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Title: Blockade of the SNARE protein syntaxin 1 inhibits glioblastoma tumor growth
Author: Ulloa Darquea, Fausto Alexander
Gonzàlez Juncà, Alba
Meffre, Delphine
Barrecheguren Manero, Pablo José
Martínez Mármol, Ramón
Pazos, Irene
Olivé, Núria
Cotrufo, Tiziana
Seoane Suárez, Joan
Soriano García, Eduardo
Keywords: Proteïnes citosquelètiques
Cytoskeletal proteins
Issue Date: 24-Mar-2015
Publisher: Public Library of Science (PLoS)
Abstract: Glioblastoma (GBM) is the most prevalent adult brain tumor, with virtually no cure, and with a median overall survival of 15 months from diagnosis despite of the treatment. SNARE pro- teins mediate membrane fusion events in cells and are essential for many cellular process- es including exocytosis and neurotransmission, intracellular trafficking and cell migration. Here we show that the blockade of the SNARE protein Syntaxin 1 (Stx1) function impairs GBM cell proliferation. We show that Stx1 loss-of-function in GBM cells, through ShRNA lentiviral transduction, a Stx1 dominant negative and botulinum toxins, dramatically reduces the growth of GBM after grafting U373 cells into the brain of immune compromised mice. In- terestingly, Stx1 role on GBM progression may not be restricted just to cell proliferation since the blockade of Stx1 also reduces in vitro GBM cell invasiveness suggesting a role in several processes relevant for tumor progression. Altogether, our findings indicate that the blockade of SNARE proteins may represent a novel therapeutic tool against GBM.
Note: Reproducció del document publicat a:
It is part of: PLoS One, 2015, vol. 10, num. 3, p. e0119707
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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