Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/108555
Title: An intriguing shift occurs in the novel protein phosphatase 1 binding partner, TCTEX1D4: evidence of positive selection in a pika model
Author: Korrodi-Gregório, Luís
Lopes, Ana Margarida
Esteves, Sara L. C.
Afonso, Sandra
Lemos de Matos, Ana
Lissovsky, Andrey A.
Cruz e Silva, Odete A. B. da
Cruz e Silva, Edgar F. da
Esteves, Pedro José
Fardilha, Margarida
Keywords: Mutagènesi
Selecció natural
Lagomorfs
Fosfatases
Mutagenesis
Natural selection
Lagomorpha
Phosphatases
Issue Date: 10-Oct-2013
Publisher: Public Library of Science (PLoS)
Abstract: T-complex testis expressed protein 1 domain containing 4 (TCTEX1D4) contains the canonical phosphoprotein phosphatase 1 (PPP1) binding motif, composed by the amino acid sequence RVSF. We identified and validated the binding of TCTEX1D4 to PPP1 and demonstrated that indeed this protein is a novel PPP1 interacting protein. Analyses of twenty-one mammalian species available in public databases and seven Lagomorpha sequences obtained in this work showed that the PPP1 binding motif 90RVSF93 is present in all of them and is flanked by a palindromic sequence, PLGS, except in three species of pikas (Ochotona princeps, O. dauurica and O. pusilla). Furthermore, for the Ochotona species an extra glycosylation site, motif 96NLS98, and the loss of the palindromic sequence were observed. Comparison with other lagomorphs suggests that this event happened before the Ochotona radiation. The dN/dS for the sequence region comprising the PPP1 binding motif and the flanking palindrome highly supports the hypothesis that for Ochotona species this region has been evolving under positive selection. In addition, mutational screening shows that the ability of pikas TCTEX1D4 to bind to PPP1 is maintained, although the PPP1 binding motif is disrupted, and the N- and C-terminal surrounding residues are also abrogated. These observations suggest pika as an ideal model to study novel PPP1 complexes regulatory mechanisms.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.007723
It is part of: PLoS One, 2013, vol. 8, num. 10, p. e77236
Related resource: https://doi.org/10.1371/journal.pone.007723
URI: http://hdl.handle.net/2445/108555
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

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