Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/104523
Title: HepatoDyn: a dynamic model of hepatocyte metabolism that integrates 13C isotopomer data
Author: Foguet, Carles
Marín Martínez, Silvia
Selivanov, Vitaly A.
Fanchon, Eric
Lee, Paul W. N
Guinovart, Joan J. (Joan Josep), 1947-
Atauri Carulla, Ramón de
Cascante i Serratosa, Marta
Keywords: Hepatologia
Isòtops
Metabolisme
Hepatology
Isotopes
Metabolism
Issue Date: 28-Apr-2016
Publisher: Public Library of Science (PLoS)
Abstract: The liver performs many essential metabolic functions, which can be studied using computational models of hepatocytes. Here we present HepatoDyn, a highly detailed dynamic model of hepatocyte metabolism. HepatoDyn includes a large metabolic network, highly detailed kinetic laws, and is capable of dynamically simulating the redox and energy metabolism of hepatocytes. Furthermore, the model was coupled to the module for isotopic label propagation of the software package IsoDyn, allowing HepatoDyn to integrate data derived from 13C based experiments. As an example of dynamical simulations applied to hepatocytes, we studied the effects of high fructose concentrations on hepatocyte metabolism by integrating data from experiments in which rat hepatocytes were incubated with 20 mM glucose supplemented with either 3 mM or 20 mM fructose. These experiments showed that glycogen accumulation was significantly lower in hepatocytes incubated with medium supplemented with 20 mM fructose than in hepatocytes incubated with medium supplemented with 3 mM fructose. Through the integration of extracellular fluxes and 13C enrichment measurements, HepatoDyn predicted that this phenomenon can be attributed to a depletion of cytosolic ATP and phosphate induced by high fructose concentrations in the medium.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pcbi.1004899
It is part of: PLoS Computational Biology, 2016, vol. 12, num. 4, p. e1004899
Related resource: https://doi.org/10.1371/journal.pcbi.1004899
URI: http://hdl.handle.net/2445/104523
ISSN: 1553-734X
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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