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http://hdl.handle.net/2445/108942
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DC Field | Value | Language |
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dc.contributor.author | Sindreu Balet, Carlos | - |
dc.contributor.author | Bayés, Àlex | - |
dc.contributor.author | Altafaj, Xavier | - |
dc.contributor.author | Pérez Clausell, Jeús | - |
dc.date.accessioned | 2017-03-27T10:04:05Z | - |
dc.date.available | 2017-03-27T10:04:05Z | - |
dc.date.issued | 2014-03-07 | - |
dc.identifier.issn | 1756-6606 | - |
dc.identifier.uri | http://hdl.handle.net/2445/108942 | - |
dc.description.abstract | BACKGROUND: Zinc concentrates at excitatory synapses, both at the postsynaptic density and in a subset of glutamatergic boutons. Zinc can modulate synaptic plasticity, memory formation and nociception by regulating transmitter receptors and signal transduction pathways. Also, intracellular zinc accumulation is a hallmark of degenerating neurons in several neurological disorders. To date, no single zinc extrusion mechanism has been directly localized to synapses. Based on the presence of a canonical PDZ I motif in the Zinc Transporter-1 protein (ZnT1), we hypothesized that ZnT1 may be targeted to synaptic compartments for local control of cytosolic zinc. Using our previously developed protocol for the co-localization of reactive zinc and synaptic proteins, we further asked if ZnT1 expression correlates with presynaptic zinc content in individual synapses. FINDINGS: Here we demonstrate that ZnT1 is a plasma membrane protein that is enriched in dendritic spines and in biochemically isolated synaptic membranes. Hippocampal CA1 synapses labelled by postembedding immunogold showed over a 5-fold increase in ZnT1 concentration at synaptic junctions compared with extrasynaptic membranes. Subsynaptic analysis revealed a peak ZnT1 density on the postsynaptic side of the synapse, < 10 nm away from the postsynaptic membrane. ZnT1 was found in the vast majority of excitatory synapses regardless of the presence of vesicular zinc in presynaptic boutons. CONCLUSIONS: Our study has identified ZnT1 as a novel postsynaptic density protein, and it may help elucidate the role of zinc homeostasis in synaptic function and disease. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1186/1756-6606-7-16 | - |
dc.relation.ispartof | Molecular Brain, 2014, vol. 7, num. 1, p. 16-22 | - |
dc.relation.uri | https://doi.org/10.1186/1756-6606-7-16 | - |
dc.rights | cc-by (c) Sindreu Balet, Carlos et al., 2014 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Zinc | - |
dc.subject.classification | Sinapsi | - |
dc.subject.classification | Neurotransmissors | - |
dc.subject.classification | Rates (Animals de laboratori) | - |
dc.subject.other | Zinc | - |
dc.subject.other | Synapses | - |
dc.subject.other | Neurotransmitters | - |
dc.subject.other | Rats as laboratory animals | - |
dc.title | Zinc Transporter-1 Concentrates at the Postsynaptic Density of Hippocampal Synapses | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 633729 | - |
dc.date.updated | 2017-03-27T10:04:05Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 24602382 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Medicina) Articles publicats en revistes (Fonaments Clínics) |
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File | Description | Size | Format | |
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633729.pdf | 1.79 MB | Adobe PDF | View/Open |
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