful glycan isomers for the discrimination between control and pathological samples. Partial least squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3 and H5N4S3_2). Moreover, some of these glycan isomers also seemed to be related with the progression of CIA, especially H6N5S2 and H6N5S3_2, as their overexpression increased with the clinical score of the pathology. Hence, the established methodology provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.
1535-3893
http://hdl.handle.net/2445/184863
699942
32162920
Alterations in the glycan profile of mouse transferrin: new insights in collagen-induced arthritis
oai:diposit.ub.edu:2445/1858842024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Granell Geli, Biel
author
Izquierdo-Llopart, Anais
author
Sahuquillo Estrugo, Àngels
author
López Sánchez, José Fermín
author
Saurina, Javier
author
2022-03-01
t: Samples from the different processing stages in the elaboration of sparkling wine (cava) including must, base wine, and sparkling wine of Pinot Noir and Xarel·lo grape varieties from different vineyard qualities (A, B, C, D) have been analyzed by inductively coupled plasma (ICP) techniques to determine their elemental composition. The resulting data has been used to characterize these products according to oenological features and product qualities. For this purpose, box plot diagrams, bar charts, and principal components analysis (PCA) have been used. The study of the behavior of each given species has pointed out the relevance of some elements as markers or descriptors of winemaking processes. Among others, Cu and K are abundant in musts and their concentrations progressively decrease through the cava production process. S levels suddenly increase at the base wine step (and further decay) due to the addition of sulfites as preserving agents. Finally, concentrations of Na, Ca, Fe, and Mg increase from the first fermentation due to the addition of clarifying agents such as bentonite. PCA has been applied to try to extract solid and global conclusions on trends and chemical markers within the groups of samples more easily and efficiently than more conventional approaches.
2306-5710
http://hdl.handle.net/2445/185884
718781
Characterization of Musts, Wines, and Sparkling Wines Based on Their Elemental Composition Determined by ICP-OES and ICP-MS
oai:diposit.ub.edu:2445/1858852024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Vidal-Casanella, Oscar
author
Arias-Alpizar, Kevin
author
Núñez Burcio, Oscar
author
Saurina, Javier
author
2022-03-28
Flavanols are a class of polyphenols that have become the focus of much research because of their medicinal properties. For this paper, a new method to determine polyphenols in nutraceutical samples was developed, based on solid-liquid extraction and further analysis by liquid chromatography. Analytes were recovered by ultrasound-assisted extraction, using a mixture of methanol/water/hydrochloric acid (70:29:1, v:v:v) as the solvent. Reversed-phase high-performance liquid chromatography with ultraviolet/visible (HPLC-UV/Vis) and fluorescence detection (HPLC-FLD) were developed to determine the content of the principal flavanols. The HPLC-FLD method showed better selectivity and sensitivity, so it was selected for further studies. The analytical parameters established with FLD were satisfactory in terms of linearity (R2 ≥ 0.993), repeatability (RSD% ≥ 2.2), and limits of detection of 0.1 mg L−1 or below. The nutraceutical samples were analyzed by HPLC-FLD to determine the compositional profiles of flavanols, then they were subsequently treated using chemometric methods, such as principal component analysis (PCA). Results showed that the study of compositional profiles from the selected flavanols was sufficient for description and discrimination purposes. The cranberry samples were the richest in flavanols, procyanidin A2 being the principal flavanol, and artichoke samples the least rich.
2297-8739
http://hdl.handle.net/2445/185885
722581
Extraction and characterization of flavanol-rich nutraceuticals based on high-performance liquid chromatography
oai:diposit.ub.edu:2445/1860482024-03-12T17:02:13Zcom_2445_67490com_2445_7181com_2445_7209com_2445_96580col_2445_67491col_2445_7210col_2445_108487
00925njm 22002777a 4500
dc
Lanuza Rilling, Fabián Ignacio
author
Bondonno, Nicola P.
