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Title: Structural characterization of core Region in Erwinia amylovora lipopolysaccharide.
Author: Casillo, Angela
Ziaco, Marcello
Lindner, Buko
Merino Montero, Susana
Mendoza-Barberá, Elena de
Tomás, Juan M.
Corsaro, Maria Michela
Keywords: Bacteris patògens
Genètica bacteriana
Pathogenic bacteria
Bacterial genetics
Issue Date: 4-Mar-2017
Publisher: MDPI
Abstract: Erwinia amylovora (E. amylovora) is the first bacterial plant pathogen described and demonstrated to cause fire blight, a devastating plant disease affecting a wide range of species including a wide variety of Rosaceae. In this study, we reported the lipopolysaccharide (LPS) core structure from E. amylovora strain CFBP1430, the first one for an E. amylovora highly pathogenic strain. The chemical characterization was performed on the mutants waaL (lacking only the O-antigen LPS with a complete LPS-core), wabH and wabG (outer-LPS core mutants). The LPSs were isolated from dry cells and analyzed by means of chemical and spectroscopic methods. In particular, they were subjected to a mild acid hydrolysis and/or a hydrazinolysis and investigated in detail by one and two dimensional Nuclear Magnetic Resonance (NMR) spectroscopy and ElectroSpray Ionization Fourier Transform-Ion Cyclotron Resonance (ESI FT-ICR) mass spectrometry.
Note: Reproducció del document publicat a:
It is part of: International Journal of Molecular Sciences, 2017, vol. 18, num. 3, p. 559-559
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ISSN: 1422-0067
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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