Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/113346
Title: DYRK1A-mediated phosphorylation of GluN2A at Ser1048 regulates the surface expression and channel activity of GluN1/GluN2A receptors
Author: Grau, Cristina
Arató, Krisztina
Fernández-Fernández, José Manuel
Valderrama, Aitana
Sindreu Balet, Carlos
Fillat i Fonts, Cristina
Ferrer, Isidro (Ferrer Abizanda)
Luna, Susana de la
Altafaj, Xavier
Keywords: Aminoàcids
Sinapsi
Transport biològic
Neurobiologia del desenvolupament
Síndrome de Down
Rates (Animals de laboratori)
Amino acids
Synapses
Biological transport
Developmental neurobiology
Down syndrome
Rats as laboratory animals
Issue Date: 17-Oct-2014
Publisher: Frontiers Media
Abstract: N-methyl-D-aspartate glutamate receptors (NMDARs) play a pivotal role in neural development and synaptic plasticity, as well as in neurological disease. Since NMDARs exert their function at the cell surface, their density in the plasma membrane is finely tuned by a plethora of molecules that regulate their production, trafficking, docking and internalization in response to external stimuli. In addition to transcriptional regulation, the density of NMDARs is also influenced by post-translational mechanisms like phosphorylation, a modification that also affects their biophysical properties. We previously described the increased surface expression of GluN1/GluN2A receptors in transgenic mice overexpressing the Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A), suggesting that DYRK1A regulates NMDARs. Here we have further investigated whether the density and activity of NMDARs were modulated by DYRK1A phosphorylation. Accordingly, we show that endogenous DYRK1A is recruited to GluN2A-containing NMDARs in the adult mouse brain, and we identify a DYRK1A phosphorylation site at Ser(1048) of GluN2A, within its intracellular C-terminal domain. Mechanistically, the DYRK1A-dependent phosphorylation of GluN2A at Ser(1048) hinders the internalization of GluN1/GluN2A, causing an increase of surface GluN1/GluN2A in heterologous systems, as well as in primary cortical neurons. Furthermore, GluN2A phosphorylation at Ser(1048) increases the current density and potentiates the gating of GluN1/GluN2A receptors. We conclude that DYRK1A is a direct regulator of NMDA receptors and we propose a novel mechanism for the control of NMDAR activity in neurons.
Note: Reproducció del document publicat a: https://doi.org/10.3389/fncel.2014.00331
It is part of: Frontiers in Cellular Neuroscience, 2014, vol. 8, p. 1
Related resource: https://doi.org/10.3389/fncel.2014.00331
URI: http://hdl.handle.net/2445/113346
ISSN: 1662-5102
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Fonaments Clínics)

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