Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/111087
Title: The chromatin remodeller CHD8 is required for E2F-dependent transcription activation of S-phase genes
Author: Subtil-Rodríguez, Alicia
Vázquez-Chávez, Elena
Ceballos-Chávez, María
Rodríguez-Paredes, Manuel
Martín-Subero, José Ignacio
Esteller, Manel
Reyes, José C.
Keywords: Cromatina
Transcripció genètica
Fixació de proteïnes
Cicle cel·lular
Proliferació cel·lular
Histones
Chromatin
Genetic transcription
Protein binding
Cell cycle
Cell proliferation
Histones
Issue Date: Feb-2014
Publisher: Oxford University Press
Abstract: The precise regulation of S-phase-specific genes is critical for cell proliferation. How the repressive chromatin configuration mediated by the retinoblastoma protein and repressor E2F factors changes at the G1/S transition to allow transcription activation is unclear. Here we show ChIP-on-chip studies that reveal that the chromatin remodeller CHD8 binds ∼2000 transcriptionally active promoters. The spectrum of CHD8 target genes was enriched in E2F-dependent genes. We found that CHD8 binds E2F-dependent promoters at the G1/S transition but not in quiescent cells. Consistently, CHD8 was required for G1/S-specific expression of these genes and for cell cycle re-entry on serum stimulation of quiescent cells. We also show that CHD8 interacts with E2F1 and, importantly, loading of E2F1 and E2F3, but not E2F4, onto S-specific promoters, requires CHD8. However, CHD8 recruiting is independent of these factors. Recruiting of MLL histone methyltransferase complexes to S-specific promoters was also severely impaired in the absence of CHD8. Furthermore, depletion of CHD8 abolished E2F1 overexpression-dependent S-phase stimulation of serum-starved cells, highlighting the essential role of CHD8 in E2F-dependent transcription activation.
Note: Reproducció del document publicat a: https://doi.org/10.1093/nar/gkt1161
It is part of: Nucleic Acids Research, 2014, vol. 42, num. 4, p. 2185-2196
Related resource: https://doi.org/10.1093/nar/gkt1161
URI: http://hdl.handle.net/2445/111087
ISSN: 0305-1048
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Fonaments Clínics)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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