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Title: Cytoadhesion to gC1qR through Plasmodium falciparum Erythrocyte Membrane Protein 1 in Severe Malaria
Author: Magallón Tejada, Ariel
Machevo, Sonia
Cisteró, Pau
Lavstsen, Thomas
Aide, Pedro Carlos Paulino
Rubio, Mercedes
Jiménez, Alfons
Turner, Louise
Valmaseda, Aida
Gupta, Himanshu
Salas, Briege de las
Mandomando, Inácio
Wang, Christian W.
Petersen, Jens E. V
Muñoz, José
Gascón i Brustenga, Joaquim
Macete, Eusébio
Alonso, Pedro
Chitnis, Chetan E.
Bassat Orellana, Quique
Mayor Aparicio, Alfredo Gabriel
Keywords: Malària
Malalties infeccioses
Communicable diseases
Issue Date: 11-Nov-2016
Publisher: Public Library of Science (PLoS)
Abstract: Cytoadhesion of Plasmodium falciparum infected erythrocytes to gC1qR has been associated with severe malaria, but the parasite ligand involved is currently unknown. To assess if binding to gC1qR is mediated through the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family, we analyzed by static binding assays and qPCR the cytoadhesion and var gene transcriptional profile of 86 P. falciparum isolates from Mozambican children with severe and uncomplicated malaria, as well as of a P. falciparum 3D7 line selected for binding to gC1qR (Pf3D7gC1qR). Transcript levels of DC8 correlated positively with cytoadhesion to gC1qR (rho = 0.287, P = 0.007), were higher in isolates from children with severe anemia than with uncomplicated malaria, as well as in isolates from Europeans presenting a first episode of malaria (n = 21) than Mozambican adults (n = 25), and were associated with an increased IgG recognition of infected erythrocytes by flow cytometry. Pf3D7gC1qR overexpressed the DC8 type PFD0020c (5.3-fold transcript levels relative to Seryl-tRNA-synthetase gene) compared to the unselected line (0.001-fold). DBLbeta12 from PFD0020c bound to gC1qR in ELISA-based binding assays and polyclonal antibodies against this domain were able to inhibit binding to gC1qR of Pf3D7gC1qR and four Mozambican P. falciparum isolates by 50%. Our results show that DC8-type PfEMP1s mediate binding to gC1qR through conserved surface epitopes in DBLbeta12 domain which can be inhibited by strain-transcending functional antibodies. This study supports a key role for gC1qR in malaria-associated endovascular pathogenesis and suggests the feasibility of designing interventions against severe malaria targeting this specific interaction.
Note: Reproducció del document publicat a:
It is part of: PLoS Pathogens, 2016, vol. 12, num. 11, p. e1006011
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ISSN: 1553-7366
Appears in Collections:Articles publicats en revistes (ISGlobal)

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