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Title: 2-picolylamine derivatization for high sensitivity detection of abscisic acid in apicomplexan blood-infecting parasites
Author: Moles Meler, Ernest
Marcos, Josep
Imperial, Santiago
Pozo, Oscar J.
Fernàndez Busquets, Xavier
Keywords: Malària
Plasmodium falciparum
Protozous patògens
Àcid abscísic
Plasmodium falciparum
Pathogenic protozoa
Abscisic acid
Issue Date: 11-Mar-2017
Publisher: Elsevier Science Pub. Co.
Abstract: We have developed a new liquid chromatography-electrospray ionization tandem mass spectrometry methodology based on 2-picolylamine derivatization and positive ion mode detection for abscisic acid (ABA) identification. The selected reaction leads to the formation of an amide derivative which contains a highly active pyridyl group. The enhanced ionization allows for a 700-fold increase over commonly monitored unmodified ABA, which in turn leads to excellent limits of detection and quantification values of 0.03 and 0.15ngmL-1, respectively. This method has been validated in the highly complex matrix of a red blood cell extract. In spite of the high sensitivity achieved, ABA could not be detected in Plasmodium falciparum-infected red blood cells, suggesting that, if present, it will be found either in ultratrace amounts or as brief bursts at defined time points within the intraerythrocytic cycle and/or in the form of a biosynthetic analogue.
Note: Versió postprint del document publicat a:
It is part of: Talanta, 2017, vol. 168, p. 130-135
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ISSN: 0039-9140
Appears in Collections:Articles publicats en revistes (ISGlobal)

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