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|Title:||Expression of the Plasmodium falciparum Clonally Variant clag3 Genes in Human Infections|
Schuppen, Evi van
Esbroeck, Marjan van
Rovira Graells, Núria
Gomez-Perez, Gloria P.
|Publisher:||Oxford University Press|
|Abstract:||Background.: Many genes of the malaria parasite Plasmodium falciparum show clonally variant expression regulated at the epigenetic level. These genes participate in fundamental host-parasite interactions and contribute to adaptive processes. However, little is known about their expression patterns during human infections. A peculiar case of clonally variant genes are the 2 nearly identical clag3 genes, clag3.1 and clag3.2, which mediate nutrient uptake and are linked to resistance to some toxic compounds. Methods.: We developed a procedure to characterize the expression of clag3 genes in naturally infected patients and in experimentally infected human volunteers. Results.: We provide the first description of clag3 expression during human infections, which revealed mutually exclusive expression and identified the gene predominantly expressed. Adaptation to culture conditions or selection with a toxic compound resulted in isolate-dependent changes in clag3 expression. We also found that clag3 expression patterns were reset during transmission stages. Conclusions.: Different environment conditions select for parasites with different clag3 expression patterns, implying functional differences between the proteins encoded. The epigenetic memory is likely erased before parasites start infection of a new human host. Altogether, our findings support the idea that clonally variant genes facilitate the adaptation of parasite populations to changing conditions through bet-hedging strategies.|
|Note:||Versió postprint del document publicat a: http://dx.doi.org/10.1093/infdis/jix053|
|It is part of:||The Journal of Infectious Diseases, 2017, vol. 215, num. 6 , p. 938-945|
|Appears in Collections:||Articles publicats en revistes (ISGlobal)|
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