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http://hdl.handle.net/2445/112611
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DC Field | Value | Language |
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dc.contributor.author | Tassinari, Eleonora | - |
dc.contributor.author | Aznar Vicente, Sonia | - |
dc.contributor.author | Urcola, Imanol | - |
dc.contributor.author | Prieto Durán, Alejandro | - |
dc.contributor.author | Hüttener Queiroz, Mário | - |
dc.contributor.author | Juárez Giménez, Antonio | - |
dc.date.accessioned | 2017-06-20T11:11:05Z | - |
dc.date.available | 2017-06-20T11:11:05Z | - |
dc.date.issued | 2016-05-03 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/2445/112611 | - |
dc.description.abstract | IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2016.00629 | - |
dc.relation.ispartof | Frontiers in Microbiology, 2016, vol. 7, num. 6, p. 629 | - |
dc.relation.uri | https://doi.org/10.3389/fmicb.2016.00629 | - |
dc.rights | cc-by (c) Tassinari, Eleonora et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Seqüència d'aminoàcids | - |
dc.subject.classification | Plasmidis | - |
dc.subject.other | Amino acid sequence | - |
dc.subject.other | Plasmids | - |
dc.title | The incC sequence is required for R27 plasmid stability | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 671005 | - |
dc.date.updated | 2017-06-20T11:11:05Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27199955 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
Files in This Item:
File | Description | Size | Format | |
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671005.pdf | 650.57 kB | Adobe PDF | View/Open |
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