Please use this identifier to cite or link to this item:
Title: Emergence of Amoxicillin-Resistant Variants of Spain(9V)-ST156 Pneumococci Expressing Serotype 11A Correlates with Their Ability to Evade the Host Immune Response
Author: Aguinagalde, Leire
Corsini, Bruno
Domenech Pena, Arnau
Domenech, Mirian
Cámara, Jordi
Ardanuy Tisaire, María Carmen
García, Ernesto
Liñares Louzao, Josefina
Fenoll, Asunción
Yuste, Jose
Keywords: Pneumococs
Resistència als medicaments
Streptococcus pneumonia
Drug resistance
Issue Date: 14-Sep-2015
Publisher: Public Library of Science (PLoS)
Abstract: Capsular switching allows pre-existing clones of Streptococcus pneumoniae expressing vaccine serotypes to escape the vaccine-induced immunity by acquisition of capsular genes from pneumococci of a non-vaccine serotype. Here, we have analysed the clonal composition of 492 clinical isolates of serotype 11A causing invasive disease in Spain (2000-2012), and their ability to evade the host immune response. Antibiograms, serotyping and molecular typing were performed. The restriction profiles of pbp2x, pbp1a and pbp2b genes were also analysed. Interaction with the complement components C1q, C3b, C4BP, and factor H was explored whereas opsonophagocytosis assays were performed using a human cell line differentiated to neutrophils. Biofilm formation and the polymorphisms of the major autolysin LytA were evaluated. The main genotypes of the 11A pneumococci were: ST62 (447 isolates, 90.6%), followed by ST6521 (35 isolates, 7.3%) and ST838 (10 isolates, 2.1%). Beta lactam resistant serotype 11A variants of genotypes ST838 and ST6521 closely related to the Spain9V-ST156 clone were first detected in 2005. A different pattern of evasion of complement immunity and phagocytosis was observed between genotypes. The emergence of one vaccine escape variant of Spain9V-ST156 (ST652111A), showing a high potential to avoid the host immune response, was observed. In addition, isolates of ST652111A showed higher ability to produce biofilms than ST83811A or ST6211A, which may have contributed to the emergence of this PEN-resistant ST652111A genotype in the last few years. The emergence of penicillin-resistant 11A invasive variants of the highly successful ST156 clonal complex merits close monitoring.
Note: Reproducció del document publicat a:
It is part of: PLoS One, 2015, vol. 10, num. 9, p. e0137565
Related resource:
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

Files in This Item:
File Description SizeFormat 
662391.pdf2.32 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons