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Title: Cyclic AMP signaling restricts activation and promotes maturation and antioxidant defenses in astrocytes
Author: Paco Mercader, Sonia
Hummel, Manuela
Plá Virginia
Sumoy, Lauro
Aguado Tomàs, Fernando
Keywords: Antidiabètics
Hypoglucemic agents
Issue Date: 23-Apr-2016
Publisher: BioMed Central
Abstract: Abstract Background: cAMP signaling produces dramatic changes in astrocyte morphology and physiology. However, its involvement in phenotype acquisition and the transcriptionally mediated mechanisms of action are largely unknown. Results: Here we analyzed the global transcriptome of cultured astroglial cells incubated with activators of cAMP pathways. A bulk of astroglial transcripts, 6221 annotated genes, were differentially regulated by cAMP signaling. cAMP analogs strongly upregulated genes involved in typical functions of mature astrocytes, such as homeostatic control, metabolic and structural support to neurons, antioxidant defense and communication, whereas they downregulated a considerable number of proliferating and immaturity-related transcripts. Moreover, numerous genes typically activated in reactive cells, such as scar components and immunological mediators, were repressed by cAMP. GSEA analysis contrasting gene expression profiles with transcriptome signatures of acutely isolated astrocytes and in situ evaluation of protein levels in these cells showed that cAMP signaling conferred mature and in vivo-like transcriptional features to cultured astrocytes. Conclusions: These results indicate that cAMP signaling is a key pathway promoting astrocyte maturation and restricting their developmental and activation features. Therefore, a positive modulation of cAMP signaling may promote the normal state of differentiated astrocytes and favor the protection and function of neuronal networks. Keywords: Antioxidant defense, Astrocytes, cAMP, Differentiation, Reactive glia, Transcriptomic, Brain, NR2C
Note: Reproducció del document publicat a:
It is part of: Bmc Genomics, 2016, vol. 17, num. 304, p. 1-11
Related resource:
ISSN: 1471-2164
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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