author
Zamora-Ros, Raul
author
Rostgaard Hansen, Agnetha Linn
author
Tjønneland, Anne
author
Landberg, Rikard
author
Halkjær, Jytte
author
Andrés Lacueva, Ma. Cristina
author
2022-04-04
Flavonoids are bioactive plant compounds that are widely present in the human diet. Estimating flavonoid intake with a high degree of certainty is challenging due to the inherent limitations of dietary questionnaires and food composition databases. This study aimed to evaluate the degree of reliability among flavonoid intakes estimated using four different approaches based on the two most comprehensive flavonoid databases, namely, United States Department of Agriculture (USDA) and Phenol Explorer (PE). In 678 individuals from the MAX study, a subcohort of the Diet, Cancer and Health-Next Generations cohort, dietary data were collected using three 24-h diet recalls over 1 year. Estimates of flavonoid intake were compared using flavonoid food content from PE as (1) aglycones (chromatography with hydrolysis), (2) aglycones transformed (converted from glycosides by chromatography without hydrolysis), (3) as they are in nature (glycosides, aglycones, and esters), and 4) using flavonoid content from USDA as aglycones (converted). Spearman's intra-class correlation (ICC) coefficient and weighted kappa (K) coefficient were calculated for the reliability analysis. When comparing PE total aglycones to USDA total aglycones, there was a moderate reliability when a continuous variable was used [ICC: 0.73, 95% confidence interval (CI): 0.70-0.76] and an excellent reliability when flavonoid intake was modeled as a categorical variable (K: 0.89, 95% CI: 0.88-0.90). The degree of reliability among all methods of estimated flavonoid intakes was very similar, especially between database pairs, for the flavanol subclass, while larger differences were observed for flavone, flavonol, and isoflavone subclasses. Our findings indicate that caution should be taken when comparing the results of the associations between flavonoid intakes and health outcomes from studies, when flavonoid intakes were estimated using different methods, particularly for some subclasses.
2296-861X
http://hdl.handle.net/2445/186048
723180
35445059
Comparison of Flavonoid Intake Assessment Methods Using USDA and Phenol Explorer Databases: Subcohort Diet, Cancer and Health-Next GenerationsMAX Study
oai:diposit.ub.edu:2445/1889332024-03-12T17:02:13Zcom_2445_7209com_2445_7181com_2445_7982com_2445_67490com_2445_96580com_2445_107205col_2445_7210col_2445_7983col_2445_67491col_2445_108487col_2445_107206
00925njm 22002777a 4500
dc
Marhuenda-Muñoz, María
author
Domínguez López, Inés
author
Laveriano Santos, Emily P.
author
Parilli Moser, Isabella
author
Razquin, Cristina
author
Ruiz Canela, Miguel
author
Basterra Gortari, F. Javier
author
Corella Piquer, Dolores
author
Salas Salvadó, Jordi
author
Fitó Colomer, Montserrat
author
Lapetra, José
author
Arós, Fernando
author
Fiol Sala, Miguel
author
Serra Majem, Lluís
author
Pintó Sala, Xavier
author
Gómez Gracia, Enrique
author
Ros Rahola, Emilio
author
Estruch Riba, Ramon
author
Lamuela Raventós, Rosa Ma.
author
2022-08-08
The intake of polyphenols has been associated with a risk reduction of type 2 diabetes. Nevertheless, to the best of our knowledge, the molecules that might be metabolically active after ingestion are only starting to be investigated regarding this metabolic disease. To investigate the association between one-year changes in urinary microbial phenolic metabolites (MPM) and the incidence of type 2 diabetes, we performed a case-control study using data and samples of the PREDIMED trial including 46 incident type 2 diabetes cases of 172 randomly selected participants. Eight urinary MPMs were quantified in urine by liquid chromatography coupled to mass spectrometry and used to assess their associations with type 2 diabetes risk by multivariable logistic regression models. Compared to participants in the lowest tertile of one-year changes in hydroxybenzoic acid glucuronide, those in the highest tertile had a significantly lowered probability of developing type 2 diabetes (OR [95% CI], 0.39 [0.23-0.64]; p < 0.001 for trend). However, when additionally adjusting for fasting plasma glucose, the statistical significance was lost. Changes in the dietary pattern can increase the concentrations of this compound, derived from many (poly)phenol-rich foods, and might be changing the gut microbial population as well, promoting the production of the metabolite.
