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http://hdl.handle.net/2445/108570
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DC Field | Value | Language |
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dc.contributor.author | Rúbies Prat, Antoni | - |
dc.contributor.author | Centrich, Francesc | - |
dc.contributor.author | Granados i Juan, Mercè | - |
dc.date.accessioned | 2017-03-17T14:30:49Z | - |
dc.date.available | 2017-06-20T22:01:23Z | - |
dc.date.issued | 2016-06-20 | - |
dc.identifier.issn | 1618-2642 | - |
dc.identifier.uri | http://hdl.handle.net/2445/108570 | - |
dc.description.abstract | We developed a Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method for the high throughput determination of 10 non-steroidal anti-inflammatory drugs (NSAIDs) in milk samples using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with a triple quadrupole (QqQ) instrument and an electrospray ionization (ESI) source. The new extraction procedure is highly efficient, and we obtained absolute recoveries in the range 78.1-97.1 % for the extraction and clean-up steps. Chromatographic separation is performed in the gradient mode with a biphenyl column and acidic mobile phases consisting of water and acetonitrile containing formic acid. The chromatographic run time was about 12 min, and NSAID peaks showed a good symmetry factor. For MS/MS detection, we used multiple reaction monitoring (MRM) mode, using ESI in both positive and negative modes. Our method has been validated in compliance with the European Commission Decision 657/2002/EC, and we obtained very satisfactory results in inter-laboratory testing. Furthermore, we explored the use of a hybrid high resolution mass spectrometer, combining a quadrupole and an Orbitrap mass analyzer, for high resolution (HR) MS/MS detection of NSAIDs. We achieved lower NSAID quantification limits with Q-Orbitrap high resolution mass spectrometry (HRMS/MS) detection than those achieved with the QqQ instrument; however, its main feature is its very high selectivity, which makes HRMS/MS particularly suitable for confirmatory analysis. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Verlag | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1007/s00216-016-9679-5 | - |
dc.relation.ispartof | Analytical and Bioanalytical Chemistry, 2016, vol. 408, num. 21, p. 5769-5778 | - |
dc.relation.uri | https://doi.org/10.1007/s00216-016-9679-5 | - |
dc.rights | (c) Springer Verlag, 2016 | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | Cromatografia de líquids | - |
dc.subject.classification | Espectrometria de masses | - |
dc.subject.classification | Antiinflamatoris no esteroïdals | - |
dc.subject.classification | Llet | - |
dc.subject.other | Liquid chromatography | - |
dc.subject.other | Mass spectrometry | - |
dc.subject.other | Nonsteroidal anti-inflammatory agents | - |
dc.subject.other | Milk | - |
dc.title | Analysis of non-steroidal anti-inflammatory drugs in milk using QuEChERS and liquid chromatography coupled to mass spectrometry: triple quadrupole versus Q-Orbitrap mass analyzers | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 669932 | - |
dc.date.updated | 2017-03-17T14:30:50Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27325465 | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
Files in This Item:
File | Description | Size | Format | |
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669932.pdf | 149.34 kB | Adobe PDF | View/Open |
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