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http://hdl.handle.net/2445/109187
Title: | α-Actinin links extracellular matrix rigidity-sensing contractile units with periodic cell-edge retractions |
Author: | Meacci, Giovanni Wolfenson, Haguy Liu, Shuaimin Stachowiak, Matthew R. Iskratsch, Thomas Mathur, Anurag Ghassemi, Saba Gauthier, Nils C. Tabdanov, Erdem Lohner, James Gondarenko, Alexander Chander, Ashok C. Roca-Cusachs Soulere, Pere O'Shaughnessy, Ben Hone, James Sheetz, Michael P. |
Keywords: | Cèl·lules Proteïnes citosquelètiques Citosquelet Cells Cytoskeletal proteins Cytoskeleton |
Issue Date: | 7-Nov-2016 |
Publisher: | American Society for Cell Biology |
Abstract: | During spreading and migration, the leading edges of cells undergo periodic protrusion-retraction cycles. The functional purpose of these cycles is unclear. Here, using submicrometer polydimethylsiloxane pillars as substrates for cell spreading, we show that periodic edge retractions coincide with peak forces produced by local contractile units (CUs) that assemble and disassemble along the cell edge to test matrix rigidity. We find that, whereas actin rearward flow produces a relatively constant force inward, the peak of local contractile forces by CUs scales with rigidity. The cytoskeletal protein α-actinin is shared between these two force-producing systems. It initially localizes to the CUs and subsequently moves inward with the actin flow. Knockdown of α-actinin causes aberrant rigidity sensing, loss of CUs, loss of protrusion-retraction cycles, and, surprisingly, enables the cells to proliferate on soft matrices. We present a model based on these results in which local CUs drive rigidity sensing and adhesion formation. |
Note: | Reproducció del document publicat a: https://doi.org/10.1091/mbc.E16-02-0107 |
It is part of: | Molecular Biology of the Cell, 2016, vol. 27, num. 22, p. 3471-3479 |
URI: | http://hdl.handle.net/2445/109187 |
Related resource: | https://doi.org/10.1091/mbc.E16-02-0107 |
ISSN: | 1059-1524 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Publicacions de projectes de recerca finançats per la UE |
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