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Title: α-Actinin links extracellular matrix rigidity-sensing contractile units with periodic cell-edge retractions
Author: Meacci, Giovanni
Wolfenson, Haguy
Liu, Shuaimin
Stachowiak, Matthew R.
Iskratsch, Thomas
Mathur, Anurag
Ghassemi, Saba
Gauthier, Nils C.
Tabdanov, Erdem
Lohner, James
Gondarenko, Alexander
Chander, Ashok C.
Roca-Cusachs Soulere, Pere
O'shaughnessy, Ben
Hone, James
Sheetz, Michael P.
Keywords: Cèl·lules
Proteïnes citosquelètiques
Cytoskeletal proteins
Issue Date: 7-Nov-2016
Publisher: American Society for Cell Biology
Abstract: During spreading and migration, the leading edges of cells undergo periodic protrusion-retraction cycles. The functional purpose of these cycles is unclear. Here, using submicrometer polydimethylsiloxane pillars as substrates for cell spreading, we show that periodic edge retractions coincide with peak forces produced by local contractile units (CUs) that assemble and disassemble along the cell edge to test matrix rigidity. We find that, whereas actin rearward flow produces a relatively constant force inward, the peak of local contractile forces by CUs scales with rigidity. The cytoskeletal protein α-actinin is shared between these two force-producing systems. It initially localizes to the CUs and subsequently moves inward with the actin flow. Knockdown of α-actinin causes aberrant rigidity sensing, loss of CUs, loss of protrusion-retraction cycles, and, surprisingly, enables the cells to proliferate on soft matrices. We present a model based on these results in which local CUs drive rigidity sensing and adhesion formation.
Note: Reproducció del document publicat a:
It is part of: Molecular Biology of the Cell, 2016, vol. 27, num. 22, p. 3471-3479
Related resource:
ISSN: 1059-1524
Appears in Collections:Articles publicats en revistes (Biomedicina)

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