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Title: MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma
Author: Sandoval, Juan
Díaz-Lagares, Angel
Salgado, Rocío
Servitje Bedate, Octavio
Climent, Fina
Ortiz Romero, P. L.
Pérez-Ferriols, Amparo
Garcia-Muret, Maria P.
Estrach Panella, Ma. Teresa (María Teresa)
Garcia, Mar
Nonell, Lara
Esteller, Manel
Pujol, Ramon M.
Espinet Solà, Blanca
Gallardo, Fernando
Keywords: Micro RNAs
Cèl·lules T
T cells
Issue Date: Apr-2015
Publisher: Society for Investigative Dermatology
Abstract: MicroRNAs usually regulate gene expression negatively, and aberrant expression has been involved in the development of several types of cancers. Microarray profiling of microRNA expression was performed to define a microRNA signature in a series of mycosis fungoides tumor stage (MFt, n=21) and CD30+ primary cutaneous anaplastic large cell lymphoma (CD30+ cALCL, n=11) samples in comparison with inflammatory dermatoses (ID, n=5). Supervised clustering confirmed a distinctive microRNA profile for cutaneous T-cell lymphoma (CTCL) with respect to ID. A 40 microRNA signature was found in MFt including upregulated onco-microRNAs (miR-146a, miR-142-3p/5p, miR-21, miR-181a/b, and miR-155) and downregulated tumor-suppressor microRNAs (miR-200ab/429 cluster, miR-10b, miR-193b, miR-141/200c, and miR-23b/27b). Regarding CD30+ cALCL, 39 differentially expressed microRNAs were identified. Particularly, overexpression of miR-155, miR-21, or miR-142-3p/5p and downregulation of the miR-141/200c clusters were observed. DNA methylation in microRNA gene promoters, as expression regulatory mechanism for deregulated microRNAs, was analyzed using Infinium 450K array and approximately one-third of the differentially expressed microRNAs showed significant DNA methylation differences. Two different microRNA methylation signatures for MFt and CD30+ cALCL were found. Correlation analysis showed an inverse relationship for microRNA promoter methylation and microRNA expression. These results reveal a subgroup-specific epigenetically regulated microRNA signatures for MFt and CD30+ cALCL patients.
Note: Versió postprint del document publicat a:
It is part of: Journal of Investigative Dermatology, 2015, vol. 135, num. 4, p. 1128-1137
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ISSN: 0022-202X
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Medicina)
Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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