Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/104548
Title: The disruption of GDP-fucose de novo biosynthesis suggests the presence of a novel fucose-containing glycoconjugate in Plasmodium asexual blood stages
Author: Sanz, Sílvia
López Gutiérrez, Borja
Bandini, Giulia
Damerow, Sebastian
Absalon, Sabrina
Dinglasan, Rhoel R.
Samuelson, John
Izquierdo, Luis
Keywords: Malària
Malalties parasitàries
Malaria
Parasitic diseases
Issue Date: 16-Nov-2016
Publisher: Springer Nature
Abstract: Glycosylation is an important posttranslational protein modification in all eukaryotes. Besides glycosylphosphatidylinositol (GPI) anchors and N-glycosylation, O-fucosylation has been recently reported in key sporozoite proteins of the malaria parasite. Previous analyses showed the presence of GDP-fucose (GDP-Fuc), the precursor for all fucosylation reactions, in the blood stages of Plasmodium falciparum. The GDP-Fuc de novo pathway, which requires the action of GDP-mannose 4,6-dehydratase (GMD) and GDP-L-fucose synthase (FS), is conserved in the parasite genome, but the importance of fucose metabolism for the parasite is unknown. To functionally characterize the pathway we generated a PfGMD mutant and analyzed its phenotype. Although the labelling by the fucose-binding Ulex europaeus agglutinin I (UEA-I) was completely abrogated, GDP-Fuc was still detected in the mutant. This unexpected result suggests the presence of an alternative mechanism for maintaining GDP-Fuc in the parasite. Furthermore, PfGMD null mutant exhibited normal growth and invasion rates, revealing that the GDP-Fuc de novo metabolic pathway is not essential for the development in culture of the malaria parasite during the asexual blood stages. Nonetheless, the function of this metabolic route and the GDP-Fuc pool that is generated during this stage may be important for gametocytogenesis and sporogonic development in the mosquito.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1038/srep37230
It is part of: Scientific Reports, 2016, vol. 6, num. 37230
Related resource: http://dx.doi.org/10.1038/srep37230
URI: http://hdl.handle.net/2445/104548
ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (ISGlobal)

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