Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/108889
Title: Epigenomic analysis detects aberrant super-enhancer DNA methylation in human cancer
Author: Heyn, Holger
Vidal, Enrique
Ferreira, Humberto J.
Vizoso, Miguel
Sayols, Sergi
Gómez, Antonio
Moran, Sebastian
Boque-Sastre, Raquel
Guil, Sonia
Martinez-Cardús, Anna
Lin, Charles Y.
Royo, Romina
Sanchez-Mut, Jose Vicente
Martinez, Ramon
Gut, Marta
Torrents, David
Orozco López, Modesto
Gut, Ivo
Young, Richard A.
Esteller, Manel
Keywords: ADN
Epigènesi
Genètica
Metilació
Càncer
DNA
Epigenesis
Genetics
Methylation
Cancer
Issue Date: 26-Jan-2016
Publisher: BioMed Central
Abstract: Background: One of the hallmarks of cancer is the disruption of gene expression patterns. Many molecular lesions contribute to this phenotype, and the importance of aberrant DNA methylation profiles is increasingly recognized. Much of the research effort in this area has examined proximal promoter regions and epigenetic alterations at other loci are not well characterized. Results: Using whole genome bisulfite sequencing to examine uncharted regions of the epigenome, we identify a type of far-reaching DNA methylation alteration in cancer cells of the distal regulatory sequences described as super-enhancers. Human tumors undergo a shift in super-enhancer DNA methylation profiles that is associated with the transcriptional silencing or the overactivation of the corresponding target genes. Intriguingly, we observe locally active fractions of super-enhancers detectable through hypomethylated regions that suggest spatial variability within the large enhancer clusters. Functionally, the DNA methylomes obtained suggest that transcription factors contribute to this local activity of super-enhancers and that trans-acting factors modulate DNA methylation profiles with impact on transforming processes during carcinogenesis. Conclusions: We develop an extensive catalogue of human DNA methylomes at base resolution to better understand the regulatory functions of DNA methylation beyond those of proximal promoter gene regions. CpG methylation status in normal cells points to locally active regulatory sites at super-enhancers, which are targeted by specific aberrant DNA methylation events in cancer, with putative effects on the expression of downstream genes.
Note: Reproducció del document publicat a: https://doi.org/10.1186/s13059-016-0879-2
It is part of: Genome Biology, 2016, vol. 17, p. 11
Related resource: https://doi.org/10.1186/s13059-016-0879-2
URI: http://hdl.handle.net/2445/108889
ISSN: 1474-7596
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Files in This Item:
File Description SizeFormat 
662704.pdf3.76 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons