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Title: Gre factors-mediated control of hilD transcription is essential for the invasion of epithelial cells by Salmonella enterica serovar Typhimurium
Author: Gaviria-Cantin, Tania
El Mouali, Yossef
Le Guyon, Soazig
Römling, Ute
Balsalobre Parra, Carlos
Keywords: Salmonel·la
Transcripció genètica
Genetic transcription
Issue Date: 20-Apr-2017
Publisher: Public Library of Science (PLoS)
Abstract: The invasion of epithelial cells by Salmonella enterica serovar Typhimurium is a very tightly regulated process. Signaling cascades triggered by different environmental and physiological signals converge to control HilD, an AraC regulator that coordinates the expression of several virulence factors. The expression of hilD is modulated at several steps of the expression process. Here, we report that the invasion of epithelial cells by S. Typhimurium strains lacking the Gre factors, GreA and GreB, is impaired. By interacting with the RNA polymerase secondary channel, the Gre factors prevent backtracking of paused complexes to avoid arrest during transcriptional elongation. Our results indicate that the Gre factors are required for the expression of the bacterial factors needed for epithelial cell invasion by modulating expression of HilD. This regulation does not occur at transcription initiation and depends on the capacity of the Gre factors to prevent backtracking of the RNA polymerase. Remarkably, genetic analyses indicate that the 3'-untranslated region (UTR) of hilD is required for Gre-mediated regulation of hilD expression. Our data provide new insight into the complex regulation of S. Typhimurium virulence and highlight the role of the hilD 3'-UTR as a regulatory motif.
Note: Reproducció del document publicat a:
It is part of: PLoS Pathogens, 2017, vol. 13, num. 4, p. e1006312
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ISSN: 1553-7374
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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