2076-3921
http://hdl.handle.net/2445/188933
36009259
One-Year Changes in Urinary Microbial Phenolic Metabolites and the Risk of Type 2 Diabetes—A Case-Control Study
oai:diposit.ub.edu:2445/1889712024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Núñez, Nerea
author
Pons, Josep
author
Saurina, Javier
author
Núñez Burcio, Oscar
author
2021
Food products are very complex matrices, which makes the quality of these products an issue of great interest in our society. Considering the complexity of the food chain, the adulteration of food is increasing, causing food fraud cases. In this field, drinks are food products that can be very easily adulterated. This work will focus on the thematic of fraud detection in coffee, one of the most popular beverages in the world. Coffee contains an elevated number of bioactive substances (phenolic acids, polyphenols and alkaloids; being especially abundant ellagic, caffeic and chlorogenic acids) that give place to its important antioxidant activity, known for its beneficial health effects. The aim of this work was to develop an efficient non-targeted FIA-ESI-MS fingerprinting method in combination with chemometrics to achieve the characterization, classification, and authentication of coffee samples, together with possible adulterants (barley, chicory and flours) using partial least squares regression-discriminant analysis (PLS-DA) chemometric method. Besides, Arabica and Robusta coffee samples were adulterated with barley, chicory and flour and the obtained FIA-ESI-MS data subjected to partial least squares (PLS) regression. Results demonstrated the feasibility of the proposed methodology to assess coffee authenticity and to quantify adulteration levels (down to 15%), showing good calibration and prediction errors.
2673-9976
http://hdl.handle.net/2445/188971
724442
FIA-ESI-MS Fingerprinting method with chemometrics for the characterization of adulterated coffee samples
oai:diposit.ub.edu:2445/1889812024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
García-Seval, Víctor
author
Martínez Alfaro, Clàudia
author
Saurina, Javier
author
Núñez Burcio, Oscar
author
Sentellas, Sonia
author
2022
Honey is a highly consumed natural product produced by bees which is susceptible to fraudulent practices, some of them regarding their botanical origin. Two HPLC-UV non-targeted finger-printing approaches were evaluated in this work to address honey characterization, classification, and authentication based on honey botanical variety. The first method used no sample treatment and a universal reversed-phase chromatographic separation. On the contrary, the second method was based on an off-line SPE preconcentration method, optimized for the isolation and extraction of polyphenolic compounds, and a reversed-phase chromatographic separation opti-mized for polyphenols as well.. For the off-line SPE method, the use of HLB (3 mL, 60 mg) car-tridges, and 6 mL of methanol as eluent, allowed to achieve acceptable recoveries for the selected polyphenols. The obtained HPLC-UV fingerprints were subjected to exploratory principal com-ponent analysis (PCA) and classificatory partial least squares-discriminant analysis (PLS-DA) to evaluate their viability as sample chemical descriptors for authentication purposes. Both HPLC-UV fingerprints resulted to be appropriate to discriminate between blossom-honeys and honeydew-honeys. However, superior performance was accomplished with off-line SPE HPLC-UV polyphenolic fingerprints, being able to differentiate among the different blos-som-honey samples under study (orange/lemon blossom, rosemary, thyme, eucalyptus, and heather). In general, this work demonstrated the feasibility of HPLC-UV fingerprints, especially those obtained after off-line SPE polyphenolic isolation and extraction, to be employed as honey chemical descriptors to address the characterization and classification of honey samples according to their botanical origin.
2304-8158
http://hdl.handle.net/2445/188981
724440
Characterization, Classification and Authentication of Spanish Blossom and Honeydew Honeys by non-targeted HPLC-UV and off-line SPE HPLC-UV Polyphenolic Fingerprinting Strategies.
