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http://hdl.handle.net/2445/112377
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DC Field | Value | Language |
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dc.contributor.author | Campillo, Noelia | - |
dc.contributor.author | Jorba, Ignasi | - |
dc.contributor.author | Schaedel, Laura | - |
dc.contributor.author | Casals, Blai | - |
dc.contributor.author | Gozal, David | - |
dc.contributor.author | Farré Ventura, Ramon | - |
dc.contributor.author | Almendros López, Isaac | - |
dc.contributor.author | Navajas Navarro, Daniel | - |
dc.date.accessioned | 2017-06-14T14:48:33Z | - |
dc.date.available | 2017-06-14T14:48:33Z | - |
dc.date.issued | 2016-07-29 | - |
dc.identifier.issn | 1664-042X | - |
dc.identifier.uri | http://hdl.handle.net/2445/112377 | - |
dc.description.abstract | Intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), plays a critical role in the pathogenesis of OSA-associated morbidities, especially in the cardiovascular and respiratory systems. Oxidative stress and inflammation induced by IH are suggested as main contributors of end-organ dysfunction in OSA patients and animal models. Since the molecular mechanisms underlying these in vivo pathological responses remain poorly understood, implementation of experimental in vitro cell-based systems capable of inducing high-frequency IH would be highly desirable. Here, we describe the design, fabrication, and validation of a versatile chip for subjecting cultured cells to fast changes in gas partial pressure and to cyclic stretch. The chip is fabricated with polydimethylsiloxane (PDMS) and consists of a cylindrical well-covered by a thin membrane. Cells cultured on top of the membrane can be subjected to fast changes in oxygen concentration (equilibrium time similar to 6 s). Moreover, cells can be subjected to cyclic stretch at cardiac or respiratory frequencies independently or simultaneously. Rat bone marrow-derived mesenchymal stem cells (MSCs) exposed to IH mimicking OSA and cyclic stretch at cardiac frequencies revealed that hypoxia-inducible factor 1 alpha (HIF-1 alpha) expression was increased in response to both stimuli. Thus, the chip provides a versatile tool for the study of cellular responses to cyclical hypoxia and stretch. | - |
dc.format.extent | 12 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3339/fphys.2016.00319 | - |
dc.relation.ispartof | Frontiers in Physiology, 2016, vol. 7, num. 319 | - |
dc.relation.uri | https://doi.org/10.3339/fphys.2016.00319 | - |
dc.rights | cc-by (c) Campillo, Noelia et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Síndromes d'apnea del son | - |
dc.subject.classification | Oxigen en l'organisme | - |
dc.subject.classification | Respiració | - |
dc.subject.other | Sleep apnea syndromes | - |
dc.subject.other | Oxygen in the body | - |
dc.subject.other | Respiration | - |
dc.title | A Novel chip for cyclic stretch and intermittent hypoxia cell exposures mimicking obstructive sleep apnea | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 668392 | - |
dc.date.updated | 2017-06-14T14:48:33Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27524971 | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
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668392.pdf | 3.89 MB | Adobe PDF | View/Open |
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