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http://hdl.handle.net/2445/101543
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DC Field | Value | Language |
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dc.contributor.author | Martínez Huélamo, Miriam | - |
dc.contributor.author | Tulipani, Sara | - |
dc.contributor.author | Jáuregui Pallarés, Olga | - |
dc.contributor.author | Valderas Martínez, Palmira | - |
dc.contributor.author | Vallverdú i Queralt, Anna | - |
dc.contributor.author | Estruch Riba, Ramon | - |
dc.contributor.author | Torrado, Xavier | - |
dc.contributor.author | Lamuela Raventós, Rosa Ma. | - |
dc.date.accessioned | 2016-09-05T12:40:17Z | - |
dc.date.available | 2016-09-05T12:40:17Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | http://hdl.handle.net/2445/101543 | - |
dc.description.abstract | An UHPLC-MS/MS method for the quantification of tomato phenolic metabolites in human fluids was optimized and validated, and then applied in a pilot dietary intervention study with healthy volunteers. A 5-fold gain in speed (3.5 min of total run); 7-fold increase in MS sensitivity and 2-fold greater efficiency (50% peak width reduction) were observed when comparing the proposed method with the reference-quality HPLC-MS/MS system, whose assay performance has been previously documented. The UHPLC-MS/MS method led to an overall improvement in the limits of detection (LOD) and quantification (LOQ) for all the phenolic compounds studied. The recoveries ranged between 68% and 100% in urine and 61% and 100% in plasma. The accuracy; intra- and interday precision; and stability met with the acceptance criteria of the AOAC International norms. Due to the improvements in the analytical method; the total phenolic metabolites detected in plasma and urine in the pilot intervention study were 3 times higher than those detected by HPLC-MS/MS. Comparing with traditional methods; which require longer time of analysis; the methodology described is suitable for the analysis of phenolic compounds in a large number of plasma and urine samples in a reduced time frame. | - |
dc.format.extent | 17 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.3390/molecules201119702 | - |
dc.relation.ispartof | Molecules, 2015, vol. 20, num. 11, p. 20409-20425 | - |
dc.relation.uri | http://dx.doi.org/10.3390/molecules201119702 | - |
dc.rights | cc-by (c) Martínez-Huélamo, M. et al., 2015 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) | - |
dc.subject.classification | Biologia humana | - |
dc.subject.classification | Tomàquets | - |
dc.subject.classification | Metabòlits | - |
dc.subject.other | Human biology | - |
dc.subject.other | Tomatoes | - |
dc.subject.other | Metabolites | - |
dc.title | Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 655355 | - |
dc.date.updated | 2016-09-05T12:40:22Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 26580589 | - |
Appears in Collections: | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) |
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655355.pdf | 798.82 kB | Adobe PDF | View/Open |
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