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DC Field | Value | Language |
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dc.contributor.author | Gomes, Cláudia | - |
dc.contributor.author | Palma, Noemí | - |
dc.contributor.author | Pons, Maria J. | - |
dc.contributor.author | Magallón Tejada, Ariel | - |
dc.contributor.author | Sandoval, Isabel | - |
dc.contributor.author | Tinco-Valdez, Carmen | - |
dc.contributor.author | Gutarra, Carlos | - |
dc.contributor.author | Valle Mendoza, Juana del | - |
dc.contributor.author | Ruiz, Joaquim | - |
dc.contributor.author | Matsuoka, Mayumi | - |
dc.date.accessioned | 2016-10-07T14:06:20Z | - |
dc.date.available | 2016-10-07T14:06:20Z | - |
dc.date.issued | 2016-09-14 | - |
dc.identifier.issn | 1935-2727 | - |
dc.identifier.uri | http://hdl.handle.net/2445/102466 | - |
dc.description.abstract | BACKGROUND: Bartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas. METHODOLOGY/PRINCIPAL FINDINGS: Blood (n = 198) and serum (n = 177) samples were collected in northern Peru. Clinical data were recorded. Specific 16S rRNA amplification by RT-PCR, IFA and ELISA for IgM/IgG with whole cells as antigens was done. Western blot analysis and N-terminal amino acid sequencing detected seroreactive proteins. ELISAs for IgM/IgG for the antigenic candidates were performed. Of the population 33.3% reported at least one symptom compatible with Carrion's disease; 25.4% (IFA), 27.1% (ELISA-IgG), 33.9% (ELISA-IgM) and 38.9% (RT-PCR) of samples were positive. Four proteins were considered potential antigenic candidates, including two new antigenic candidates, succinyl-CoA synthetase subunit alpha (SCS-alpha) and succinyl-CoA synthetase subunit beta (SCS-beta). On Western blot both Pap31 and SCS-alpha interacted with IgM, while GroEL and SCS-beta interacted with IgG. The presence of specific antibodies against the antigenic candidates varied from 34.5% (IgG against SCS-alpha) to 97.2% (IgM against Pap31). CONCLUSIONS/SIGNIFICANCE: RT-PCR and the high levels of positivity for specific ELISAs demonstrate high levels of B. bacilliformis exposure and asymptomatic carriers among inhabitants. The new antigens identified might be used as a new rapid diagnostic tool to diagnose acute Carrion's disease and identify asymptomatic carriers. | - |
dc.format.extent | 17 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pntd.0004989 | - |
dc.relation.ispartof | PLoS Neglected Tropical Diseases, 2016, vol. 10, num. 9, p. e0004989 | - |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pntd.0004989 | - |
dc.rights | cc by (c) Gomes et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | - |
dc.source | Articles publicats en revistes (ISGlobal) | - |
dc.subject.classification | Malalties bacterianes | - |
dc.subject.classification | Paisatge rural | - |
dc.subject.classification | Antígens bacterians | - |
dc.subject.other | Bacterial diseases | - |
dc.subject.other | Rural landscape | - |
dc.subject.other | Bacterial antigens | - |
dc.title | Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2016-10-05T18:00:57Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27627803 | - |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
Files in This Item:
File | Description | Size | Format | |
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gomes2016_2244.pdf | 1.38 MB | Adobe PDF | View/Open |
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