Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/104307
Title: Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
Author: Pérez-Cruz, Carla
Delgado, Lídia
López-Iglesias, Carmen
Mercadé Gil, M. Elena
Keywords: Membranes cel·lulars
Bacteris gramnegatius
Bacteris patògens
Cell membranes
Gram-negative bacteria
Pathogenic bacteria
Issue Date: 12-Jan-2015
Publisher: Public Library of Science (PLoS)
Abstract: Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles. To demonstrate that this is not a singular phenomenon in a bacterium occurring in an extreme environment, the identification of O-IMVs in pathogenic bacteria was undertaken. With this aim, a structural study by Transmission Electron Microscopy (TEM) and Cryo-transmission electron microscopy (Cryo-TEM) was carried out, confirming that O-IMVs are also secreted by Gram-negative pathogenic bacteria such as Neisseria gonorrhoeae, Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii AB41, in which they represent between 0.23% and 1.2% of total vesicles produced. DNA and ATP, which are components solely found in the cell cytoplasm, were identified within membrane vesicles of these strains. The presence of DNA inside the O-IMVs produced by N. gonorrhoeae was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. A proteomic analysis of N. gonorrhoeae-derived membrane vesicles identified proteins from the cytoplasm and plasma membrane. This confirmation of O-IMV extends the hitherto uniform definition of membrane vesicles in Gram-negative bacteria and explains the presence of components in membrane vesicles such as DNA, cytoplasmic and inner membrane proteins, as well as ATP, detected for the first time. The production of these O-IMVs by pathogenic Gram-negative bacteria opens up new areas of study related to their involvement in lateral gene transfer, the transfer of cytoplasmic proteins, as well as the functionality and role of ATP detected in these new vesicles
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0116896
It is part of: PLoS One, 2015, vol. 10, num. 1, p. e0116896
Related resource: https://doi.org/10.1371/journal.pone.0116896
URI: http://hdl.handle.net/2445/104307
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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