Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/111429
Title: | Online haemodiafiltration improves inflammatory state in dialysis patients: A longitudinal study |
Author: | Rama, Inés Llaudó Vallmajor, Inés Fontova, Pere Cerezo, Gema Soto, Carlos Javierre Garcés, Casimiro F. Hueso Val, Miguel Montero Pérez, Núria Martínez Castelao, Alberto Torras Ambròs, Joan Grinyó Boira, Josep M. Cruzado, Josep Ma. Lloberas Blanch, Núria |
Keywords: | Diàlisi Hemodiàlisi Cèl·lules dendrítiques Urèmia Malalties hematològiques Nefrologia Dialysis Hemodialysis Dendritic cells Uremia Hematologic diseases Nephrology |
Issue Date: | 26-Oct-2016 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Background: Patients undergoing conventiona l hemodialysis (C-HD) present a greater immuno-inflam- matory state probably related to uremia, sympathetic nervous system (SNS) activation and /or membrane bioincompat ibility, which could improve with a technique-switch ing to online hemodiafiltration (OL-HD). The antigen-indep endent pathway activation of this modified immunologic state turns dendritic cells (DC) into an accurate cell model to study these patients. The aim of this study is to further evaluate the immune-inflammat ory state of patients in C-HD assessed by DC maturation. Methods: 31 patients were submitted to C-HD and after 4 months switched to the OL-HD technique. Monocytes-derive d DCs from HD patients were cultured in the presence of IL-4/GM-CSF. DC-maturation was evaluated by assessing the maturation phenotype by flow cytometry (FACs). DCs-functiona l capacity to elicit T-cell alloresponse was studied by mixed leuco- cyte reaction. Cytokine release was assessed by FACs and SNS was evaluated measuring renalase levels by ELISA. Results: An up-regulation of maturation markers was observed in C-HD DCs which induced two fold more T cells proliferation than OL-HD DCs. Also, C-HD-mDCs presented with over-produc- tion of pro-inflammatory cytokines (IL-6, IL-1 β , IL-8, IL-10 and TNF- α ) compared with OL- HD-mDC (P < 0·05). Results were correlated with clinical data. When SNS was evaluated, hypotension events and blood pressure were significantly lower and renalase levels were significantly higher after conversion to OL-HD. Diabetes mellitus type 2 patients also found beneficial reduction of mDC when converted to OL-HD compared to non-diabetics. Conclusions: OL-HD could interfere with immuno-inflammatory state in HD patients with an improvement of renalase levels as potential key mediators in the mechanistic pathway of down-regulation of DC maturation |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0164969 |
It is part of: | PLoS One, 2016, vol. 11, num. 10, p. e0164969 |
URI: | http://hdl.handle.net/2445/111429 |
Related resource: | https://doi.org/10.1371/journal.pone.0164969 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
664764.pdf | 2.32 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License