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http://hdl.handle.net/2445/104424
Title: | Maslinic acid, a natural Triterpene, induces a death receptor-mediated apoptotic mechanism in Caco-2 p53-deficient colon adenocarcinoma cells |
Author: | Reyes-Zurita, Fernando J. Rufino Palomares, Eva E. García Salguero, Leticia Peragón, Juan Medina, Pedro P. Parra Puerto, Andrés Cascante i Serratosa, Marta Lupiáñez, José A. |
Keywords: | Olis vegetals Càncer colorectal Diferenciació cel·lular Proteïnes Vegetable oils Colorectal cancer Cell diferentiation Proteins |
Issue Date: | 11-Jan-2016 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mito- chondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dose- dependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases -8 and -3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondr ial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0146178 |
It is part of: | PLoS One, 2016, vol. 11, num. 1, p. e0146178 |
URI: | http://hdl.handle.net/2445/104424 |
Related resource: | https://doi.org/10.1371/journal.pone.0146178 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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