Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/101324
Title: Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes
Author: Calderon-Dominguez, María
Sebastián Muñoz, David
Fucho Salvador, Raquel
Weber Blattes, Minéia
Mir Bonnín, Joan Francesc
García-Casarrubios, Ester
Obregón, Maria Jesús
Zorzano Olarte, Antonio
Valverde, Ángela M.
Serra i Cucurull, Dolors
Herrero Rodríguez, Laura
Keywords: Carnitina palmitoïl-transferasa 1
Teixit adipós
Obesitat
Carnitine palmitoyltransferase I
Adipose tissues
Obesity
Issue Date: 20-Jul-2016
Publisher: Public Library of Science (PLoS)
Abstract: The discovery of active brown adipose tissue (BAT) in adult humans and the fact that it is reduced in obese and diabetic patients have put a spotlight on this tissue as a key player in obesity-induced metabolic disorders. BAT regulates energy expenditure through thermogenesis; therefore, harnessing its thermogenic fat-burning power is an attractive therapeutic approach. We aimed to enhance BAT thermogenesis by increasing its fatty acid oxidation (FAO) rate. Thus, we expressed carnitine palmitoyltransferase 1AM (CPT1AM), a permanently active mutant form of CPT1A (the rate-limiting enzyme in FAO), in a rat brown adipocyte (rBA) cell line through adenoviral infection. We found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements. This indicates that CPT1AM-mediated increase in brown adipocytes FAO may be a new approach to the treatment of obesity-induced disorders.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0159399
It is part of: PLoS One, 2016, vol. 11, num. 7, p. e0159399
Related resource: http://dx.doi.org/10.1371/journal.pone.0159399
URI: http://hdl.handle.net/2445/101324
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)
Articles publicats en revistes (Institut de Biomedicina (IBUB))
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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