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http://hdl.handle.net/2445/107063
Title: | Actin filaments are involved in the functional coupling of Vo-V1 domains of vacuolar H-ATPase at the Golgi complex |
Author: | Serra-Peinado, Carla Sicart Casellas, Adrià Llopis, Juan Egea Guri, Gustavo |
Keywords: | Aparell de Golgi Citosquelet Proteïnes citosquelètiques Enzimologia Homeòstasi Golgi apparatus Cytoskeleton Cytoskeletal proteins Enzymology Homeostasis |
Issue Date: | 12-Feb-2016 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | We previously reported that actin-depolymerizing agents promote the alkalization of the Golgi stack and thetrans-Golgi network. The main determinant of acidic pH at the Golgi is the vacuolar-type H(+)-translocating ATPase (V-ATPase), whose V1domain subunitsBandCbind actin. We have generated a GFP-tagged subunitB2construct (GFP-B2) that is incorporated into the V1domain, which in turn is coupled to the V0sector. GFP-B2 subunit is enriched at distal Golgi compartments in HeLa cells. Subcellular fractionation, immunoprecipitation, and inversal FRAP experiments show that the actin depolymerization promotes the dissociation of V1-V0domains, which entails subunitB2translocation from Golgi membranes to the cytosol. Moreover, molecular interaction between subunitsB2andC1and actin were detected. In addition, Golgi membrane lipid order disruption byd-ceramide-C6 causes Golgi pH alkalization. We conclude that actin regulates the Golgi pH homeostasis maintaining the coupling of V1-V0domains of V-ATPase through the binding of microfilaments to subunitsBandCand preserving the integrity of detergent-resistant membrane organization. These results establish the Golgi-associated V-ATPase activity as the molecular link between actin and the Golgi pH. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M115.675272 |
It is part of: | Journal of Biological Chemistry, 2016, vol. 291, num. 14, p. 7286-7299 |
URI: | http://hdl.handle.net/2445/107063 |
Related resource: | https://doi.org/10.1074/jbc.M115.675272 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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