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Title: A novel Plasmodium falciparum rhoptry associated adhesin mediates erythrocyte invasion through the sialic-acid dependent pathway
Author: Anand, Gaurav
Reddy, K. Sony
Pandey, Alok Kumar
Mian, Syed Yusuf
Singh, Hina
Mittal, Shivani Arora
Amlabu, Emmanuel
Bassat Orellana, Quique
Mayor Aparicio, Alfredo Gabriel
Chauhan, Virander Singh
Gaur, Deepak
Keywords: Plasmodium falciparum
Plasmodium falciparum
Issue Date: 7-Jul-2016
Publisher: Nature
Abstract: Erythrocyte invasion by Plasmodium falciparum merozoites is central to blood-stage infection and malaria pathogenesis. This intricate process is coordinated by multiple parasite adhesins that bind erythrocyte receptors and mediate invasion through several alternate pathways. P. falciparum expresses 2700 genes during the blood-stages, of which the identity and function of many remains unknown. Here, we have identified and characterized a novel P. falciparum rhoptry associated adhesin (PfRA) that mediates erythrocyte invasion through the sialic-acid dependent pathway. PfRA appears to play a significant functional role as it is conserved across different Plasmodium species. It is localized in the rhoptries and further translocated to the merozoite surface. Both native and recombinant PfRA specifically bound erythrocytes in a sialic-acid dependent, chymotrypsin and trypsin resistant manner, which was abrogated by PfRA antibodies confirming a role in erythrocyte invasion. PfRA antibodies inhibited erythrocyte invasion and in combination with antibodies against other parasite ligands produced an additive inhibitory effect, thus validating its important role in erythrocyte invasion. We have thus identified a novel P. falciparum adhesin that binds with a sialic acid containing erythrocyte receptor. Our observations substantiate the strategy to block P. falciparum erythrocyte invasion by simultaneously targeting multiple conserved merozoite antigens involved in alternate invasion pathways.
Note: Reproducció del document publicat a:
It is part of: Scientific Reports, 2016, vol. 6, num. 29185, p. 1-17
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (ISGlobal)

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