Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/101986
Title: HPLC fingerprints for the authentication of cranberry-based products based on multivariate calibration approaches
Author: Puigventós, Lídia
Núñez Burcio, Oscar
Saurina, Javier
Keywords: Cromatografia de líquids
Quimiometria
Polifenols
Química dels aliments
Liquid chromatography
Chemometrics
Polyphenols
Food composition
Issue Date: 2016
Publisher: Bentham Science Publishers
Abstract: This work introduces the topic of the authentication of cranberry-based products and the detection and quantification of possible adulterations with other raw materials of lower quality. For such a purpose, genuine and adulterated cranberry samples were analyzed by reversed-phase HPLC with UV detection. Sample components were separated using an elution gradient based on 0.1% (v/v) formic acid aqueous solution and methanol as the components of the mobile phase. Chromatograms were recorded at 280, 370 and 520 nm. Data resulting from the injection of pure and adulterated samples, consisting of chromatographic fingerprints at each detection wavelength, were analyzed chemometrically. Preliminary studies by Principal Component Analysis showed that the sample extracts were clearly distributed depending on the extent of adulteration. Data was further treated by Partial Least Square regression to determine the percentages of grape contamination. It was found that even mixture samples containing low percentages of grape could be distinguished from genuine cranberry extracts. Besides, results obtained were highly satisfactory, with overall quantification errors lower than 5%. As a conclusion, the method proposed here resulted in an excellent approach to carry out the authentication of cranberry-based products relying on polyphenolic fingerprints.
Note: Versió postprint del document publicat a: http://dx.doi.org/10.2174/1573411012666160216220526
It is part of: Current Analytical Chemistry, 2016, vol. 12
Related resource: http://dx.doi.org/10.2174/1573411012666160216220526
URI: http://hdl.handle.net/2445/101986
ISSN: 1573-4110
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

Files in This Item:
File Description SizeFormat 
657139.pdf244.27 kBAdobe PDFView/Open    Request a copy


Embargat   Document embargat fins el 31-12-2017


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.