Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/113565
Title: DNA methylation alterations in grade II- and anaplastic pleomorphic xanthoastrocytoma
Author: Martínez, Ramón
Carmona, F. Javier
Vizoso, Miguel
Rohde, Veit
Kirsch, Matthias
Schackert, Gabriele
Ropero, Santiago
Paulus, Werner
Barrantes, Alonso
Gomez, Antonio
Esteller, Manel
Keywords: ADN
Metilació
Genètica
Càncer
Tumors
DNA
Methylation
Genetics
Cancer
Tumors
Issue Date: 20-Mar-2014
Publisher: BioMed Central
Abstract: Background: Pleomorphic xanthoastrocytoma (PXA) is a rare WHO grade II tumor accounting for less than 1% of all astrocytomas. Malignant transformation into PXA with anaplastic features, is unusual and correlates with poorer outcome of the patients. Methods: Using a DNA methylation custom array, we have quantified the DNA methylation level on the promoter sequence of 807 cancer-related genes of WHO grade II (n = 11) and III PXA (n = 2) and compared to normal brain tissue (n = 10) and glioblastoma (n = 87) samples. DNA methylation levels were further confirmed on independent samples by pyrosequencing of the promoter sequences. Results: Increasing DNA promoter hypermethylation events were observed in anaplastic PXA as compared with grade II samples. We further validated differential hypermethylation of CD81, HCK, HOXA5, ASCL2 and TES on anaplastic PXA and grade II tumors. Moreover, these epigenetic alterations overlap those described in glioblastoma patients, suggesting common mechanisms of tumorigenesis. Conclusions: Even taking into consideration the small size of our patient populations, our data strongly suggest that epigenome-wide profiling of PXA is a valuable tool to identify methylated genes, which may play a role in the malignant progression of PXA. These methylation alterations may provide useful biomarkers for decision-making in those patients with low-grade PXA displaying a high risk of malignant transformation.
Note: Reproducció del document publicat a: https://doi.org/10.1186/1471-2407-14-213
It is part of: BMC Cancer, 2014, vol. 14, p. 213
Related resource: https://doi.org/10.1186/1471-2407-14-213
URI: http://hdl.handle.net/2445/113565
ISSN: 1471-2407
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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