Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/102127
Title: Endocrine-Therapy-Resistant ESR1 Variants Revealed by Genomic Characterization of Breast-Cancer-Derived Xenografts
Author: Li, S.
Shen, D.
Shao, J.
Crowder, R.
Liu, W.
Prat Aparicio, Aleix
He, X.
Liu, S.
Hoog, J.
Lu, C.
Ding, L.
Griffith, O.L.
Miller, C.
Larson, D.
Fulton, R.S.
Harrison, M.
Mooney, T.
McMichael, J.F.
Luo, J.
Tao, Y.
Goncalves, R.
Schlosberg, C.
Hiken, J.F.
Saied, L.
Sanchez, C.
Giuntoli, T.
Bumb, C.
Cooper, C.
Kitchens, R.T.
Lin, A.
Phommaly, C.
Davies, S.R.
Zhang, J.
Kavuri, M.S.
McEachern, D.
Dong, Y.Y.
Ma, C.
Pluard, T.
Naughton, M.
Bose, R.
Keywords: Càncer de mama
Genòmica
Biologia molecular
Estudi de casos
Breast cancer
Genomics
Molecular biology
Case studies
Issue Date: 19-Sep-2013
Publisher: Elsevier
Abstract: To characterize patient-derived xenografts (PDXs) for functional studies, we made whole-genome comparisons with originating breast cancers representative of the major intrinsic subtypes. Structural and copy number aberrations were found to be retained with high fidelity. However, at the single-nucleotide level, variable numbers of PDX-specific somatic events were documented, although they were only rarely functionally significant. Variant allele frequencies were often preserved in the PDXs, demonstrating that clonal representation can be transplantable. Estrogen-receptor-positive PDXs were associated with ESR1 ligand-binding-domain mutations, gene amplification, or an ESR1/YAP1 translocation. These events produced different endocrine-therapy-response phenotypes in human, cell line, and PDX endocrine-response studies. Hence, deeply sequenced PDX models are an important resource for the search for genome-forward treatment options and capture endocrine-drug-resistance etiologies that are not observed in standard cell lines. The originating tumor genome provides a benchmark for assessing genetic drift and clonal representation after transplantation.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1016/j.celrep.2013.08.022
It is part of: Cell Reports, 2013, vol. 4, num. 6, p. 1116-1130
Related resource: http://dx.doi.org/10.1016/j.celrep.2013.08.022
URI: http://hdl.handle.net/2445/102127
ISSN: 2211-1247
Appears in Collections:Articles publicats en revistes (Medicina)

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