Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/112556
Title: i-motif structures in long cytosine-rich sequences found upstream of the promoter region of the SMARCA4 gen
Author: Benabou, Sanae
Aviñó Andrés, Anna
Lyonnais, Sébastien
González, Carlos
Eritja i Casadellà, Ramon
Juan Capdevila, Anna de
Gargallo Gómez, Raimundo
Keywords: Lligands
Oligonucleòtids
ADN
Ressonància magnètica nuclear
Cromatografia
Anàlisi multivariable
Ligands
Oligonucleotides
DNA
Nuclear magnetic resonance
Chromatography
Multivariate analysis
Issue Date: 12-Jun-2017
Publisher: Elsevier Masson SAS
Abstract: Cytosine-rich oligonucleotides are capable of forming complex structures known as i-motif with increasingly studied biological properties. The study of sequences prone to form i-motifs located near the promoter region of genes may be difficult because these sequences not only contain repeats of cytosine tracts of disparate length but also these may be separated by loops of varied nature and length. In this work, the formation of an intramolecular i-motif structures by a long sequence located upstream of the promoter region of the SMARCA4 gene has been demonstrated. Nuclear Magnetic Resonance, Circular Dichroism, Gel Electrophoresis, Size-Exclusion Chromatography, and multivariate analysis have been used. Not only the wild sequence (5'-TC3T2GCTATC3TGTC2TGC2TCGC3T2G2TCATGA2C4-3') has been studied but also several other truncated and mutated sequences. Despite the apparent complex sequence, the results showed that the wild sequence may form a relatively stable and homogeneous unimolecular i-motif structure, both in terms of pH or temperature. The model ligand TMPyP4 destabilizes the structure, whereas the presence of 20% (w/v) PEG200 stabilized it slightly. This finding opens the door to the study of the interaction of these kind of i-motif structures with stabilizing ligands or proteins.
Note: Versió postprint del document publicat a: https://doi.org/10.1016/j.biochi.2017.06.005
It is part of: Biochimie, 2017, vol. 140, p. 20-33
Related resource: https://doi.org/10.1016/j.biochi.2017.06.005
URI: http://hdl.handle.net/2445/112556
ISSN: 0300-9084
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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