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Title: Characterisation of extended-spectrum b-lactamases among Klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in Mozambique
Author: Pons, Maria J.
Vubil, Delfino
Guiral, Elisabet
Jaintilal, Dinis
Fraile, Oscar
Soto, Sara M. (Sara Maria)
Sigaúque, Betuel
Nhampossa, Tacilta
Aide, Pedro Carlos Paulino
Alonso, Pedro
Vila Estapé, Jordi
Mandomando, Inácio
Ruiz, Joaquim
Keywords: Klebsiella pneumoniae
Infeccions del tracte urinari
Klebsiella pneumoniae
Urinary tract infections
Issue Date: 7-Jan-2015
Publisher: Elsevier
Abstract: The aim of this study was to determine the prevalence of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolated from urinary tract and bloodstream infections in a rural hospital in Manhiça, Mozambique. ESBLs were investigated among ceftriaxone-non-susceptible K. pneumoniae clinical isolates recovered between 2004 and 2009. Characterisation of blaCTX-M, blaSHV, blaOXA and blaTEM genes was performed by PCR and sequencing. Epidemiological relationships were established by phylogenetic analysis, repetitive extragenic palindromic PCR (REP-PCR), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), whilst plasmid transferability was evaluated by conjugation. In addition, the presence of class 1 and 2 integrons was studied. A total of 19 K. pneumoniae were analysed. The blaCTX-M-15 gene was found in all strains. Other ESBL genes were found concomitantly, including blaSHV-5, blaSHV-2, blaSHV-2A, blaSHV-12 and blaSHV-38. In addition, other β-lactamases such as blaTEM-1 and blaOXA-30 were also detected. REP-PCR identified 15 different epidemiological profiles. MLST analysis also showed great variability of sequence types. The blaCTX-M-15 gene showed a high transfer capacity. The presence of class 1 integrons was high. High levels of multidrug resistance were also found. In conclusion, these data show the dominance of the CTX-M-type ESBL, particularly CTX-M-15, supporting its worldwide dissemination, including in areas with limited access to third-generation cephalosporins. This finding is a matter of concern for clinical management as third-generation cephalosporins are an alternative for treating severe cases of multidrug-resistant infections in this community.
Note: Versió postprint del document publicat a:
It is part of: Journal of Global Antimicrobial Resistance, 2015, vol. 3, num. 1, p. 19-25
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ISSN: 2213-7165
Appears in Collections:Articles publicats en revistes (ISGlobal)

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