Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/113442
Title: Minimal genetic change in Vibrio cholerae in Mozambique over time: Multilocus variable number tandem repeat analysis and whole genome sequencing
Author: Garrine, Marcelino
Mandomando, Inácio
Vubil, Delfino
Nhampossa, Tacilta
Acácio, Sozinho
Li, Shan
Paulson, Joseph N.
Almeida, Mathieu
Domman, Daryl
Thomson, Nicholas R.
Alonso, Pedro
Stine, Oscar C.
Keywords: Còlera
Moçambic
Cholera
Mozambique
Issue Date: 16-Jun-2017
Publisher: Public Library of Science
Abstract: Although cholera is a major public health concern in Mozambique, its transmission patterns remain unknown. We surveyed the genetic relatedness of 75 Vibrio cholerae isolates from patients at Manhica District Hospital between 2002-2012 and 3 isolates from river using multilocus variable-number tandem-repeat analysis (MLVA) and whole genome sequencing (WGS). MLVA revealed 22 genotypes in two clonal complexes and four unrelated genotypes. WGS revealed i) the presence of recombination, ii) 67 isolates descended monophyletically from a single source connected to Wave 3 of the Seventh Pandemic, and iii) four clinical isolates lacking the cholera toxin gene. This Wave 3 strain persisted for at least eight years in either an environmental reservoir or circulating within the human population. Our data raises important questions related to where these isolates persist and how identical isolates can be collected years apart despite our understanding of high change rate of MLVA loci and the V. cholerae molecular clock.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pntd.0005671
It is part of: PLoS Neglected Tropical Diseases, 2017, vol. 11, num. 6, p. e0005671
Related resource: http://dx.doi.org/10.1371/journal.pntd.0005671
URI: http://hdl.handle.net/2445/113442
ISSN: 1935-2727
Appears in Collections:Articles publicats en revistes (ISGlobal)

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