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|Title:||Treatment with G-CSF reduces acute myeloid leukemia blast viability in the presence of bone marrow stroma|
|Author:||Nomdedeu i Fàbrega, Meritxell|
Lara Castillo, María Carmen
Cornet Masana, Josep M.
Díaz Beyá, Marina
Esteve Reyner, Jordi
Risueño, Ruth M.
Factors de creixement
|Abstract:||BACKGROUND: The resulting clinical impact of the combined use of G-CSF with chemotherapy as a chemosensitizing strategy for treatment of acute myeloid leukemia (AML) patients is still controversial. In this study, the effect of ex vivo treatment with G-CSF on AML primary blasts was studied. METHODS: Peripheral blood mononuclear cells from AML patients were treated with G-CSF at increasing doses, alone or in co-culture with HS-5 stromal cells. Cell viability and surface phenotype was determined by flow cytometry 72 h after treatment. For clonogenicity assays, AML primary samples were treated for 18 h with G-CSF at increasing concentrations and cultured in methyl-cellulose for 14 days. Colonies were counted based on cellularity and morphology criteria. RESULTS: The presence of G-CSF reduced the overall viability of AML cells co-cultured with bone marrow stroma; whereas, in absence of stroma, a negligible effect was observed. Moreover, clonogenic capacity of AML cells was significantly reduced upon treatment with G-CSF. Interestingly, reduction in the AML clonogenic capacity correlated with the sensitivity to chemotherapy observed in vivo. CONCLUSIONS: These ex vivo results would provide a biological basis to data available from studies showing a clinical benefit with the use of G-CSF as a priming agent in patients with a chemosensitive AML and would support implementation of further studies exploring new strategies of chemotherapy priming in AML.|
|Note:||Reproducció del document publicat a: https://doi.org/10.1186/s12935-015-0272-3|
|It is part of:||Cancer Cell International, 2015, vol. 15, p. 122|
|Appears in Collections:||Articles publicats en revistes (Medicina)|
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