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http://hdl.handle.net/2445/101324
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DC Field | Value | Language |
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dc.contributor.author | Calderón Domínguez, María | - |
dc.contributor.author | Sebastián Muñoz, David | - |
dc.contributor.author | Fucho Salvador, Raquel | - |
dc.contributor.author | Weber Blattes, Minéia | - |
dc.contributor.author | Mir Bonnín, Joan Francesc | - |
dc.contributor.author | García-Casarrubios, Ester | - |
dc.contributor.author | Obregón, Maria Jesús | - |
dc.contributor.author | Zorzano Olarte, Antonio | - |
dc.contributor.author | Valverde, Ángela M. | - |
dc.contributor.author | Serra i Cucurull, Dolors | - |
dc.contributor.author | Herrero Rodríguez, Laura | - |
dc.date.accessioned | 2016-07-27T09:30:29Z | - |
dc.date.available | 2016-07-27T09:30:29Z | - |
dc.date.issued | 2016-07-20 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/101324 | - |
dc.description.abstract | The discovery of active brown adipose tissue (BAT) in adult humans and the fact that it is reduced in obese and diabetic patients have put a spotlight on this tissue as a key player in obesity-induced metabolic disorders. BAT regulates energy expenditure through thermogenesis; therefore, harnessing its thermogenic fat-burning power is an attractive therapeutic approach. We aimed to enhance BAT thermogenesis by increasing its fatty acid oxidation (FAO) rate. Thus, we expressed carnitine palmitoyltransferase 1AM (CPT1AM), a permanently active mutant form of CPT1A (the rate-limiting enzyme in FAO), in a rat brown adipocyte (rBA) cell line through adenoviral infection. We found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements. This indicates that CPT1AM-mediated increase in brown adipocytes FAO may be a new approach to the treatment of obesity-induced disorders. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0159399 | - |
dc.relation.ispartof | PLoS One, 2016, vol. 11, num. 7, p. e0159399 | - |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pone.0159399 | - |
dc.rights | cc-by (c) Calderon-Dominguez, María et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | Carnitina palmitoïl-transferasa 1 | - |
dc.subject.classification | Teixit adipós | - |
dc.subject.classification | Obesitat | - |
dc.subject.other | Carnitine palmitoyltransferase I | - |
dc.subject.other | Adipose tissues | - |
dc.subject.other | Obesity | - |
dc.title | Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 663289 | - |
dc.date.updated | 2016-07-27T09:30:34Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27438137 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) Articles publicats en revistes (Institut de Biomedicina (IBUB)) Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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663289.pdf | 1.61 MB | Adobe PDF | View/Open |
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