oai:diposit.ub.edu:2445/1889822024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Campmajó Galván, Guillem
author
Saurina, Javier
author
Núñez Burcio, Oscar
author
2021
In recent years, nutraceuticals prepared with cranberry (Vaccinium macrocarpon) have gained special attention because of their beneficial effects on human health (e.g., antioxidant activity and antimicrobial activity against bacteria involved in a wide range of diseases), which are mainly attributed to the high content of specific polyphenols in cranberry. However, these products present a risk of fraud consisting of the total or partial substitution of cranberry extracts with cheaper and more abundant fruit extracts. Therefore, in this study, flow injection analysis coupled with high-resolution mass spectrometry (FIA−HRMS) fingerprinting was proposed as a rapid high-throughput analytical approach to address the classification of berry-based products through chemometrics, focusing on cranberry-based products authentication. Thus, several berry-based natural products (including 18 based on blueberry, 25 on grape, 12 on raspberry, and 28 on cranberry) and 21 cranberry-based nutraceuticals were analyzed. Sample treatment consisted of a simple solid-liquid extraction method, using acetone:water: hydrochloric acid (70:29.9:0.1, v/v/v) as the extracting mix. After both negative and positive electrospray ionization FIA−HRMS sample analysis, raw data were processed with mzMine 2.53 software to obtain the corresponding fingerprints. In this line, four different data matricesincluding negative, positive, low-level data fusion (LLDF), and mid-level data fusion (MLDF) FIA−HRMS fingerprints were then subjected to principal component analysis (PCA) and partial least squares regression-discriminant analysis (PLS-DA) using Solo 8.6 chemometrics software. PCA results allowed the identification of specific sample groups and trends. Subsequently, the complete sample classification was segregated through a classification decision tree consecutive two-input class PLS-DA models leading to excellent assignment accuracies after external validation according to sample botanical origin (independently of the employed data matrix). The poster of this work is provided in the supplementary materials.
2673-9976
http://hdl.handle.net/2445/188982
724443
Berry-based products classification by FIA−HRMS fingerprinting and chemometric analysis
oai:diposit.ub.edu:2445/1889352024-03-12T17:02:13Zcom_2445_20400com_2445_7181com_2445_96580col_2445_20401col_2445_108487
00925njm 22002777a 4500
dc
Pons, Josep
author
Núñez, Nerea
author
Saurina, Javier
author
Núñez Burcio, Oscar
author
2021-10-13
Tea (Camellia sinensis) is one of the most popular beverages, commonly consumed all over the world. Depending on the fermentation process, tea leaves can be categorized into three major groups: unfermented green tea, semifermented Oolong tea, and fully fermented black tea. The latter accounts for over 80% of worldwide production. The quality of tea products is determined by color, freshness, strength, and aroma. Phenolic and polyphenolic components contribute to the color and taste, whereas volatile components are directly related to the aroma. Unfortunately, food fraud is increasing globally. The widespread adulteration is the main concern for commercial functional tea extracts and tea-based nutraceuticals on the market. Especially for powdered extracts, the product quality of functional tea extracts varies highly on the market. The growing demand and interest in functional tea extracts are causing the proliferation of frauds that can seriously affect public health. Chicory, husk of pulses, and cereal starch are non-permitted materials typically employed as adulterants in tea extracts. The aim of this work was to develop an efficient untargeted high-performance liquid chromatography with ultraviolet and fluorescence detection (HPLC-UV-FLD) method in combination with chemometrics to address the characterization, classification, and authentication of tea samples, together with possible adulterants such as chicory extracts. A reversed-phase chromatographic separation was optimised, using a C18 column, and 0.1% formic acid aqueous solution and acetonitrile as the mobile phase components. The proposed methodology was applied to 87 tea samples, differing in variety and production region, and 12 chicory samples. In any case, the sample treatment consisted of sample infusion with hot? water and filtration, and the obtained HPLC-UV-FLD fingerprints were subjected to principal component analysis (PCA) and partial least squares regression-discriminant analysis (PLS-DA) chemometric methods. Perfect discrimination was achieved between different tea varieties and chicory demonstrating that untargeted HPLC-UV-FLD fingerprints can be proposed as good sample chemical descriptors to assess tea authentication and to prevent frauds dealing with adulteration with chicory.
2673-9976
http://hdl.handle.net/2445/188935
724441
Untargeted HPLC-UV-FLD Fingerprinting for the Characterization, Classification, and Authentication of Tea
oai:diposit.ub.edu:2445/1891692024-03-12T17:02:13Zcom_2445_16685com_2445_7181com_2445_96580com_2445_67490col_2445_16686col_2445_108487col_2445_67491
00925njm 22002777a 4500
dc
Torrico, Mauricio
author
Casino, Pablo
author
López Sevilla, Asunción
author
Peiró Sánchez, Sara
author
Ríos Alcolea, Martín
author
Ríos, Santiago
author
Montes López, María Jesús
author
Guillen, Carmen
author
Nardi Ricart, Anna
author
García Montoya, Encarna
author
Asensio, Daniel
author
Marqués Villavecchia, Ana M.
