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http://hdl.handle.net/2445/110939
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DC Field | Value | Language |
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dc.contributor.author | Moles Meler, Ernest | - |
dc.contributor.author | Marcos, Josep | - |
dc.contributor.author | Imperial Ródenas, Santiago | - |
dc.contributor.author | Pozo, Oscar J. | - |
dc.contributor.author | Fernàndez Busquets, Xavier | - |
dc.date.accessioned | 2017-05-12T12:04:44Z | - |
dc.date.available | 2019-03-11T06:10:18Z | - |
dc.date.issued | 2017-03-11 | - |
dc.identifier.issn | 0039-9140 | - |
dc.identifier.uri | http://hdl.handle.net/2445/110939 | - |
dc.description.abstract | We have developed a new liquid chromatography-electrospray ionization tandem mass spectrometry methodology based on 2-picolylamine derivatization and positive ion mode detection for abscisic acid (ABA) identification. The selected reaction leads to the formation of an amide derivative which contains a highly active pyridyl group. The enhanced ionization allows for a 700-fold increase over commonly monitored unmodified ABA, which in turn leads to excellent limits of detection and quantification values of 0.03 and 0.15ngmL-1, respectively. This method has been validated in the highly complex matrix of a red blood cell extract. In spite of the high sensitivity achieved, ABA could not be detected in Plasmodium falciparum-infected red blood cells, suggesting that, if present, it will be found either in ultratrace amounts or as brief bursts at defined time points within the intraerythrocytic cycle and/or in the form of a biosynthetic analogue. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Science Pub. Co. | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.talanta.2017.03.030 | - |
dc.relation.ispartof | Talanta, 2017, vol. 168, p. 130-135 | - |
dc.relation.uri | http://dx.doi.org/10.1016/j.talanta.2017.03.030 | - |
dc.rights | cc by-nc-nd (c) Elsevier B.V., 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | - |
dc.source | Articles publicats en revistes (ISGlobal) | - |
dc.subject.classification | Malària | - |
dc.subject.classification | Plasmodium falciparum | - |
dc.subject.classification | Protozous patògens | - |
dc.subject.classification | Àcid abscísic | - |
dc.subject.other | Malaria | - |
dc.subject.other | Plasmodium falciparum | - |
dc.subject.other | Pathogenic protozoa | - |
dc.subject.other | Abscisic acid | - |
dc.title | 2-picolylamine derivatization for high sensitivity detection of abscisic acid in apicomplexan blood-infecting parasites | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.date.updated | 2017-05-03T18:01:32Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 28391832 | - |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
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File | Description | Size | Format | |
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moles2017_2502.pdf | 874.04 kB | Adobe PDF | View/Open |
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