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Title: Cyclin A1 is essential for setting the pluripotent state and reducing tumorigenicity of induced pluripotent stem cells
Author: McLenachan, Samuel
Menchón Najas, Cristina
Raya Chamorro, Ángel
Consiglio, Antonella
Edel, Michael John
Keywords: Cèl·lules mare
Cèl·lules mare embrionàries
Cicle cel·lular
Transformació cel·lular
Proteïnes supressores de tumors
Stem cells
Embryonic stem cells
Cell cycle
Cell transformation
Tumor suppressor protein
Issue Date: Apr-2012
Publisher: Mary Ann Liebert
Abstract: The proper differentiation and threat of cancer rising from the application of induced pluripotent stem (iPS) cells are major bottlenecks in the field and are thought to be inherently linked to the pluripotent nature of iPS cells. To address this question, we have compared iPS cells to embryonic stem cells (ESCs), the gold standard of ground state pluripotency, in search for proteins that may improve pluripotency of iPS cells. We have found that when reprogramming somatic cells toward pluripotency, 1%-5% of proteins of 5 important cell functions are not set to the correct expression levels compared to ESCs, including mainly cell cycle proteins. We have shown that resetting cyclin A1 protein expression of early-passage iPS cells closer to the ground state pluripotent state of mouse ESCs improves the pluripotency and reduces the threat of cancer of iPS cells. This work is a proof of principle that reveals that setting expression of certain proteins correctly during reprogramming is essential for achieving ESC-state pluripotency. This finding would be of immediate help to those researchers in different fields of iPS cell work that specializes in cell cycle, apoptosis, cell adhesion, cell signaling, and cytoskeleton.
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It is part of: Stem Cells and Development, 2012, vol. 21, num. 15, p. 2891-2899
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ISSN: 1547-3287
Appears in Collections:Articles publicats en revistes (Institut de Biomedicina (IBUB))
Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

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