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Title: Loss of p27/Kip1 promotes metaplasia in the pancreas via the regulation of Sox9 expression.
Author: Jeannot, Pauline
Callot, Caroline
Baer, Romain
Duquesnes, Nicolas
Guerra, Carmen
Guillermet-Guibert, Julie
Bachs Valldeneu, Oriol
Besson, Arnaud
Keywords: Pàncrees
Cicle cel·lular
Inhibidors enzimàtics
Proteïnes quinases
Cell cycle
Enzyme inhibitors
Protein kinases
Issue Date: 3-Nov-2015
Publisher: Impact Journals
Abstract: p27Kip1 (p27) is a negative regulator of proliferation and a tumor suppressor via the inhibition of cyclin-CDK activity in the nucleus. p27 is also involved in the regulation of other cellular processes, including transcription by acting as a transcriptional co-repressor. Loss of p27 expression is frequently observed in pancreatic adenocarcinomas in human and is associated with decreased patient survival. Similarly, in a mouse model of K-Ras-driven pancreatic cancer, loss of p27 accelerates tumor development and shortens survival, suggesting an important role for p27 in pancreatic tumorigenesis. Here, we sought to determine how p27 might contribute to early events leading to tumor development in the pancreas. We found that K-Ras activation in the pancreas causes p27 mislocalization at pre-neoplastic stages. Moreover, loss of p27 or expression of a mutant p27 that does not bind cyclin-CDKs causes the mislocalization of several acinar polarity markers associated with metaplasia and induces the nuclear expression of Sox9 and Pdx1 two transcription factors involved in acinar-to-ductal metaplasia. Finally, we found that p27 directly represses transcription of Sox9, but not that of Pdx1. Thus, our results suggest that K-Ras activation, the earliest known event in pancreatic carcinogenesis, may cause loss of nuclear p27 expression which results in derepression of Sox9, triggering reprogramming of acinar cells and metaplasia.
Note: Reproducció del document publicat a:
It is part of: Oncotarget, 2015, vol. 6, num. 34, p. 35880-35892
Related resource:
ISSN: 1949-2553
Appears in Collections:Articles publicats en revistes (Biomedicina)

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