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Title: Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast
Author: Scheler, Ulschan
Brandt, Wolfgang
Porzel, Andrea
Rothe, Kathleen
Manzano Alías, David
Bozic, Dragana
Papaefthimiou, Dimitra
Balcke, Gerd Ulrich
Henning, Anja
Lohse, Swanhild
Marillonnet, Sylvestre
Kanellis, Angelos K.
Ferrer i Prats, Albert
Tissier, Alain
Keywords: Additius alimentaris
Llevats (Botànica)
Food additives
Yeast fungi
Issue Date: 5-Oct-2016
Publisher: Nature Publishing Group
Abstract: Rosemary extracts containing the phenolic diterpenes carnosic acid and its derivative carnosol are approved food additives used in an increasingly wide range of products to enhance shelf-life, thanks to their high anti-oxidant activity. We describe here the elucidation of the complete biosynthetic pathway of carnosic acid and its reconstitution in yeast cells. Cytochrome P450 oxygenases (CYP76AH22-24) from Rosmarinus officinalis and Salvia fruticosa already characterized as ferruginol synthases are also able to produce 11-hydroxyferruginol. Modelling-based mutagenesis of three amino acids in the related ferruginol synthase (CYP76AH1) from S. miltiorrhiza is sufficient to convert it to a 11-hydroxyferruginol synthase (HFS). The three sequential C20 oxidations for the conversion of 11-hydroxyferruginol to carnosic acid are catalysed by the related CYP76AK6-8. The availability of the genes for the biosynthesis of carnosic acid opens opportunities for the metabolic engineering of phenolic diterpenes, a class of compounds with potent anti-oxidant, anti-inflammatory and anti-tumour activities.
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It is part of: Nature Communications, 2016, vol. 7, p. 1-11
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ISSN: 2041-1723
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)

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