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http://hdl.handle.net/2445/107125
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DC Field | Value | Language |
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dc.contributor.author | Marimon Garrido, Oriol | - |
dc.contributor.author | Teixeira, Joao M. C. | - |
dc.contributor.author | Cordeiro, Tiago N. | - |
dc.contributor.author | Soo, Valerie W. C. | - |
dc.contributor.author | Wood, Thammajun L. | - |
dc.contributor.author | Mayzel, Maxim | - |
dc.contributor.author | Amata, Irene | - |
dc.contributor.author | Gracía, Jesús | - |
dc.contributor.author | Morera, Ainara | - |
dc.contributor.author | Gay i Marín, Marina | - |
dc.contributor.author | Vilaseca Casas, Marta | - |
dc.contributor.author | Orekhov, Vladislav Yu | - |
dc.contributor.author | Wood, Thomas K. | - |
dc.contributor.author | Pons Vallès, Miquel | - |
dc.date.accessioned | 2017-02-20T11:20:56Z | - |
dc.date.available | 2017-02-20T11:20:56Z | - |
dc.date.issued | 2016-12-08 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/2445/107125 | - |
dc.description.abstract | The Hha and TomB proteins from Escherichia coli form an oxygen-dependent toxin-antitoxin (TA) system. Here we show that YmoB, the Yersinia orthologue of TomB, and its single cysteine variant [C117S]YmoB can replace TomB as antitoxins in E. coli. In contrast to other TA systems, [C117S]YmoB transiently interacts with Hha (rather than forming a stable complex) and enhances the spontaneous oxidation of the Hha conserved cysteine residue to a -SOxH- containing species (sulfenic, sulfinic or sulfonic acid), which destabilizes the toxin. The nuclear magnetic resonance structure of [C117S]YmoB and the homology model of TomB show that the two proteins form a four-helix bundle with a conserved buried cysteine connected to the exterior by a channel with a diameter comparable to that of an oxygen molecule. The Hha interaction site is located on the opposite side of the helix bundle. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/ncomms13634 | - |
dc.relation.ispartof | Nature Communications, 2016, vol. 7, num. 13634 | - |
dc.relation.uri | https://doi.org/10.1038/ncomms13634 | - |
dc.rights | cc-by (c) Marimon Garrido, Oriol et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Química Inorgànica i Orgànica) | - |
dc.subject.classification | Espectrometria de masses | - |
dc.subject.classification | Espectroscòpia de ressonància magnètica nuclear | - |
dc.subject.classification | Escheríchia coli | - |
dc.subject.other | Mass spectrometry | - |
dc.subject.other | Nuclear magnetic resonance spectroscopy | - |
dc.subject.other | Escherichia coli | - |
dc.title | An oxygen-sensitive toxin-antitoxin system | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 667829 | - |
dc.date.updated | 2017-02-20T11:20:56Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/261863/EU//BIO-NMR | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27929062 | - |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
File | Description | Size | Format | |
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667829.pdf | 1.94 MB | Adobe PDF | View/Open |
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