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DC Field | Value | Language |
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dc.contributor.author | Fernàndez Castillo, Noèlia | - |
dc.contributor.author | Cabana Domínguez, Judit | - |
dc.contributor.author | Soriano i Fradera, Jordi | - |
dc.contributor.author | Sánchez Mora, Cristina | - |
dc.contributor.author | Roncero, Carlos | - |
dc.contributor.author | Grau-López, Lara | - |
dc.contributor.author | Ros Cucurull, Elena | - |
dc.contributor.author | Daigre, Constanza | - |
dc.contributor.author | van Donkelaar, M. M. J. | - |
dc.contributor.author | Franke, B. | - |
dc.contributor.author | Casas, Miquel | - |
dc.contributor.author | Ribasés Haro, Marta | - |
dc.contributor.author | Cormand Rifà, Bru | - |
dc.date.accessioned | 2016-07-14T14:22:52Z | - |
dc.date.available | 2016-07-14T14:22:52Z | - |
dc.date.issued | 2015-10-27 | - |
dc.identifier.issn | 2158-3188 | - |
dc.identifier.uri | http://hdl.handle.net/2445/100508 | - |
dc.description.abstract | Cocaine reward and reinforcing effects are mediated mainly by dopaminergic neurotransmission. In this study, we aimed at evaluating gene expression changes induced by acute cocaine exposure on SH-SY5Y-differentiated cells, which have been widely used as a dopaminergic neuronal model. Expression changes and a concomitant increase in neuronal activity were observed after a 5 μM cocaine exposure, whereas no changes in gene expression or in neuronal activity took place at 1 μM cocaine. Changes in gene expression were identified in a total of 756 genes, mainly related to regulation of transcription and gene expression, cell cycle, adhesion and cell projection, as well as mitogen-activeated protein kinase (MAPK), CREB, neurotrophin and neuregulin signaling pathways. Some genes displaying altered expression were subsequently targeted with predicted functional single-nucleotide polymorphisms (SNPs) in a case-control association study in a sample of 806 cocaine-dependent patients and 817 controls. This study highlighted associations between cocaine dependence and five SNPs predicted to alter microRNA binding at the 3′-untranslated region of the NFAT5 gene. The association of SNP rs1437134 with cocaine dependence survived the Bonferroni correction for multiple testing. A functional effect was confirmed for this variant by a luciferase reporter assay, with lower expression observed for the rs1437134G allele, which was more pronounced in the presence of hsa-miR-509. However, brain volumes in regions of relevance to addiction, as assessed with magnetic resonance imaging, did not correlate with NFAT5 variation. These results suggest that the NFAT5 gene, which is upregulated a few hours after cocaine exposure, may be involved in the genetic predisposition to cocaine dependence. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1038/tp.2015.158 | - |
dc.relation.ispartof | Translational Psychiatry, 2015, vol. 5, num. e667 | - |
dc.relation.uri | http://dx.doi.org/10.1038/tp.2015.158 | - |
dc.rights | cc-by-nc-nd (c) Fernàndez-Castillo, N. et al., 2015 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Cocaïna | - |
dc.subject.classification | Expressió gènica | - |
dc.subject.classification | Proteïnes quinases | - |
dc.subject.other | Cocaine | - |
dc.subject.other | Gene expression | - |
dc.subject.other | Protein kinases | - |
dc.title | Transcriptomic and genetic studies identify NFAT5 as a candidate gene for cocaine dependence | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 654128 | - |
dc.date.updated | 2016-07-14T14:22:57Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/643051/EU//MiND | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/602450/EU//IMAGEMEND | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/602805/EU//AGGRESSOTYPE | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 26506053 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) Publicacions de projectes de recerca finançats per la UE |
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654128.pdf | 1.1 MB | Adobe PDF | View/Open |
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