author
Piqué i Clusella, Núria
author
2022-06-19
The detection of Salmonella in food is based on the use of a selective enrichment broth such as Muller-Kauffman Tetrathionate-Novobiocin (MKTTn), in which tetrathionate plays a key role by providing Salmonella with a growth advantage. As sodium tetrathionate is unstable, it is generated in situ by the addition of iodine (Lugol's solution) before seeding. This step is cumbersome as the solution is easily spilled, compromising the performance of the medium and hindering the work of technicians. The aim of this study was to optimize MKTTn broth by generating tetrathionate ex situ through an external reaction between iodine and thiosulphate followed by lyophilization. Quality control procedures were performed to compare the modified and original media, testing pure productivity (enrichment with 50-120 CFU of Salmonella Thyphimurim ATCC 14028 and Salmonella Enteritidis ATCC 13076 and plating on Xylose Lysine Deoxycholate agar, XLD), mixed productivity (50-120 CFU of Salmonella strains and Pseudomonas aeruginosa and Escherichia coli at ≥104 CFU and XLD plating) and selectivity (≥104 CFU of P. aeruginosa and Enterococcus faecalis and plating on Tryptone Casein Soy agar, TSA). The modified MKTTn medium (S/L) performed comparably with the original medium in terms of growth of both Salmonella strains (>300 colonies in XLD), alone or with P. aeruginosa and E. coli. Quantitative assays showed no statistically significant differences in the number of colonies grown on XLD after 10-5 dilution (p=0.7015 with S. Thyphimurim ATCC 14028 and p=0.2387 with S. Enteritidis ATCC 13076; ANOVA test). MKTTn medium (S/L) was also selective against E. coli (≤100 colonies) and E. faecalis (<10 colonies). These results suggest that adding tetrathionate as a lyophilisate (S/L) is a feasible alternative to the use of Lugol's solution for the preparation of MKTTn enrichment broth and does not affect the properties of the medium
0167-7012
http://hdl.handle.net/2445/189169
723861
Improvement of Mueller-Kauffman Tetrathionate-Novobiocin (MKTTn) enrichment medium for the detection of Salmonella enterica by the addition of wx situ-generated tetrathionate
oai:diposit.ub.edu:2445/1896172024-03-12T17:02:13Zcom_2445_96580com_2445_7181com_2445_20400col_2445_108487col_2445_20401
00925njm 22002777a 4500
dc
Vilà Romeu, Mònica
author
Bedmar Chamarro, Àlex
author
Saurina, Javier
author
Núñez Burcio, Oscar
author
Sentellas, Sonia
author
2022
Tea is a broadly consumed beverage worldwide that is susceptible to fraudulent practices, in-cluding its adulteration with other plants such as chicory extracts. In the present work, a non-targeted high-throughput flow injection analysis-mass spectrometry (FIA-MS) fingerprint-ing methodology was employed to characterize and classify different varieties of tea (black, green, red, oolong, and white) and chicory extracts by principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). Detection and quantitation of frauds in black and green tea extracts adulterated with chicory were also evaluated as proofs of concept using partial least squares (PLS) regression. Overall, PLS-DA showed that FIA-MS fingerprints in both negative and positive ionization modes were excellent sample chemical descriptors to dis-criminate tea samples from chicory independently of the tea product variety, as well as to clas-sify and discriminate among some of the analyzed tea groups. The classification rate was 100% in all the paired cases ¿i.e., each tea product variety versus chicory¿ by PLS-DA calibration and prediction models showing their capability to assess tea authentication. The results obtained for chicory adulteration detection and quantitation using PLS were satisfactory in the two adultera-tion cases evaluated (green and black teas adulterated with chicory), with calibration, cross-validation, and prediction errors bellow 5.8%, 8.5%, and 16.4%, respectively. Thus, the non-targeted FIA-MS fingerprinting methodology demonstrated to be a high-throughput, cost-effective, simple, and reliable approach to assess tea authentication issues.
2304-8158
http://hdl.handle.net/2445/189617
724274
High-throughput Flow Injection Analysis-Mass Spectrometry (FIA-MS) Fingerprinting for the Authentication of Tea. Application to the Detection of Teas Adulterated with Chicory.
marc///col_2445_108487/100