Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/104042
Title: Apoptotic markers in cultured fibroblasts correlate with brain metabolites and regional brain volume in antipsychotic-naïve first-episode schizophrenia and healthy controls
Author: Batalla, A.
Bargalló Alabart, Núria​
Gassó Astorga, Patricia
Molina, O.
Pareto, D.
Mas Herrero, Sergi
Roca, J. M.
Bernardo Arroyo, Miquel
Lafuente, Amàlia, 1952-
Parellada Rodón, Eduard
Keywords: Fibroblasts
Esquizofrènia
Neurotransmissors
Apoptosi
Neuroanatomia
Ressonància magnètica
Fibroblasts
Schizophrenia
Neurotransmitters
Apoptosis
Neuroanatomy
Magnetic resonance
Issue Date: 25-Aug-2015
Publisher: Nature Publishing Group
Abstract: Cultured fibroblasts from first-episode schizophrenia patients (FES) have shown increased susceptibility to apoptosis, which may be related to glutamate dysfunction and progressive neuroanatomical changes. Here we determine whether apoptotic markers obtained from cultured fibroblasts in FES and controls correlate with changes in brain glutamate and N-acetylaspartate (NAA) and regional brain volumes. Eleven antipsychotic-naive FES and seven age- and gender-matched controls underwent 3-Tesla magnetic resonance imaging scanning. Glutamate plus glutamine (Glx) and NAA levels were measured in the anterior cingulate (AC) and the left thalamus (LT). Hallmarks of apoptotic susceptibility (caspase-3-baseline activity, phosphatidylserine externalization and chromatin condensation) were measured in fibroblast cultures obtained from skin biopsies after inducing apoptosis with staurosporine (STS) at doses of 0.25 and 0.5 μM. Apoptotic biomarkers were correlated to brain metabolites and regional brain volume. FES and controls showed a negative correlation in the AC between Glx levels and percentages of cells with condensed chromatin (CC) after both apoptosis inductions (STS 0.5 μM: r = − 0.90; P = 0.001; STS 0.25 μM: r = − 0.73; P = 0.003), and between NAA and cells with CC (STS 0.5 μM induction r = − 0.76; P = 0.002; STS 0.25 μM r = − 0.62; P = 0.01). In addition, we found a negative correlation between percentages of cells with CC and regional brain volume in the right supratemporal cortex and post-central region (STS 0.25 and 0.5 μM; Po0.05 family-wise error corrected (FWEc)). We reveal for the first time that peripheral markers of apoptotic susceptibility may correlate with brain metabolites, Glx and NAA, and regional brain volume in FES and controls, which is consistent with the neuroprogressive theories around the onset of the schizophrenia illness.
Note: Reproducció del document publicat a: https://doi.org/10.1038/tp.2015.122
It is part of: Translational Psychiatry, 2015, vol. 5, p. e626
Related resource: https://doi.org/10.1038/tp.2015.122
URI: http://hdl.handle.net/2445/104042
ISSN: 2158-3188
Appears in Collections:Articles publicats en revistes (Medicina)

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