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http://hdl.handle.net/2445/111087
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DC Field | Value | Language |
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dc.contributor.author | Subtil-Rodríguez, Alicia | - |
dc.contributor.author | Vázquez-Chávez, Elena | - |
dc.contributor.author | Ceballos-Chávez, María | - |
dc.contributor.author | Rodríguez-Paredes, Manuel | - |
dc.contributor.author | Martín-Subero, José Ignacio | - |
dc.contributor.author | Esteller, Manel | - |
dc.contributor.author | Reyes, José C. | - |
dc.date.accessioned | 2017-05-16T11:38:16Z | - |
dc.date.available | 2017-05-16T11:38:16Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/2445/111087 | - |
dc.description.abstract | The precise regulation of S-phase-specific genes is critical for cell proliferation. How the repressive chromatin configuration mediated by the retinoblastoma protein and repressor E2F factors changes at the G1/S transition to allow transcription activation is unclear. Here we show ChIP-on-chip studies that reveal that the chromatin remodeller CHD8 binds ∼2000 transcriptionally active promoters. The spectrum of CHD8 target genes was enriched in E2F-dependent genes. We found that CHD8 binds E2F-dependent promoters at the G1/S transition but not in quiescent cells. Consistently, CHD8 was required for G1/S-specific expression of these genes and for cell cycle re-entry on serum stimulation of quiescent cells. We also show that CHD8 interacts with E2F1 and, importantly, loading of E2F1 and E2F3, but not E2F4, onto S-specific promoters, requires CHD8. However, CHD8 recruiting is independent of these factors. Recruiting of MLL histone methyltransferase complexes to S-specific promoters was also severely impaired in the absence of CHD8. Furthermore, depletion of CHD8 abolished E2F1 overexpression-dependent S-phase stimulation of serum-starved cells, highlighting the essential role of CHD8 in E2F-dependent transcription activation. | - |
dc.format.extent | 12 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Oxford University Press | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1093/nar/gkt1161 | - |
dc.relation.ispartof | Nucleic Acids Research, 2014, vol. 42, num. 4, p. 2185-2196 | - |
dc.relation.uri | https://doi.org/10.1093/nar/gkt1161 | - |
dc.rights | cc-by-nc (c) Subtil-Rodríguez, Alicia et al., 2014 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Cromatina | - |
dc.subject.classification | Transcripció genètica | - |
dc.subject.classification | Fixació de proteïnes | - |
dc.subject.classification | Cicle cel·lular | - |
dc.subject.classification | Proliferació cel·lular | - |
dc.subject.classification | Histones | - |
dc.subject.other | Chromatin | - |
dc.subject.other | Genetic transcription | - |
dc.subject.other | Protein binding | - |
dc.subject.other | Cell cycle | - |
dc.subject.other | Cell proliferation | - |
dc.subject.other | Histones | - |
dc.title | The chromatin remodeller CHD8 is required for E2F-dependent transcription activation of S-phase genes | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 642636 | - |
dc.date.updated | 2017-05-16T11:38:17Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 24265227 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Fonaments Clínics) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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File | Description | Size | Format | |
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642636.pdf | 4.49 MB | Adobe PDF | View/Open |
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