Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/108258
Title: Structure of the Homodimeric androgen receptor ligand-binding domain
Author: Nadal, Marta
Prekovic, Stefan
Gallastegui, Nerea
Helsen, Christine
Abella, Montserrat
Zielinska, Karolina
Gay, Marina
Vilaseca, Marta
Taulés i Marín, Marta
Houtsmuller, Adriaan B.
van Royen, Martin E.
Claessens, Frank
Fuentes-Prior, Pablo
Estébanez Perpiñá, Eva
Keywords: Receptors d'hormones
Difracció de raigs X
Radiocristal·lografia
Hormone receptors
X-rays diffraction
X-ray crystallography
Issue Date: 6-Feb-2017
Publisher: Nature Publishing Group
Abstract: The androgen receptor (AR) plays a crucial role in normal physiology, development and metabolism as well as in the aetiology and treatment of diverse pathologies such as androgen insensitivity syndromes (AIS), male infertility and prostate cancer (PCa). Here we show that dimerization of AR ligand-binding domain (LBD) is induced by receptor agonists but not by antagonists. The 2.15-Å crystal structure of homodimeric, agonist- and coactivator peptide-bound AR-LBD unveils a 1,000-Å2 large dimerization surface, which harbours over 40 previously unexplained AIS- and PCa-associated point mutations. An AIS mutation in the self-association interface (P767A) disrupts dimer formation in vivo, and has a detrimental effect on the transactivating properties of full-length AR, despite retained hormone-binding capacity. The conservation of essential residues suggests that the unveiled dimerization mechanism might be shared by other nuclear receptors. Our work defines AR-LBD homodimerization as an essential step in the proper functioning of this important transcription factor.
Note: Reproducció del document publicat a: https://doi.org/10.1038/ncomms14388
It is part of: Nature Communications, 2017, vol. 8, p. 14388-14398
Related resource: https://doi.org/10.1038/ncomms14388
URI: http://hdl.handle.net/2445/108258
ISSN: 2041-1723
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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