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DC Field | Value | Language |
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dc.contributor.author | Coll, Pere | - |
dc.contributor.author | Aragón, Lina Marcela | - |
dc.contributor.author | Alcaide Fernández de Vega, Fernando | - |
dc.contributor.author | Espasa, Mateo | - |
dc.contributor.author | Garrigó, Montserrat | - |
dc.contributor.author | González Martín, Julián | - |
dc.contributor.author | Manterola, Jose M. | - |
dc.contributor.author | Orús, Pilar | - |
dc.contributor.author | Salvadó, Margarita | - |
dc.date.accessioned | 2016-07-26T06:38:52Z | - |
dc.date.available | 2016-07-26T06:38:52Z | - |
dc.date.issued | 2005-05-23 | - |
dc.identifier.issn | 1076-6294 | - |
dc.identifier.uri | http://hdl.handle.net/2445/101000 | - |
dc.description.abstract | We studied the presence of mutations in the whole katG gene and specific regions of the oxyR-ahpC and mabA-inhA regulatory region in 61 Mycobacterium tuberculosis isoniazid-resistant isolates. An 81-bp region of the rpoB gene was also sequenced in 17 rifampin-resistant strains. Alterations in the katG gene were detected in 55% of the isolates. Mutation in codon 315 was the most prevalent (32%). Strains showed a high level of resistance, and most maintained a substantial catalase-peroxidase activity. Three strains with an isoniazid MIC of ≥32 µg/ml lacked catalase-peroxidase activity. Two of them had deletions in the catalytic domain of the KatG protein. One strain with deletion and three strains with mutations in the C-terminal domain showed low-level resistance and conserved the catalase-peroxidase activity. Mutations in the mabA-inhA regulatory region were identified in 32% of the isolates. All had low-level resistance, and the vast majority conserved catalase-peroxidase activity. Seventeen percent of the isoniazid-resistant isolates had no detectable alterations at the studied loci. Resistance to rifampin was associated with mutations in the 81-bp of the rpoB gene in all cases. IS6110 analysis indicated that recent transmission contributed substantially to the emergence of isoniazid- resistant tuberculosis in Barcelona through short transmission chains. A rapid genotypic assay, including the 315-katG codon and the -15 nucleotide of the mabA-inhA regulatory region, may cover 62% of isoniazid- resistant strains in Barcelona. In contrast, the targeting of the 81-bp region of rpoB would detect all our rifampin-resistant isolates. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Mary Ann Liebert, Inc. | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1089/mdr.2005.11.107 | - |
dc.relation.ispartof | Microbial Drug Resistance, 2005, vol. 11, num. 2, p. 107-114 | - |
dc.relation.uri | http://dx.doi.org/10.1089/mdr.2005.11.107 | - |
dc.rights | (c) Mary Ann Liebert, Inc., 2005 | - |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Mycobacterium tuberculosis | - |
dc.subject.classification | Tuberculosi | - |
dc.subject.classification | Medicaments antituberculosos | - |
dc.subject.classification | Resistència als medicaments | - |
dc.subject.classification | Genètica molecular | - |
dc.subject.other | Mycobacterium tuberculosis | - |
dc.subject.other | Tuberculosis | - |
dc.subject.other | Antitubercular agents | - |
dc.subject.other | Drug resistance | - |
dc.subject.other | Molecular genetics | - |
dc.title | Molecular analysis of isoniazid and rifampin resistance in Mycobacterium tuberculosis isolates recovered from Barcelona. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 639143 | - |
dc.date.updated | 2016-07-26T06:38:58Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 15910223 | - |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) |
Files in This Item:
File | Description | Size | Format | |
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639143.pdf | 89.44 kB | Adobe PDF | View/Open |
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