Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/109007
Title: Sugar nucleotide quantification by liquid chromatography tandem mass spectrometry reveals a distinct profile in Plasmodium falciparum sexual stage parasites
Author: López Gutiérrez, Borja
Dinglasan, Rhoel R.
Izquierdo, Luis
Keywords: Nucleòtids
Plasmodium falciparum
Nucleotides
Plasmodium falciparum
Issue Date: 7-Mar-2017
Publisher: Biochemical Society
Abstract: The obligate intracellular lifestyle of Plasmodium falciparum and the difficulties to obtain sufficient amounts of biological material has hampered the study of specific metabolic pathways in the malaria parasite. Thus, for example, the pools of sugar nucleotides required to fuel glycosylation reactions have never been studied in-depth in well-synchronized asexual parasites or in other stages of its life cycle. These metabolites are of critical importance, especially considering the renewed interest in the presence of N -, O - and other glycans in key parasite proteins. In this work, we adapted a liquid chromatography tandem mass spectrometry method based on the use of porous graphitic carbon (PGC) columns and MS-friendly solvents to quantify sugar nucleotides in the malaria parasite. We report on the thorough quantification of the pools of these metabolites throughout the intraerythrocytic cycle of P. falciparum The sensitivity of the method enabled for the first time the targeted analysis of these glycosylation precursors in gametocytes, the parasite sexual stages that are transmissible to the mosquito vector.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1042/BCJ20161030
It is part of: The Biochemical Journal, 2017, vol. 6, num. 474, p. 897-905
Related resource: http://dx.doi.org/10.1042/BCJ20161030
URI: http://hdl.handle.net/2445/109007
ISSN: 0264-6021
Appears in Collections:Articles publicats en revistes (ISGlobal)

Files in This Item:
File Description SizeFormat 
sugar nucleotide.pdf505.24 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.