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DC Field | Value | Language |
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dc.contributor.author | Pons, Maria J. | - |
dc.contributor.author | Vubil, Delfino | - |
dc.contributor.author | Guiral Vilalta, Elisabet | - |
dc.contributor.author | Jaintilal, Dinis | - |
dc.contributor.author | Fraile, Oscar | - |
dc.contributor.author | Soto González, Sara M. | - |
dc.contributor.author | Sigaúque, Betuel | - |
dc.contributor.author | Nhampossa, Tacilta | - |
dc.contributor.author | Aide, Pedro Carlos Paulino | - |
dc.contributor.author | Alonso, Pedro | - |
dc.contributor.author | Vila Estapé, Jordi | - |
dc.contributor.author | Mandomando, Inácio | - |
dc.contributor.author | Ruiz, Joaquim | - |
dc.date.accessioned | 2016-07-18T08:13:47Z | - |
dc.date.available | 2016-07-18T08:13:47Z | - |
dc.date.issued | 2015-01-07 | - |
dc.identifier.issn | 2213-7165 | - |
dc.identifier.uri | http://hdl.handle.net/2445/100567 | - |
dc.description.abstract | The aim of this study was to determine the prevalence of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolated from urinary tract and bloodstream infections in a rural hospital in Manhiça, Mozambique. ESBLs were investigated among ceftriaxone-non-susceptible K. pneumoniae clinical isolates recovered between 2004 and 2009. Characterisation of blaCTX-M, blaSHV, blaOXA and blaTEM genes was performed by PCR and sequencing. Epidemiological relationships were established by phylogenetic analysis, repetitive extragenic palindromic PCR (REP-PCR), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), whilst plasmid transferability was evaluated by conjugation. In addition, the presence of class 1 and 2 integrons was studied. A total of 19 K. pneumoniae were analysed. The blaCTX-M-15 gene was found in all strains. Other ESBL genes were found concomitantly, including blaSHV-5, blaSHV-2, blaSHV-2A, blaSHV-12 and blaSHV-38. In addition, other β-lactamases such as blaTEM-1 and blaOXA-30 were also detected. REP-PCR identified 15 different epidemiological profiles. MLST analysis also showed great variability of sequence types. The blaCTX-M-15 gene showed a high transfer capacity. The presence of class 1 integrons was high. High levels of multidrug resistance were also found. In conclusion, these data show the dominance of the CTX-M-type ESBL, particularly CTX-M-15, supporting its worldwide dissemination, including in areas with limited access to third-generation cephalosporins. This finding is a matter of concern for clinical management as third-generation cephalosporins are an alternative for treating severe cases of multidrug-resistant infections in this community. | - |
dc.format.extent | 33 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.jgar.2015.01.004 | - |
dc.relation.ispartof | Journal of Global Antimicrobial Resistance, 2015, vol. 3, num. 1, p. 19-25 | - |
dc.relation.uri | http://dx.doi.org/10.1016/j.jgar.2015.01.004 | - |
dc.rights | cc by-nc-nd (c) Elsevier, 2015 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | - |
dc.source | Articles publicats en revistes (ISGlobal) | - |
dc.subject.classification | Klebsiella pneumoniae | - |
dc.subject.classification | Infeccions del tracte urinari | - |
dc.subject.classification | Moçambic | - |
dc.subject.other | Klebsiella pneumoniae | - |
dc.subject.other | Urinary tract infections | - |
dc.subject.other | Mozambique | - |
dc.title | Characterisation of extended-spectrum b-lactamases among Klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in Mozambique | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.date.updated | 2016-07-01T10:21:39Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
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File | Description | Size | Format | |
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pons2015_2022.pdf | 351.27 kB | Adobe PDF | View/Open |
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